Experience in Northern Nigeria with countercurrent immunoelectrophoresis, double diffusion and indirect haemagglutination tests for diagnosis of hydatid cyst in camels

Dada, B. J. O.; Adegboye, D. S.; Mohammed, AZ

doi:10.1017/s0022149x00026857

Cited by 7 publications

(4 citation statements)

References 9 publications

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“…An IHA using crude hydatid cyst fluid antigen was reported to have 72% sensitivity for camel CE in Nigeria (Dada et al, 1981), and Ibrahem et al (2002) obtained 43% sensitivity and 69% specificity with IHA for CE in Sudanese camels. The sensitivity and specificity of IHA were 87.3 and 80.9%, respectively.…”

Section: Discussionmentioning

confidence: 96%

“…This shows a better diagnostic performance than similar tests performed elsewhere using the same antigen source but on different species of animals. An IHA using crude hydatid cyst fluid antigen was reported to have 72% sensitivity for camel CE in Nigeria (Dada et al , 1981), and Ibrahem et al (2002) obtained 43% sensitivity and 69% specificity with IHA for CE in Sudanese camels. A much lower sensitivity (27%) with IHA for sera from camel CE was reported from Egypt (Ibrahem et al , 1996).…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of crude hydatid cyst fluid antigens for the serological diagnosis of hydatidosis in cattle

2010

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Echinococcosis is a zoonotic infection caused by adult or larval (metacestode) stages of cestodes belonging to the genus Echinococcus. The purpose of this study was to evaluate the antigenic ability of hydatid cyst fluid antigen for the diagnosis of hydatidosis in cattle using enzyme-linked immunosorbent assay (ELISA) and indirect haemagglutination test (IHA). The source of the antigens for the serological tests was fertile crude cyst fluids collected from naturally infected sheep at the Addis Ababa abattoir. A total of 502 sera were collected from 329 uninfected cattle and 173 hydatid-infected cattle which were confirmed by post-mortem examination. Most cysts were sterile and multiple organ infection predominated. Of 173 infected cattle, 166 (96.0%; confidence interval (CI) 91.8-98.4) were positive using ELISA while 7 (4.0%) were negative. Of 329 sera from uninfected cattle, 274 (83.3%; CI 78.8-87.2) were found to be negative and the remaining 55 (16.7%) were positive by ELISA. Of 173 infected cattle, 151 (87.3%; CI 81.4-91.9) were positive and 22 (12.7%) were negative by IHA. Of 329 negative sera tested using IHA, 266 (80.9%; CI 76.2-85.0) were negative and the remaining 63 (19.1%) were positive. The false positive and negative values of ELISA were 4.0 and 16.7%, respectively, and the corresponding values of IHA were 12.7 and 19.1%. The sensitivity and diagnostic efficiency of IHA were 87.2 and 83.6%, respectively. Crude hydatid cyst fluid antigen seems to have reasonable antigenic properties and hence could be employed for epidemiological surveillance of cattle hydatidosis.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Evaluation of crude hydatid cyst fluid antigens for the serological diagnosis of hydatidosis in cattle

2010

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“…Hydatid cysts are usually identified during the slaughter of domestic animals at slaughterhouses. However, serological methods have been used to diagnose hydatidosis in domestic animals (Dada and Belino, 1978;Dada et al, 1981;Haroun et al, 2008;Sazmand et al, 2013;Igwenagu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

The global status and genetic characterization of hydatidosis in camels (Camelus dromedarius): a systematic literature review with meta-analysis based on published papers

