Experimental and Modeling Based Investigations of Process Parameters on a Novel, 3D Printed and Self-Insulated 24-Well, High-Throughput 3D Microelectrode Array Device for Biological Applications

Castro, Jorge Manrique; Rajaraman, Swaminathan

doi:10.1109/jmems.2022.3160663

Cited by 8 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The detailed microfabrication approach used for these HT-3D MEAs is described in our recently published IEEE JMEMS article [11]. Only a brief description of the methods used in this work are detailed below.…”

Section: Methodsmentioning

confidence: 99%

Constant Phase Element (Cpe) Modeling and Analysis of Multi-Material, Micro-Bullet Shaped, High-Throughput 3d Microelectrodes for in-Vitro Electrophysiological Applications

Manrique Castro,

Rajaraman

2022

2022 Solid-State, Actuators, and Microsystems Workshop Technical Digest

View full text Add to dashboard Cite

This paper reports for the first time, modeling, and analysis of Electrochemical Impedance Spectroscopy (EIS) from micro-bullet shaped 3D microelectrodes fabricated in high-throughput array (HT) formats with three different materials [Gold, Biocompatible-Silver Paste and Microelectronic-Silver Paste] in electrode-electrolyte interface. The modeling was based on the Randles' Electrochemical Equivalent Circuit (EEC) with a Constant Phase Element (CPE) driving element and impedance data collected from HT-3D Microelectrode Arrays (MEAs) was fitted. CPE was determined as an excellent adaptative element for electrical representation in MEA models due to low error per element during fitting process into EEC, and exceptional matching observed between modeling and experiments opening new avenues into the understanding of electrochemical interaction of 3D microelectrodes.

show abstract

Section: Methodsmentioning

confidence: 99%

Constant Phase Element (Cpe) Modeling and Analysis of Multi-Material, Micro-Bullet Shaped, High-Throughput 3d Microelectrodes for in-Vitro Electrophysiological Applications

Manrique Castro,

Rajaraman

2022

2022 Solid-State, Actuators, and Microsystems Workshop Technical Digest

View full text Add to dashboard Cite

show abstract

“…In recent years, there has been a rapid development of the 3D printing technique for the formation of microelectrode arrays (MEA) intended for recording the electrical activity of neurons and synapses in biological neural networks. [303] For the production of conducting electrode microstructures, inkjet, and EHD printing, [261,[304][305][306][307] stereolithography and multiphoton lithography [308][309][310][311] were used. As in cardiac OOC, brain-on-a-chip systems employ tissue cultivation directly on the electrodes.…”

Section: Neural Interfacesmentioning

confidence: 99%

3D Printed Biohybrid Microsystems

Prinz

Fritzler

2022

Adv Materials Technologies

View full text Add to dashboard Cite

are not able to fully meet the challenges related to the creation of such systems. A number of methods are known that make it possible to form 3D structures, for example, Rolling-Up technology and imprint lithography. [1][2][3][4] However, a rather short list of materials that can be used in such technologies and a too narrow range of 3D shapes that can be fabricated using these methods seriously limit the application fields of the techniques. It seems that additive manufacturing (AM) technology, or 3D printing, presents a much more universal and promising technological approach in this field. AM technology is the process of forming structures and devices by layer-by-layer (or pointby-point) application of materials in accordance with a 3D digital computer aided design (CAD) model. [5] The results of recent years convincingly show that the rapidly progressing AM technology, which uses the widest range of materials, including biological ones, has become the technological basis and the driving force of new breakthrough fields, including biology and medicine. [6,7] The purpose of our review is to analyze the role and achievements of 3D micro-and nanoprinting in the development of one of the most advanced fields, biohybrid systems (BHS), formed by the integration of at least one biological component with at least one artificial element. In the creation of such systems, the desire was manifested to combine the unique capabilities of living biosystems, having been perfected during the millions of years of evolution, with the functionality of artificially made structures. This integration leads to a synergistic effect and significantly expands the capabilities of the devices being created, which are in demand in various fields, primarily in biology and medicine. This review by no means aims to provide an exhaustive picture of the entire field of biohybrid materials and structures; instead, it is devoted to the consideration of smart biohybrid microsystems. These are complex multifunctional devices that allow recording the biophysical and biochemical parameters of biological objects and exerting active control on those parameters for treatment or research. [8][9][10][11][12][13] The functionality of such systems is provided by micro-and nanotools, for the production of which high-resolution additive methods are used. These are various optical, electronic, mechanical microand nanostructures that are used as activators, capsules for transporting cells or active substances, elements of microfluidic systems, sensors, microelectrode arrays, etc. The review addresses the formation and integration of such structures into smart biohybrid microsystems. The review outlines the most This review is devoted to the role of 3D printing in the development of a new high-tech field, smart biohybrid microsystems. The motivation behind the development of this field is the intention to integrate the capabilities of biological systems optimized in the course of evolution with the achievements of modern methods of forming micro-and nanostructu...

show abstract

“…[ 26 ] Moreover, stereolithography 3D printing has been used in combination with ink casting and electroplating of metal electrodes for the fabrication of 3D MEAs in well plates. [ 27 ] Nonetheless, these approaches have a limited printing resolution down to tens of micrometers, require conductive inks with a high Young's modulus that leads to a higher cross‐sectional footprint, and either lack or require an additional step to implement an insulating layer to passivate the printed electrodes.…”

Section: Introductionmentioning

confidence: 99%

Highly Customizable 3D Microelectrode Arrays for In Vitro and In Vivo Neuronal Tissue Recordings

Abu Shihada,

Jung,

Decke

et al. 2024

Advanced Science

View full text Add to dashboard Cite

Planar microelectrode arrays (MEAs) for – in vitro or in vivo – neuronal signal recordings lack the spatial resolution and sufficient signal‐to‐noise ratio (SNR) required for a detailed understanding of neural network function and synaptic plasticity. To overcome these limitations, a highly customizable three‐dimensional (3D) printing process is used in combination with thin film technology and a self‐aligned template‐assisted electrochemical deposition process to fabricate 3D‐printed‐based MEAs on stiff or flexible substrates. Devices with design flexibility and physical robustness are shown for recording neural activity in different in vitro and in vivo applications, achieving high‐aspect ratio 3D microelectrodes of up to 33:1. Here, MEAs successfully record neural activity in 3D neuronal cultures, retinal explants, and the cortex of living mice, thereby demonstrating the versatility of the 3D MEA while maintaining high‐quality neural recordings. Customizable 3D MEAs provide unique opportunities to study neural activity under regular or various pathological conditions, both in vitro and in vivo, and contribute to the development of drug screening and neuromodulation systems that can accurately monitor the activity of large neural networks over time.

show abstract

Experimental and Modeling Based Investigations of Process Parameters on a Novel, 3D Printed and Self-Insulated 24-Well, High-Throughput 3D Microelectrode Array Device for Biological Applications

Cited by 8 publications

References 22 publications

Constant Phase Element (Cpe) Modeling and Analysis of Multi-Material, Micro-Bullet Shaped, High-Throughput 3d Microelectrodes for in-Vitro Electrophysiological Applications

Constant Phase Element (Cpe) Modeling and Analysis of Multi-Material, Micro-Bullet Shaped, High-Throughput 3d Microelectrodes for in-Vitro Electrophysiological Applications

3D Printed Biohybrid Microsystems

Highly Customizable 3D Microelectrode Arrays for In Vitro and In Vivo Neuronal Tissue Recordings

Contact Info

Product

Resources

About