Experimental evidence for white spot syndrome virus (<scp>WSSV</scp>) susceptibility linked to a microsatellite <scp>DNA</scp> marker in <scp>g</scp>iant <scp>b</scp>lack <scp>t</scp>iger <scp>s</scp>hrimp, <i><scp>P</scp>enaeus monodon</i> (Fabricius)

Dutta, Sourav; Chakrabarty, Usri; Mallik, Ajoy; Mandal, Nripendranath

doi:10.1111/jfd.12006

Cited by 14 publications

(15 citation statements)

References 13 publications

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“…Previously, a 71-bp microsatellite DNA marker was reported to identify diseaseresistant and disease-susceptible individuals (Mukherjee & Mandai, 2009), and later this phenomenon was confirmed by a WSSV challenge experiment (Dutta et al, 2013a). The present study shows the varying disease-resistant prevalence (31.4% in 2009 and 37.1% in 2010) among various cultured ponds in both years.…”

Section: Discussionsupporting

confidence: 66%

“…Specific pathogen resistant (SPR) broodstock generation will be one of the most important strategies for the development of a disease-free shrimp aquaculture industry. Therefore, if farmers are able to use only disease-resistant seeds instead of genetically unselected seeds for their aquaculture industry, it might prevent huge economic destruction due to viral diseases (Dutta et al, 2013b;Chakrabarty et ah, 2014;Mallik et al, 2014). The present study will be very relevant to screen for WSSV and diseaseresistant prevalence among commercially cultured populations of P. monodon.…”

Section: Discussionmentioning

confidence: 92%

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White spot syndrome virus (WSSV) and prevalence of disease resistance in a commercially cultured population of Penaeus monodon Fabricius, 1798 (Decapoda, Dendrobranchiata)

Chakrabarty

Dutta

Mallik

et al. 2014

Crustac

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White spot disease (WSD) caused by the white spot syndrome virus (WSSV) is the leading cause for huge economic destruction in the shrimp aquaculture industry. The present study investigates the prevalence of WSSV and disease-resistant shrimps among a commercially cultured population of Penaeus monodon Fabricius, 1798. Results suggest that WSSV prevalence among 32 culture ponds affected by various diseases was 58.2% cumulatively in 2009 and 2010. Real-time PCR yielded 1.46 x 108, 1.94 x 106 and 3.6 x 104 copies of WSSV per fig of genomic DNA of shrimp in severe, moderate and low infected shrimps, successively. Among the shrimps collected, the prevalence of disease resistance was 31.4% and 37.1% consecutively in 2009 and 2010. It was found that 34% of the shrimps were disease resistant while considering both years. This study shows the importance of using disease-resistant seeds for better shrimp health management. RÉSUMÉLa maladie des points blancs (WSD) causée par le virus du syndrome des points blancs (WSSV) est la principale cause d'importantes pertes économiques dans l'industrie aquacole des crevettes. Cette étude examine la prévalence de WSSV et les crevettes résitantes à la maladie dans les populations d'aquaculture de Penaeus monodon Fabricius, 1798. Les résultats suggèrent que la prévalance de WSSV parmi les 32 bassins de culture affectés par des maladies variées a été pour 2009 et 2010 en moyenne de 58,2%. Le montant de WSSV mesuré par PCR en temps réel a été chez les crevettes infectées sévèrement, moyennement ou peu, successivement de 1,46 x 108, 1,94 x 106 et 3,6 x 104 copies de WSSV fig~1 de DNA génomique de crevette. Parmi les crevettes collectées, la prévalence de la résistance à la maladie a été de 31,4% en 2009 et 37,1% en 2010. Il a été trouvé que 34% des crevettes étaient résistantes en considérant les deux années. Cette étude souligne l'importance d'utiliser des souches résistant à la maladie pour une meilleure gestion de la santé des crevettes.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 92%

White spot syndrome virus (WSSV) and prevalence of disease resistance in a commercially cultured population of Penaeus monodon Fabricius, 1798 (Decapoda, Dendrobranchiata)

Chakrabarty

Dutta

Mallik

et al. 2014

Crustac

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“…This implies that there may be a potential genetic marker for WSSV susceptibility in G 8 and G 8iA . In line with this result, Dutta et al (2013) reported that WSSV susceptibility and WSSV resistance of P. monodon in an Indian population linked to a microsatellite 71 bp DNA marker. Hence, we speculate that PmVRP15 is not universally associated with WSSV susceptibility in other populations besides the Thai population.…”

