Experimental evidence of the kinetic performance achievable with columns packed with new 1.9μm fully porous particles of narrow particle size distribution

Ismail, Omar H.; Catani, Martina; Pasti, Luisa; Cavazzini, Alberto; Ciogli, Alessia; Villani, Claudio; Kotoni, Dorina; Gasparrini, Francesco; Bell, David S.

doi:10.1016/j.chroma.2016.05.038

Cited by 36 publications

(42 citation statements)

References 35 publications

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“…UHPLC conditions are very suitable in 2 D, increasing speed and resolution, as shown in a consistent number of applications such as polymers, pharmaceuticals, carotenoids and peptides [23-25, [18]]. In this work we investigated the performance in the 2 D of a short (50 × 3.0 mm, L. × I.D) sub-2 μm fully porous monodisperse Titan TM C-18 column, recently introduced in the market and characterized in detail by Ismail et al [17]. As reported in our previous work [18], this column maintains its efficiency at high linear velocities; this aspect makes it an excellent candidate for 2 D of LC × LC where ultra-fast separations are mandatory.…”

Section: Preliminary Search Of Chromatographic Condition In Both Dimementioning

confidence: 99%

Development of an improved online comprehensive hydrophilic interaction chromatography × reversed-phase ultra-high-pressure liquid chromatography platform for complex multiclass polyphenolic sample analysis

et al. 2017

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In this study, an improved online comprehensive two-dimensional liquid chromatography platform coupled to tandem mass spectrometry was developed for the analysis of complex polyphenolic samples. A narrowbore hydrophilic interaction chromatography column (150 × 2.0 mm, 3.0 μm, cross-linked diol) was employed in the first dimension, while a reversed-phase column based on monodisperse sub-2 μm fully porous particles (50 × 3.0 mm, 1.9 μm d.p.) with high surface area (410 m /g) was employed in the second dimension. The combination of a trapping column modulation interface with the high retentive fully porous monodisperse reversed-phase column in the second dimension resulted in higher peak capacity values (1146 versus 867), increased sensitivity, sharper and more symmetrical peaks in comparison with a conventional loop-based method, with the same analysis time (70 min). The system was challenged against a complex polyphenolic extract of a typical Italian apple cultivar, enabling the simultaneous separation of multiple polyphenolic classes, including oligomeric procyanidins, up to degree of polymerization of 10. Hyphenation with an ion trap time-of-flight mass spectrometer led to the tentative identification of 121 analytes, showing how this platform could be a powerful analytical tool for the accurate profiling of complex polyphenolic samples.

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Section: Preliminary Search Of Chromatographic Condition In Both Dimementioning

confidence: 99%

Development of an improved online comprehensive hydrophilic interaction chromatography × reversed-phase ultra-high-pressure liquid chromatography platform for complex multiclass polyphenolic sample analysis

et al. 2017

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“…From these plots, one can easily notice that the values of

a (ν)

of the FPP‐1.9 column (red squares) are unexpectedly smaller than those of the SPP‐2.0 column (blue triangles) in agreement, however, with Refs. 35 and 36. Small values of eddy dispersion come from efficient packing of columns.…”

Section: Resultsmentioning

confidence: 99%

“…Some of the author of this work recently explored the kinetic performance of two columns packed with sub‐2 μm nPSD C18 FPPs with different pore size. Results of that investigation revealed an extremely low eddy dispersion in both cases [35, 36].…”

Section: Introductionmentioning

confidence: 99%

Investigation of mass transfer properties and kinetic performance of high‐efficiency columns packed with C₁₈ sub‐2 μm fully and superficially porous particles

Felletti

Luca

Lievore

et al. 2020

J of Separation Science

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Three columns packed with 2.0 μm superficially porous particles, 1.7 μm fully porous particles, and monodisperse 1.9 μm fully porous particles with narrow particle size distribution have been deeply characterized from a kinetic point of view. The 1.9 μm column showed excellent kinetic performance, comparable to that of the superficially porous one. These two columns also exhibit flatter c-branches of the van Deemter curve compared to the 1.7 μm fully porous particles column, resulting in smaller loss of efficiency when they are operated at higher flow rates than the optimal ones. The independent evaluation of each contribution to band broadening has revealed that the difference in kinetic performance comes from the very small eddy dispersion contribution on the 1.9 μm column, surprisingly even lower than that of the superficially porous one. This finding suggests a very good packing of the monodisperse 1.9 μm column. On the other hand, the potential of 1.7 μm fully porous particles is completely broken down by the strong frictional heating effect already arising at relatively low flow rates. K E Y W O R D Sfully porous particles, kinetic performance, octadecyl stationary phase, superficially porous particles, ultrahigh-performance liquid chromatography

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“…There is an ongoing debate whether the improved column packing is related to a narrower particle size distribution (Destefano et al, 2008) or to higher surface roughness of the particles. Recently Desmet Ismail et al, 2016b) and Gritti and Guiochon (2015a,b) have reported on fully porous particles with a very narrow particle size distribution (Titan-C18) that also shows reduced plate heights well below 2.…”

Section: Coreeshell Particles 193mentioning

confidence: 99%

Theory and Practice of UHPLC and UHPLC–MS

Guillarme

Veuthey

2017

Handbook of Advanced Chromatography /Mass Spectrometry Techniques

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Experimental evidence of the kinetic performance achievable with columns packed with new 1.9μm fully porous particles of narrow particle size distribution

Cited by 36 publications

References 35 publications

Development of an improved online comprehensive hydrophilic interaction chromatography × reversed-phase ultra-high-pressure liquid chromatography platform for complex multiclass polyphenolic sample analysis

Development of an improved online comprehensive hydrophilic interaction chromatography × reversed-phase ultra-high-pressure liquid chromatography platform for complex multiclass polyphenolic sample analysis

Investigation of mass transfer properties and kinetic performance of high‐efficiency columns packed with C₁₈ sub‐2 μm fully and superficially porous particles

Theory and Practice of UHPLC and UHPLC–MS

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Experimental evidence of the kinetic performance achievable with columns packed with new 1.9μm fully porous particles of narrow particle size distribution

Cited by 36 publications

References 35 publications

Development of an improved online comprehensive hydrophilic interaction chromatography × reversed-phase ultra-high-pressure liquid chromatography platform for complex multiclass polyphenolic sample analysis

Development of an improved online comprehensive hydrophilic interaction chromatography × reversed-phase ultra-high-pressure liquid chromatography platform for complex multiclass polyphenolic sample analysis

Investigation of mass transfer properties and kinetic performance of high‐efficiency columns packed with C18 sub‐2 μm fully and superficially porous particles

Theory and Practice of UHPLC and UHPLC–MS

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Investigation of mass transfer properties and kinetic performance of high‐efficiency columns packed with C₁₈ sub‐2 μm fully and superficially porous particles