Experimental acrylonitrile butadiene styrene and polyamide evisceration implant: a rabbit clinical and histopathology study

Abstract

The purpose of this study was to evaluate acrylonitrile butadiene styrene (ABS) and polyamide implants in rabbits submitted to evisceration at the macroscopic and microstructure level and to assess clinical response and histopathological changes as well. For the experimental study implants of 12mm diameter were prepared by rapid prototyping, weighed and the outer and inner surfaces evaluated macroscopically and by electron microscopy. In addition, a compression test was performed and ultrastructural damage was then determined. After evisceration of the left eyeball, nine New Zealand rabbits received ABS implants and nine others received polyamide implants. The animals were assessed daily for 15 days after surgery and every seven days until the end of the study (90 days). Histopathological evaluation was performed at 15, 45 and 90 days after surgery. The ABS implants weighed approximately 0.44g, while the polyamide ones weighed 0.61g. Scanning electron microscopy demonstrated that the ABS implants had regular-sized, equidistant micropores, while the polyamide ones showed micropores of various sizes. The force required to fracture the ABS implant was 14.39 ±0.60 Mpa, while for the polyamide one, it was 16.80 ±1.05 Mpa. Fifteen days after surgery, we observed centripetal tissue infiltration and scarce inflammatory infiltrate. Implants may be used in the filling of anophthalmic cavities, because they are inert, biocompatible and allow tissue integration.Keywords: rabbits, ocular prosthesis, polymers RESUMO Avaliou-se a macroscopia, a microestrutura, a resposta clínica e histopatológica de implantes de acrilonitrila butadieno estireno (ABS) e poliamida em coelhos submetidos à evisceração. Para o estudo experimental, os implantes foram elaborados por meio de prototipagem rápida, com 12mm de diâmetro, pesados e tiveram suas superfícies avaliadas macroscopicamente e por microscopia eletrônica de varredura. Adicionalmente, foi realizado ensaio de compressão para determinar a força necessária para fraturar os implantes. Após a evisceração do olho esquerdo, nove coelhos da raça Nova Zelândia receberam implantes de poliamida e nove outros receberam implantes de ABS. Os animais foram avaliados diariamente nos primeiros 15 dias após a cirurgia e a cada sete dias até o fim do período experimental (90 dias

A novel method for high precision aortic constriction that allows for generation of specific cardiac phenotypes in mice

“…Moreover, the inert nitrile rubber material of the o-rings did not cause any adverse effects upon insertion, in line with other studies where acrylonitrile-butadiene polymers have been inserted in rats and rabbits. 42,43 Based on these traits, we speculate that ORAB could also serve as an improved debanding model to study reverse remodelling. Suture-based debanding models of reverse remodelling in mice do exist, 44,45 however the method is rarely used as the procedure must be performed frequently to maintain acceptable success rates.…”

Effect of Infill Patterns with Machine Learning Techniques on the Tensile Properties of Polylactic Acid-Based Ceramic Materials with Fused Filament Fabrication

“…The thermoplastic polymer styrene was also used. Gomes et al 5 tested the biocompatibility and bioinertness of the ABS polyamide on rabbits. After studying the ABS-M30i biocompatible material, researchers found that it is bioinert.…”