Experimental Dual Infection of Cesarean-Derived, Colostrum-Deprived Pigs with Mycoplasma hyopneumoniae and Pseudorabies Virus.

Shibata, Isao; Okada, Mizue; Uruno, Katsuyoshi; Samegai, Yasuo; Ono, Masaaki; Sakano, Tetsuya; Sato, Shizuo

doi:10.1292/jvms.60.295

Cited by 23 publications

(14 citation statements)

References 20 publications

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“…33 The interaction between M. hyopneumoniae and other respiratory pathogens has been previously studied, and many that pneumonia caused by mixed respiratory infections has a more severe clinical course. 1,4,6,28,35,36,39 By numerically expressing the CT lung lesions as average density values, a significant difference was clearly demonstrated between the M. hyopneumoniae-infected and uninfected pigs, but not between FB 1 -exposed and unexposed pigs. The absence of a significant increase in the average lung density value in the pigs exposed to both FB 1 and M. hyopneumoniae infection could be due to the low numbers of experimental animals or, in part, because 1 MF group pig with the most severe lung lesions was euthanized before the end of study and its average lung density value was not included in the statistical analysis.…”

Section: Discussionmentioning

confidence: 99%

Use of Computed Tomography and Histopathologic Review for Lung Lesions Produced by the Interaction BetweenMycoplasma hyopneumoniaeand Fumonisin Mycotoxins in Pigs

et al. 2013

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Mycoplasma hyopneumoniae has a primary role in the porcine respiratory disease complex (PRDC). The objective of this study was to determine whether fumonisin mycotoxins influence the character and/or the severity of pathological processes induced in the lungs of pigs by Mycoplasma hyopneumoniae. Four groups of pigs (n ¼ 7/group) were used, one fed 20 ppm fumonisin B 1 (FB 1 ) from 16 days of age (group F), one only infected with M. hyopneumoniae on study day 30 (group M), and a group fed FB 1 and infected with M. hyopneumoniae (group MF), along with an untreated control group (group C). Computed tomography (CT) scans of infected pigs (M and MF) on study day 44 demonstrated lesions extending to the cranial and middle or in the cranial third of the caudal lobe of the lungs. The CT images obtained on study day 58 showed similar but milder lesions in 5 animals from group M, whereas lungs from 2 pigs in group MF appeared progressively worse. The evolution of average pulmonary density calculated from combined pixel frequency values, as measured by quantitative CT, was significantly influenced by the treatment and the age of the animals. The most characteristic histopathologic lesion in FB 1 -treated pigs was pulmonary edema, whereas the pathomorphological changes in Mycoplasma-infected pigs were consistent with catarrhal bronchointerstitial pneumonia. FB 1 aggravated the progression of infection, as demonstrated by severe illness requiring euthanasia observed in 1 pig and evidence of progressive pathology in 2 pigs (group MF) between study days 44 and 58.

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Section: Discussionmentioning

confidence: 99%

Use of Computed Tomography and Histopathologic Review for Lung Lesions Produced by the Interaction BetweenMycoplasma hyopneumoniaeand Fumonisin Mycotoxins in Pigs

et al. 2013

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“…Mycoplasma culture: Mycoplasma culture was performed in a BHL medium as described previously [17]. Samples were diluted in a series of 10 fold-dilutions with medium and incubated at 37°C for up to 4 weeks.…”

Section: Methodsmentioning

confidence: 99%

Detection of Mycoplasma hyopneumoniae in Lung and Nasal Swab Samples from Pigs by Nested PCR and Culture Methods

et al. 2005

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ABSTRACT. We examined nasal swab and lung homogenate samples collected from pigs experimentally and naturally infected with Mycoplasma hyopneumoniae for the detection of M. hyopneumoniae by the nested PCR (nPCR) and culture methods. In the 23 experimentally infected pigs, M. hyopneumoniae was commonly detected in nasal swabs by the nPCR and culture methods at 4 weeks after inoculation, and there was a significant correlation (P<0.01) between the titers of viable organisms in nasal swabs and in lung homogenates in the experimentally inoculated pigs. In the naturally infected pigs, on the other hand, discrepancies in detection were found between nasal swab and lung homogenate samples in 17 of 36 cases, although the presence of gross lung lesions correlated relatively well with the detection of organisms from the samples. Our results indicated that the diagnosis of mycoplasmal pneumonia by nPCR in individual pigs with nasal swabs is reliable under these experimental conditions. At present, nPCR with nasal swabs should only be used for monitoring the disease status at the herd level under field conditions.

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“…Isolation of organisms: Mycoplasma cultures were performed from samples with lung lesions, which were homogenated to make a 10% suspension (w/v) in a BHL medium [21] as previously described [16]. Cultures were identified as M. hyopneumoniae by a metabolism inhibition test [19].…”

Section: Mycoplasmal Pneumonia Of Swine (Mps) Caused Bymentioning

confidence: 99%

Evaluation of Mycoplasma hyopneumoniae Inactivated Vaccine in Pigs under Field Conditions.

Okada

Sakano

Senna³

et al. 1999

J. Vet. Med. Sci.

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ABSTRACT. An inactivated vaccine prepared from broth culture supernatant of Mycoplasma hyopneumoniae with an aluminum adjuvant was evaluated in three herds (herd A: specific pathogen-free herd, herd B: high health status herd with no clinical signs of respiratory infection, herd C: low health status herd with serious epidemiological and economical problems). A total of 212 pigs from the three herds were divided into two groups. One group was injected twice with the vaccine at 4-week intervals and the other was a control group. No adverse reactions were noted following the vaccinations either systematically or locally in any of the vaccinated pigs from any of the herds. In herd A, the vaccination provided antibody response within 4 weeks after the second vaccination and antibody responses continued for more than 12 weeks. In herds B and C, the number of pigs with lung lesions, mean percentage of lung lesions, and the numbers of M. hyopneumoniae recovered from pigs at slaughter in the vaccinated group were significantly (P<0.05) reduced compared to the control group. Furthermore, vaccination resulted in improved average daily weight gain (ADG), improved feed conversion ratio (FCR), and improved days to market weight in herd C, whereas no difference in growth performance was shown in herd B. It is suggested that the inactivated vaccine prepared from broth culture supernatant of M. hyopneumoniae is effective in reducing clinical signs and lung lesions. Also, vaccination resulted in improved growth performance in herds where clinical signs and economic losses were significant.-KEY WORDS: field trial, Mycoplasma hyopneumoniae, mycoplasmal pneumonia of swine, swine, vaccine.

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Experimental Dual Infection of Cesarean-Derived, Colostrum-Deprived Pigs with Mycoplasma hyopneumoniae and Pseudorabies Virus.

Cited by 23 publications

References 20 publications

Use of Computed Tomography and Histopathologic Review for Lung Lesions Produced by the Interaction BetweenMycoplasma hyopneumoniaeand Fumonisin Mycotoxins in Pigs

Use of Computed Tomography and Histopathologic Review for Lung Lesions Produced by the Interaction BetweenMycoplasma hyopneumoniaeand Fumonisin Mycotoxins in Pigs

Detection of Mycoplasma hyopneumoniae in Lung and Nasal Swab Samples from Pigs by Nested PCR and Culture Methods

Evaluation of Mycoplasma hyopneumoniae Inactivated Vaccine in Pigs under Field Conditions.

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