Experiment-Based Validation of Corneal Lenticule Banking in a Health Authority-Licensed Facility

Riau, Andri K.; Boey, Kenny P Y; Yusoff, Nur Zahirah Binte M; Goh, Tze-Wei; Yam, Gary Hin‐Fai; Tang, Kin Fai; Phua, Catherine S H; Chen, Huijun; Chiew, Yoke F; Liu, Yu-Chi

doi:10.1089/ten.tea.2021.0042

Cited by 11 publications

(3 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chen medium also received FDA approval for corneal storage, and storage of corneal tissue in it can result in minimal injury for up to 14 days. 26 It is a feasible approach for the storage of stromal lenticules after refractive surgery to build long-term lenticule banking based on these methods, 27 and some countries have already established relatively mature lenticule banking. In addition, it has been reported that short-term storage of SMILE lenticules in phosphate buffer saline (PBS) has no significant effect, at least for the first 48 hours at 4 °C or room temperature before transportation to technical facilities for long-term storage, 28 making emergency preservation of lenticules possible under nonprofessional conditions.…”

Section: Corneal Potassium Tes (Cptes)mentioning

confidence: 99%

Update of Research Progress on Small Incision Lenticule Extraction (SMILE) Lenticule Reuse

Deng¹,

Li²,

Tang³

2023

OPTH

View full text Add to dashboard Cite

The SMILE lenticule is a complete corneal stroma that is removed from SMILE surgery. Since the increasing number of SMILE surgeries, a large number of SMILE lenticules have been produced, so the reuse and preservation of the stromal lens has become a research hotspot. Due to the rapid development of the preservation and clinical reuse of SMILE lenticules, there have been many related studies in recent years, so we updated it on this basis. We searched PubMed, Web of Science, Embase, Elsevier Science, CNKI, WANFANG Data and other databases for all articles published on the preservation and clinical reuse of SMILE lenticules, screened useful articles, selected relevant articles published in the last five years as the main body for summary, and then reached a conclusion. The existing preservation methods of SMILE lenticule include Moist chamber storage at low temperature, cryopreservation technique dehydrating agent and corneal storage medium, which have their own advantages and disadvantages. Presently, smile lenticules can be used for the treatment of corneal ulcers and perforations, corneal tissue defects, hyperopia, presbyopia and keratectasia, which have been proven to be relatively effective and safe. More research on smile lenticule reuse needs to be carried out to confirm its long-term efficacy.

show abstract

Section: Corneal Potassium Tes (Cptes)mentioning

confidence: 99%

Update of Research Progress on Small Incision Lenticule Extraction (SMILE) Lenticule Reuse

Deng¹,

Li²,

Tang³

2023

OPTH

View full text Add to dashboard Cite

show abstract

“…To this end, our attention turned to the by-product of a refractive surgical procedure known as SMILE and the ultra-thin lenticules extracted from the corneal stroma of individuals that undergo this elective procedure. Given that at least 5 million SMILE surgeries have been conducted worldwide (https://eyewire.news/news/zeiss-marks-milestonewith-more-than-5-million-eyes-treated-with-smile?c4src = article:infinite-scroll) and the utility of lenticules in the treatment of a variety of ocular diseases, [19][20][21][22]38] there is growing interest in developing preservation protocols [39,40] and banking systems [41,42] for this valuable tissue resource.…”

Section: Clecs Cultured On Smile-derived Lenticulesmentioning

confidence: 99%

A Bioengineering‐Regenerative Medicine Approach for Ocular Surface Reconstruction Using a Functionalized Native Cornea‐Derived Bio‐Scaffold

Park,

Richardson,

Delic

et al. 2023

Adv Funct Materials

View full text Add to dashboard Cite

Limbal stem cell deficiency (LSCD) is characterized by the loss of limbal epithelial stem cells (LESCs) which compromises corneal transparency, leading to blindness. It cannot be treated with pharmacological or corneal transplantation interventions, instead a specialized stem cell (SC) therapy is needed to restore eye health and sight. Herein, a native cornea‐derived biomaterial, a by‐product of a laser refractive surgical procedure called small incision lenticule extraction is identified as a new cell delivery matrix. Culture conditions are optimized to facilitate LESC attachment, expansion and stratification, and their identity is immunophenotyped. Using electron microscopy, bio‐constructs display stratification, similar to the architectural and cellular organization of a native mammalian cornea with formation of a basement membrane and an orderly array of collagen fibrils. Neuronal growth and depleted CD45+/CD14+ leukocytes on lenticules are also shown, suggesting that in transplantation experiments, they will re‐innervate and not trigger a host‐mediated immune response. Finally, human lenticules are geometrically customized to successfully fit them over a LSCD murine cornea ex vivo, during which they maintain curvature. The authors are poised to conduced similar studies in live mice using these and other carriers currently used in the clinic to compare SC therapy outcomes.

show abstract

“…Lenticule storage and biobanking systems are still being developed in a few countries, such as Singapore (https://www.straitstimes.com/singapore/health/singaporelaunches-first-bank-in-asia-for-eye-surgery-patients-to-freeze-piece-of, accessed on 3 July 2022) [87,95]. A structured regulatory and organizational framework for lenticule processing and banking is important for the successful operation, standardization, and quality assurance of lenticule products and for safe and effective treatments for patients.…”

mentioning

confidence: 99%

Human SMILE-Derived Stromal Lenticule Scaffold for Regenerative Therapy: Review and Perspectives

Santra

Liu

Jhanji

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

A transparent cornea is paramount for vision. Corneal opacity is one of the leading causes of blindness. Although conventional corneal transplantation has been successful in recovering patients’ vision, the outcomes are challenged by a global lack of donor tissue availability. Bioengineered corneal tissues are gaining momentum as a new source for corneal wound healing and scar management. Extracellular matrix (ECM)-scaffold-based engineering offers a new perspective on corneal regenerative medicine. Ultrathin stromal laminar tissues obtained from lenticule-based refractive correction procedures, such as SMall Incision Lenticule Extraction (SMILE), are an accessible and novel source of collagen-rich ECM scaffolds with high mechanical strength, biocompatibility, and transparency. After customization (including decellularization), these lenticules can serve as an acellular scaffold niche to repopulate cells, including stromal keratocytes and stem cells, with functional phenotypes. The intrastromal transplantation of these cell/tissue composites can regenerate native-like corneal stromal tissue and restore corneal transparency. This review highlights the current status of ECM-scaffold-based engineering with cells, along with the development of drug and growth factor delivery systems, and elucidates the potential uses of stromal lenticule scaffolds in regenerative therapeutics.

show abstract

Experiment-Based Validation of Corneal Lenticule Banking in a Health Authority-Licensed Facility

Cited by 11 publications

References 48 publications

Update of Research Progress on Small Incision Lenticule Extraction (SMILE) Lenticule Reuse

Update of Research Progress on Small Incision Lenticule Extraction (SMILE) Lenticule Reuse

A Bioengineering‐Regenerative Medicine Approach for Ocular Surface Reconstruction Using a Functionalized Native Cornea‐Derived Bio‐Scaffold

Human SMILE-Derived Stromal Lenticule Scaffold for Regenerative Therapy: Review and Perspectives

Contact Info

Product

Resources

About