et al. 2020

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Hydatidosis is a potential zoonotic helminthic disease affecting a broad spectrum of mammals, including humans, worldwide. The current review was conducted to investigate the genotypic status and prevalence of hydatid disease in camels across the world. For the purpose of the study, the articles addressing the worldwide prevalence of hydatidosis in camels were searched in several English language databases. The search process resulted in the inclusion of 122 papers. Based on the data presented in the reviewed articles, the pooled prevalence of hydatid disease in camels across the world was measured at 23.75% (95% CI 20.15–27.55). Moreover, the subgroup analysis demonstrated significant differences in the overall prevalence of hydatidosis among camels based on year, geographic area, climate parameters, camel population, gender, infected organ, fertility rate of the cyst and laboratory diagnostic technique. Furthermore, the Echinococcus granulosus genotypes identified in camels with hydatidosis included G1, G2, G3, G1–G3, G5, G6, G7, G6–G7 and G6–G10, with G6 being the most common genotype throughout the world. The data obtained from the current study are central to the better conceptualization of the biological and epidemiological characteristics of E. granulosus s.l. genotypes around the world, which can be helpful in the planning and adoption of more comprehensive control strategies.

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“…Despite the potential value of serological diagnostic tests for hydatid control programs, Hydatidosis has been studied extensively in humans than animals (Rickard and Lightowlers, 1986). Compliment fixation tests, agglutination tests, double gel diffusion immunoelectrophoresis and the enzyme-linked immunosorbent assay (ELISA) have all been used in the serodiagnosis of hydatidosis in sheep and other ruminants (Craig et al 1981;Dada et al 1981;Lightowlers et al 1984;Kittelberger et al 2002), offering better results with the use of the B/5 rich fraction or partly purified antigen from hydatid fluid (Rogan et al 1991;Sbihi et al1996).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Enzyme -Linked Immunosorbent Assay (ELISA) and Western Blotting for the immunodiagnosis of hydatid diseases in Sheep and Goats

2009

IJVM

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CitationS Luka, I Ajogi, I Nock, C Kudi, J Umoh. Evaluation of Enzyme -Linked Immunosorbent Assay (ELISA) and Western Blotting for the immunodiagnosis of hydatid diseases in Sheep and Goats. AbstractThis study was undertaken to investigate antigenic characteristics of hydatid cyst fluid in sheep and goats by Sodium Dodecyl Sulphate-Poly Acrylamide Gel Electrophoresis (SDS-PAGE), to evaluate the sensitivity and specificity of Enzyme Linked Immunosorbent Assay (ELISA) and Western blotting for the diagnosis and determination of seroprevalence of hydatidosis in sheep and goats slaughtered in Kano Abattoir, Northern Nigeria. The SDS-PAGE analysis of sheep hydatid cyst fluids indicated that 8 specific-protein bands were detected at molecular weights of 16, 24, 36, 52, 66, 96, 118 and 150kDa while the goat purified HCF showed 4 specific protein bands of 45, 52, 100 and 118kDa. Western blot analysis showed the dorminant components of 12, 45, 50-75 and 100-160kDa in sheep and goats HCF. The most consistent demonstrable protein in Echinococcus granulosus occurred as a complex in the 52-62kDa region. Bands of 66,118 and 150kDa persisted among sera of infected animals. The sensitivity and specificity of ELISA were determined as 66% and 86% in sheep and 54% and 73% in goats. Where as corresponding rates for western blotting were determined as 71% and 65% in sheep and 69% and 72% in goats. Out of a total of 268 sera obtained from 130 goats and 136 sheep, 31(23.8%) goats and 50(36.2%) sheep were seropositive, higher prevalences were recorded among older sheep than goats.The study concludes that serology is a useful diagnostic tool for hydatidosis and underscores the need for such standard laboratory facilities in the educational and health sectors for establishing the status of hydatid disease in Nigeria.

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Experience in Northern Nigeria with countercurrent immunoelectrophoresis, double diffusion and indirect haemagglutination tests for diagnosis of hydatid cyst in camels

Cited by 7 publications

References 9 publications

Evaluation of crude hydatid cyst fluid antigens for the serological diagnosis of hydatidosis in cattle

Evaluation of crude hydatid cyst fluid antigens for the serological diagnosis of hydatidosis in cattle

The global status and genetic characterization of hydatidosis in camels (Camelus dromedarius): a systematic literature review with meta-analysis based on published papers

Evaluation of Enzyme -Linked Immunosorbent Assay (ELISA) and Western Blotting for the immunodiagnosis of hydatid diseases in Sheep and Goats

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