Experimental evaluation of the efficiency of chitosan matrixes under conditions of modeling of bone defect in vivo (preliminary message)

Abstract

Background. Despite the wide range of studies, the development of osteoplastic material, which has not only osteoconductive but also osteoinductive properties, remains an extremely topical issue in modern medical materials science. This work is devoted to experimental evaluation of the effectiveness of synthetic osteoplastic composite material based on chitosan and hydroxyapatite.
 Aim. This study aimed to determine the effects of spongy implants based on chitosan and its composite with hydroxyapatite nanoparticles in an amount of 50 wt. % on early osteogenesis in the area of the through defect of the ileum.
 Materials and methods. The studied materials were sponge implants based on chitosan and its composite with hydroxyapatite nanoparticles in an amount of 50 wt. %. Comparison groups include those without implant placement and those with replacement with commercial Reprobone osteoplastic material. Materials were implanted into the zone of the through defect of the ileum of rabbits for a period of 28 days.
 Results. A high rate of resorption of materials based on chitosan in bone tissue and active growth of reticulofibrotic bone tissue along the edges of the defect was established, and the formation of cartilaginous islands and bone marrow was recorded in the group of chitosan implants with hydroxyapatite. The aseptic effect was observed with the use of implants made of chitosan and hydroxyapatite.
 Conclusions. The data obtained allow us to argue about the osteoconductivity of the studied materials and the prospects for further development in this direction.

Morphological Reconstruction of a Critical-Sized Bone Defect in the Maxillofacial Region Using Modified Chitosan in Rats with Sub-Compensated Type I Diabetes Mellitus

“…The design, which contains high-purity and deacetylated chitosan, sodium chondroitin sulfate, sodium hyaluronate, heparin sulfate, sodium alginate and amorphous hydroxyapatite in certain weight ratios, is designed for early stimulation of bone formation. It is known that a nanosized surface, in comparison with a micro-sized one, is a stimulus for the proliferation of endothelial cells and osteogenic cells [38]. It is important to note that a high degree of deacetylation of the chitosan matrix plays an important role both in terms of structure degradation and in the degree of its endothelization [67].…”

“…Nano-sized hydroxyapatite, which ensures the strength of bone tissue, is located between bundles of collagen fibers in the form of parallel-oriented plates and increases the synthesis of its own bone tissue. The inclusion of hydroxyapatite in the chitosan matrix is designed not only to enhance the strength characteristics of the implant, but also for the early formation of native bone [38].…”

Development and IR spectroscopic Analysis of Composite Materials Based on Poly(Methyl Methacrylate) and Chitosan

“…Chitosan is used for obtaining materials used in arthroplasty for filling bone defects, for creating nanosystems in drug delivery, for modifying synthetic prostheses of blood vessels [6][7][8][9]. Chitosan has immunomodulating, antitumoral, antioxidative, hemostatic, bacteriostatic and fungistatic properties, high bio-compatibility with human tissues, and high sorption properties [10][11][12][13][14][15].…”