Experimental directional atherectomy injury in arterial vessels: Impact of trauma depth on cellular response

Gonschior, P.; Gerheuser, Florian; Gonschior, Gabriele-M.; Maier, Georg R.; Mack, Brigitte; Nerlich, Andreas G.; Lehr, Hans‐Anton; Höfling, B.

doi:10.1016/0002-8703(95)90385-2

Cited by 17 publications

(6 citation statements)

References 20 publications

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“…In addition, as most experimental studies have used oversized balloons or stent implantation as methods for vessel injury, one may argue that the pathogenesis of the vascular response following directional atherectomy may be somewhat different. However, the extensive proliferative response proportional to the severity of injury found in our experiments, and the described sequence of biological events following directional atherectomy in the porcine model, are similar to that described for other methods of porcine vascular injury [13].…”

Section: Study Limitationssupporting

confidence: 85%

“…Vessel injury was performed using the directional atherectomy catheter (AtheroCath GTO 7F, DVI, Inc. Temecula, CA) in the porcine model as previously described [13]. Briefly, the left coronary artery was selectively cannulated using a 9·5F guiding catheter (DVI, Inc., Temecula, CA).…”

Section: Methodsmentioning

confidence: 99%

“…Attending to the specific characteristics of the vessel wall damage induced by unidirectional atherectomy, depth and extension of damage was scored according to the following classification modified from Gonschior et al . [13] and Schwartz et al . [14]: score 0, no evidence of injury; score 1, intimal injury not affecting the internal elastic membrane (IEM); score 2: IEM and media damaged with less than 25% of the arterial circumference affected; score 3: IEM and media damaged with more than 25% of the circumference affected; score 4: adventitia damaged with less than 25% of the vessel affected; score 5: adventitia damaged with more than 25% of the circumference affected.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Effects of bosentan on neointimal response following coronary angioplasty

Sanmartı́n

Fernández‐Ortíz

Fantidis

et al. 2003

Eur J Clin Investigation

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Section: Study Limitationssupporting

confidence: 85%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of bosentan on neointimal response following coronary angioplasty

Sanmartı́n

Fernández‐Ortíz

Fantidis

et al. 2003

Eur J Clin Investigation

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“…32 Desmin has been found greatly diminished in vascular lesions such as in a pig model of directional atherectomy. 33 In addition, the levels of tropomyosin-1 were higher on the nanopatterned substrata relative to the unpatterned substrata (Fig. 3D).…”

Section: Analysis Of Vsmc Differentiation On Differentially Engineerementioning

confidence: 89%

Synergistic Effects of Matrix Nanotopography and Stiffness on Vascular Smooth Muscle Cell Function

Chaterji

Kim

Choe

et al. 2014

Tissue Engineering Part A

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Vascular smooth muscle cells (vSMCs) retain the ability to undergo modulation in their phenotypic continuum, ranging from a mature contractile state to a proliferative, secretory state. vSMC differentiation is modulated by a complex array of microenvironmental cues, which include the biochemical milieu of the cells and the architecture and stiffness of the extracellular matrix. In this study, we demonstrate that by using UV-assisted capillary force lithography (CFL) to engineer a polyurethane substratum of defined nanotopography and stiffness, we can facilitate the differentiation of cultured vSMCs, reduce their inflammatory signature, and potentially promote the optimal functioning of the vSMC contractile and cytoskeletal machinery. Specifically, we found that the combination of medial tissue-like stiffness (11 MPa) and anisotropic nanotopography (ridge width_groove width_ridge height of 800_800_600 nm) resulted in significant upregulation of calponin, desmin, and smoothelin, in addition to the downregulation of intercellular adhesion molecule-1, tissue factor, interleukin-6, and monocyte chemoattractant protein-1. Further, our results allude to the mechanistic role of the RhoA/ROCK pathway and caveolin-1 in altered cellular mechanotransduction pathways via differential matrix nanotopography and stiffness. Notably, the nanopatterning of the stiffer substrata (1.1 GPa) resulted in the significant upregulation of RhoA, ROCK1, and ROCK2. This indicates that nanopatterning an 800_800_600 nm pattern on a stiff substratum may trigger the mechanical plasticity of vSMCs resulting in a hypercontractile vSMC phenotype, as observed in diabetes or hypertension. Given that matrix stiffness is an independent risk factor for cardiovascular disease and that CFL can create different matrix nanotopographic patterns with high pattern fidelity, we are poised to create a combinatorial library of arterial test beds, whether they are healthy, diseased, injured, or aged. Such high-throughput testing environments will pave the way for the evolution of the next generation of vascular scaffolds that can effectively crosstalk with the scaffold microenvironment and result in improved clinical outcomes.

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“…Using an atherectomy device, Gonshior et al. created a comparable arterial injury on porcine femoral arteries leading to significant IH after 21 days (Gonschior et al. 1995).…”

Section: Discussionmentioning

confidence: 99%

A novel model of accelerated intimal hyperplasia in the pig iliac artery

Houbballah

Robaldo

Albadawi

et al. 2011

International Journal of Experimental Pathology

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There is no good animal model of large artery injury-induced intimal hyperplasia (IH). Those available are reproducible, providing only a few layers of proliferating cells or have the disadvantage of the presence of a metallic stent that complicates histology evaluation. This study was designed to develop a new, simple model of accelerated IH based on balloon injury in conjunction with disruption of the Internal Elastic Lamina (IEL) in pig external iliac arteries. Iliac artery injury (n = 24) was performed in 12 Yorkshire pigs divided in two groups: Group I (n = 10), overdistention injury induced by an oversized non-compliant balloon; Group II (n = 14), arterial wall disruption by pulling back an isometric cutting balloon (CB) followed by stretching with a compliant Fogarty Balloon (FB). At two weeks, arteries were processed for morphometric analysis and immunohistochemistry (IHC) for smooth muscle cells (SMC) and proliferating cell nuclear antigen (PCNA). When comparing the two groups, at 2 weeks, arteries of group II had a higher incidence of IH (100%vs. 50%, P = 0.0059), increased intimal areas (2.54 ± 0.33 mm(2) vs. 0.93 ± 0.36 mm(2) , P = 0.004), increased intimal area/Media area ratios (0.95 ± 0.1 vs. 0.28 ± 0.05; P < 0.0001) and decreased lumen areas (6.24 ± 0.44 vs. 9.48 ± 1.56, P = 0.026). No thrombosis was noticed in Group II. Neointima was composed by proliferating SMC located with the highest concentration in the area of IEL disruption (IHC). Arterial injury by pulling back CB and FB induces significant IH in pig iliac arteries by two weeks without thrombosis. This model is superior to the classical overdistention non-compliant model and should be useful and cost-effective for preclinical testing of procedures designed to inhibit IH in large peripheral arteries.

show abstract

Experimental directional atherectomy injury in arterial vessels: Impact of trauma depth on cellular response

Cited by 17 publications

References 20 publications

Effects of bosentan on neointimal response following coronary angioplasty

Effects of bosentan on neointimal response following coronary angioplasty

Synergistic Effects of Matrix Nanotopography and Stiffness on Vascular Smooth Muscle Cell Function

A novel model of accelerated intimal hyperplasia in the pig iliac artery

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