Section: Fig 2 Cumulative Mortality Of P Monodon Fabricius In G 8 Asupporting

confidence: 62%

Limited evidence for white spot syndrome virus susceptibility associated with expression of PmVRP15 in local population of giant tiger shrimp (Penaeus monodon)

et al. 2017

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White spot syndrome virus (WSSV) is a devastating viral disease in shrimp aquaculture. Infection of WSSV in penaeid shrimps affects immune defense and changes gene expression. PmVRP15 has been reported as a part of the WSSV propagation pathway that is highly up-regulated in hemocytes at the acute phase of WSSV infection. This study analyzed the expression of PmVRP15 in local populations of giant tiger shrimp (Penaeus monodon) to be associated with susceptibility to WSSV. Tested populations consisted of an inbreeding population (G 8 ) and outbreeding population (G 8iA ) from Jepara, Indonesia. Susceptibility was determined by cumulative mortality, median lethal time (LT 50 ), and severity of infection at time of death. Though all populations were susceptible to WSSV, the first mortality in G 8 occurred at 18 hours post-infection (hpi) with mild infection, while first mortality of G 8iA occurred at 30 hpi with severe infection. The LT 50 of G 8 was significantly lower than that of G 8iA , indicating that G 8iA was less susceptible to WSSV than G 8 . Relative PmVRP15 transcripts of G 8iA were insignificantly down-regulated, whereas relative PmVRP15 transcripts of G 8 were insignificantly upregulated. Although it's still not conclusive, the results of this study suggest that PmVRP15 has weak potential as a WSSV susceptibility marker in G 8 and G 8iA broodstock selection.

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“…This result is consistent with our earlier finding where WSSV propagation is also 1.21 × 10 3 fold higher in WSSV‐exposed disease susceptible shrimps selected by another 71 bp microsatellite DNA marker (Dutta et al . ). However, it is important to develop more disease resistant DNA markers associated with QTL(s) that will give a clearer idea whether WSSV resistance trait in P. monodon is polygenic or monogenic.…”

Section: Discussionmentioning

confidence: 97%

“…Until now, a 71 bp microsatellite DNA marker has been developed to identify disease resistant populations of P. monodon (Mukherjee & Mandal ), and this phenomenon was further confirmed by WSSV challenge experiment (Dutta et al . ).…”

Section: Introductionmentioning

confidence: 97%

Identification of RAPD‐SCAR marker linked to white spot syndrome virus resistance in populations of giant black tiger shrimp, Penaeus monodon Fabricius

Dutta

Biswas

Mukherjee

et al. 2013

Journal of Fish Diseases

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White spot disease (WSD) caused by white spot syndrome virus (WSSV) creates severe epizootics in shrimp aquaculture industry worldwide. Despite several efforts, no such permanent remedy was yet developed. Selective breeding using DNA markers would be a cost-effective strategy for long-term solution of this problem. In the present investigation, out of 30 random primers, only one primer produced a statistically significant (P < 0.01) randomly amplified polymorphic DNA (RAPD) marker of 502 bp, which provided a good discrimination between disease resistant and disease susceptible populations of Penaeus monodon from three geographical locations along the East coast of India. Because RAPD markers are dominant, a sequence characterized amplified region (SCAR) marker was developed by cloning and sequencing of 502 bp RAPD fragment, which generates a single 457 bp DNA fragment after PCR amplification only in the disease resistant shrimps. Challenge experiment was also conducted to validate this 457 bp SCAR marker, and the results suggested that the WSSV loads were 2.25 × 10(3) fold higher in disease susceptible than that in disease resistant shrimps using real-time PCR. Therefore, this 457 bp DNA SCAR marker will be very valuable towards the development of disease-free shrimp aquaculture industry.

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Experimental evidence for white spot syndrome virus (WSSV) susceptibility linked to a microsatellite DNA marker in giant black tiger shrimp, Penaeus monodon (Fabricius)

Cited by 14 publications

References 13 publications

White spot syndrome virus (WSSV) and prevalence of disease resistance in a commercially cultured population of Penaeus monodon Fabricius, 1798 (Decapoda, Dendrobranchiata)

White spot syndrome virus (WSSV) and prevalence of disease resistance in a commercially cultured population of Penaeus monodon Fabricius, 1798 (Decapoda, Dendrobranchiata)

Limited evidence for white spot syndrome virus susceptibility associated with expression of PmVRP15 in local population of giant tiger shrimp (Penaeus monodon)

Identification of RAPD‐SCAR marker linked to white spot syndrome virus resistance in populations of giant black tiger shrimp, Penaeus monodon Fabricius

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