Experimental challenges of wild Manila clams with Perkinsus species isolated from naturally infected wild Manila clams

Waki, Tsukasa; Shimokawa, Jun; Watanabe, Shinji; Yoshinaga, Tomoyoshi; Ogawa, Kazuo

doi:10.1016/j.jip.2012.05.009

Cited by 44 publications

(22 citation statements)

References 17 publications

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“…Spats (3 -15 mm) of clams Ruditapes philippinarum also acquired infection with Perkinsus spp. (P. olseni dominant) after immersion challenge with zoospores and suffered a high mortality (SHIMOKAWA et al, 2010;WAKI et al, 2012). Concerning P. marinus, infection in C. virginica oysters smaller than 30 mm is rare (BURRESON, 1991).…”

Section: Discussionmentioning

confidence: 99%

Growth, mortality and susceptibility of oyster Crassostrea spp. to Perkinsus spp. infection during on growing in northeast Brazil

Scardua

Vianna

Duarte

et al. 2017

Rev. Bras. Parasitol. Vet.

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Crassostrea rhizophorae and C. gasar oysters are cultivated in the northeast region. Perkinsus parasites infect bivalves, and their effects on oysters from tropical regions are poorly understood. This study evaluated the impact of Perkinsus infection on the productive traits of native oysters. Oysters were sampled bimonthly during 7 months, from July 2010 to February 2011, to evaluate growth rate, mortality and shell color patterns (white and dark-gray) (n = 500), and to determine the prevalence and intensity of Perkinsus (n = 152). Perkinsus and Crassostrea species were determined using molecular tools. Results showed that most dark-gray (90%, n = 20) and white (67%, n = 18) oysters were C. gasar and C. rhizophorae, respectively. Oysters showed a high growth rate and moderate cumulative mortality (44%). C. gasar oysters grew better and showed lower mortality and lower incidence of Perkinsus compared to C. rhizophorae. The mean prevalence of Perkinsus was moderate (48%), but the infection intensity was light (2.2). Perkinsosis affected very small oysters (19.4 mm). In conclusion, native oysters, especially C. gasar, have a great potential for culture, mortality is not associated with perkinsosis, and the shell color of oysters can be used to improve selection for spats with better performance.

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Section: Discussionmentioning

confidence: 99%

Growth, mortality and susceptibility of oyster Crassostrea spp. to Perkinsus spp. infection during on growing in northeast Brazil

Scardua

Vianna

Duarte

et al. 2017

Rev. Bras. Parasitol. Vet.

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“…Diseases caused mainly by Perkinsus (Waki et al 2012) and Vibrio genera (Gestal et al 2008) are associated with significant economic losses. The development and health status of these organisms can be a Corresponding author: beatriznovoa@iim.csic.es especially affected by bacterial diseases (Allam et al 2000;Gómez-León et al 2005) which can alter the quality and price of the product.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of reference genes ofMytilus galloprovincialisandRuditapes philippinaruminfected with three bacteria strains for gene expression analysis

Moreira

Pereiro

Costa

et al. 2014

Aquat. Living Resour.

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-Quantitative real-time polymerase chain reaction (qPCR) is probably the most used method for gene expression quantification because of its high sensitivity and specificity. Nevertheless, this technology can undergo experimental errors and variations. Normalization of the results using a reference gene is therefore necessary to minimize these variations. As the study of immune genes in bivalve mollusks has increased in the last years, the establishment of adequate and stable reference genes for bivalves is strongly required. We analyzed the behavior of four putative reference genes: ribosomal RNA 18S, actin, elongation factor 1 − α and α-tubulin. The suitability of these four genes as internal control for qPCR was evaluated in mussel (Mytilus galloprovincialis) and clam (Ruditapes philippinarum) hemocytes after bacterial challenge. Four independent approaches (BestKeeper, GeNorm, NormFinder and DeltaCt ) were used to assess the suitable genes for stable expression. For these particular circumstances, the most stable gene in hemocytes was elongation factor 1 − α for mussels and α-tubulin for clams.

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“…Therefore, diseases can result in significant economic losses. The majority of diseases in clams are associated with Vibrio [1,2] and Perkinsus [3,4] species. Diseases can affect not only the development and survivorship of these organisms [5,6] but also the quality and price of the product.…”

Section: Introductionmentioning

confidence: 99%

Gene expression profile analysis of Manila clam (Ruditapes philippinarum) hemocytes after a Vibrio alginolyticus challenge using an immune-enriched oligo-microarray

Moreira

Milan

Balseiro

et al. 2013

Fish & Shellfish Immunology

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Experimental challenges of wild Manila clams with Perkinsus species isolated from naturally infected wild Manila clams

Cited by 44 publications

References 17 publications

Growth, mortality and susceptibility of oyster Crassostrea spp. to Perkinsus spp. infection during on growing in northeast Brazil

Growth, mortality and susceptibility of oyster Crassostrea spp. to Perkinsus spp. infection during on growing in northeast Brazil

Evaluation of reference genes ofMytilus galloprovincialisandRuditapes philippinaruminfected with three bacteria strains for gene expression analysis

Gene expression profile analysis of Manila clam (Ruditapes philippinarum) hemocytes after a Vibrio alginolyticus challenge using an immune-enriched oligo-microarray

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