Experimental Diabetes Upregulates the Expression of Uretereral Endothelin Receptors

Nakamura, Isao; Saito, Motoaki; Fukumoto, Yuji; Yoshida, Masaki; Nishi, Kazuhiko; Weiß, Robert M.; Latifpour, Jamshid

doi:10.1016/s0196-9781(97)00026-0

Cited by 11 publications

(12 citation statements)

References 11 publications

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“…Briefly, total RNA was quantitatively determined by the absorbance at 260 nm and 280 nm. Reverse transcription was carried out by the addition of M-MLV (Moloney murine leukemia virus)-reverse transcriptase (RT) and dNTPs at 37 • C for 105 min in a total reaction volume of 25 L. The reaction was terminated by 5 min incubation at 95 • C. The resulting RT products were stored at −20 • C (Nakamura et al, 1997). Amplification performed with the polymerase chain reaction (PCR) was carried out as follow.…”

Section: Rt-pcr Measurement Of Et a And Et B Receptor Expression In Tmentioning

confidence: 99%

Ethanol extracts of Rehmannia complex (Di Huang) containing no Corni fructus improve early diabetic nephropathy by combining suppression on the ET-ROS axis with modulate hypoglycemic effect in rats

Liu

Tang

Dai

et al. 2008

Journal of Ethnopharmacology

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Section: Rt-pcr Measurement Of Et a And Et B Receptor Expression In Tmentioning

confidence: 99%

Ethanol extracts of Rehmannia complex (Di Huang) containing no Corni fructus improve early diabetic nephropathy by combining suppression on the ET-ROS axis with modulate hypoglycemic effect in rats

Liu

Tang

Dai

et al. 2008

Journal of Ethnopharmacology

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“…Alterations in urinary [68,69], total renal [70,71], glomerular [72] and ureteral [73] ET-1 peptide, mRNA and receptor levels are present in animal models of diabetes. Studies with ET antagonists have also shown a role for this peptide in renal dysfunction in diabetes.…”

Section: In Vivo Haemodynamic Studiesmentioning

confidence: 99%

Endothelin: emerging role in diabetic vascular complications

1999

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The endothelin (ET) family consists of three 21-amino acid peptides designated ET-1, 2]. The biological actions of the ETs are primarily mediated by two distinct, G-protein-coupled receptor subtypes designated ET A and ET B [2]. Endothelin A receptors have a higher affinity for ET-1 and ET-2 than ET-3 and the ET B receptor binds all three isoforms with equal affinity. Translation of preproET mRNA generates preproET, which is converted to big ET and finally cleaved by ET converting enzyme (ECE) to facilitate production of biologically active peptide. Endothelin-1 released by vascular endothelial cells exerts an autocrine influence by promoting vasodilatation, subsequent to activation of ET B receptors located on endothelial cells. It also exerts a paracrine effect on adjacent vascular smooth muscle cells (VSMC) in evoking vasoconstrictor and mitogenic actions by activation of both ET A and ET B receptors. The primary target of ET-1 is the vasculature where it evokes transient vasodilatation mediated by endothelial ET B receptors, followed by slow-onset and sustained contraction mediated by ET A and ET B receptors located on VSMC. The functional response to ET-1 varies throughout different tissues and vascular beds due to differences in distribution and expression of these two receptor subtypes. Endothelin-1 Diabetologia (1999) AbstractSince the discovery of endothelin-1 as the most potent endothelial-derived vasoconstrictor/mitogenic peptide a decade ago, considerable evidence has implicated this peptide in various cardiovascular disease states, including diabetes mellitus. Plasma and tissue concentrations of endothelin-1 as well as responses to the peptide are changed in various forms of the disease in humans and animals. Endothelin activity is also altered in atherosclerotic and ischaemic disease, nephropathy, retinopathy, erectile dysfunction, and neuropathy, many of the well-known complications of diabetes. Striking new evidence shows that antagonists of the endothelin system might beneficially affect and potentially overcome some of these complications. Despite this, lack of direct proof of causation makes this peptide's role in the disease uncertain. This review examines the current state of thought on the role of endothelin in diabetes and in the complications of the disease as well as the likely roles of altered metabolic variables in modulating endothelin-1 concentrations and its activity. It is concluded that although alterations in endothelin-1 release and action are clearly associated with the diabetic state, further studies using inhibitors of the endothelin system are warranted to determine its precise role in the complications of the disease. [Diabetologia (1999

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“…This finding indicates that insulin treatment can Table 3 Table 3). 20 The similarity in the pharmacological profiles of rat ureteral ET receptors and cloned human ET A receptors and the differences in the pharmacological profiles of rat ureteral ET receptors and cloned human ET B receptors supports the predominance of the ET A receptor subtype in the rat ureter. …”

Section: Saturation Datamentioning

confidence: 66%

“…Our preliminary data show that sucrose-induced diuresis does not cause significant changes in the density of rat ureteral ET receptors compared with that in control animals. 20 Thus, excessive diuresis is not responsible for ET receptor up-regulation in the diabetic rat ureter. The up-regulation of ET receptors in the diabetic rat ureter may be caused by changes in protein synthesis and in diabetes-related physiological responses.…”

Section: Discussionmentioning

confidence: 94%

“…20 In inhibition binding studies, the ureteral membrane suspensions were diluted in the incubation buffer to approximately 400 volumes and stock mem-brane preparations of cloned human ET A or ET B receptors were diluted to 4-6 fmol/mL with the incubation buffer. 20 Aliquots of membrane particulates were incubated with a fixed concentration of [ 125 I]-ET-1 (approximately 20-25 pmol/L) and increasing concentrations of the following unlabelled peptides: ET-1 (non-selective), ET-3 (ET B/C selective), BQ 610 (ET A selective) and IRL 1620 (ET B selective) in a total volume of 0.25 mL at 23°C. 2,21,22 The inhibition assays were performed in duplicates.…”

Section: Inhibition Experimentsmentioning

confidence: 99%

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Effects of insulin treatment on diabetes‐induced alterations in endothelin receptors in rat ureter

1999

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Experimental Diabetes Upregulates the Expression of Uretereral Endothelin Receptors

Cited by 11 publications

References 11 publications

Ethanol extracts of Rehmannia complex (Di Huang) containing no Corni fructus improve early diabetic nephropathy by combining suppression on the ET-ROS axis with modulate hypoglycemic effect in rats

Ethanol extracts of Rehmannia complex (Di Huang) containing no Corni fructus improve early diabetic nephropathy by combining suppression on the ET-ROS axis with modulate hypoglycemic effect in rats

Endothelin: emerging role in diabetic vascular complications

Effects of insulin treatment on diabetes‐induced alterations in endothelin receptors in rat ureter

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