Experimental exposure of blue mussels ( Mytilus galloprovincialis ) to high levels of benzo[ a ]pyrene and possible implications for human health

Speciale, Antonio; Zena, R.; Calabrò, Concetta; Bertuccio, Clara; Aragona, Marialuisa; Saija, A.; Trombetta, Domenico; Cimino, Francesco; Cascio, Patrizia Lo

doi:10.1016/j.ecoenv.2017.12.038

Cited by 33 publications

(14 citation statements)

References 52 publications

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“…Similarly, in mussels M. galloprovincialis from the petrochemical polluted site, severe morphological alterations were also observed in gills 29 . Speciale et al 55 exerted experimental exposure of M. galloprovincialis to 0.5 and 1 mg/L Bap, and found that high levels of Bap exposure resulted in loss of structural integrity characterized by detachment and degeneration of epithelial cells, sloughing of epithelium and denuded of cilia. Additionally, the severity of the damage was dependent on Bap concentration and exposure duration 55 .…”

Section: Discussionmentioning

confidence: 99%

“…Speciale et al 55 exerted experimental exposure of M. galloprovincialis to 0.5 and 1 mg/L Bap, and found that high levels of Bap exposure resulted in loss of structural integrity characterized by detachment and degeneration of epithelial cells, sloughing of epithelium and denuded of cilia. Additionally, the severity of the damage was dependent on Bap concentration and exposure duration 55 . These findings collectively indicated that petrochemical pollutants containing Bap could induce high oxidative stress to bivalve molluscs and lead to morphological alterations in organ tissues for instance in gills.…”

Section: Discussionmentioning

confidence: 99%

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Acute benzo[a]pyrene exposure induced oxidative stress, neurotoxicity and epigenetic change in blood clam Tegillarca granosa

Guo

Feng

et al. 2021

Sci Rep

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The blood clam (Tegillarca granosa) is being developed into a model bivalve mollusc for assessing and monitoring marine pollution on the offshore seabed. However, the information on the response of blood clam to PAHs, an organic pollutant usually deposited in submarine sediment, remains limited. Herein, we employed multiple biomarkers, including histological changes, oxidative stress, neurotoxicity and global DNA methylation, to investigate the effects of 10 and 100 μg/L Bap exposure on the blood clams under laboratory conditions, as well as the potential mechanisms. Acute Bap exposure can induce significant morphological abnormalities in gills as shown through hematoxylin–eosin (H.E) staining, providing an intuitive understanding on the effects of Bap on the structural organization of the blood clams. Meanwhile, the oxidative stress was significantly elevated as manifested by the increase of antioxidants activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and glutathione-s-transferase (GST), lipid peroxidation (LPO) level and 8-hydroxy-2′-deoxyguanosine (8-OHdG) content. The neurotoxicity was also strengthened by Bap toxicity manifested as inhibited acetylcholinesterase (AChE) and choline acetyltransferase (ChAT) activities. In addition, the global DNA methylation level was investigated, and a significant DNA hypomethylation was observed in Bap exposed the blood clam. The correlation analysis showed that the global DNA methylation was negatively correlated with antioxidants (SOD, CAT and POD) activities, but positively correlated choline enzymes (AChE and ChAT) activities. These results collectively suggested that acute Bap exposure can cause damage in gills structures in the blood clam possibly by generating oxidative stress and neurotoxicity, and the global DNA methylation was inhibited to increase the transcriptional expression level of antioxidants genes and consequently elevate antioxidants activities against Bap toxicity. These results are hoped to shed some new light on the study of ecotoxicology effect of PAHs on marine bivalves.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Acute benzo[a]pyrene exposure induced oxidative stress, neurotoxicity and epigenetic change in blood clam Tegillarca granosa

Guo

Feng

et al. 2021

Sci Rep

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“…BaP reacts and binds to DNA, making it a highly efficient mutagen and carcinogen [41]. It is the only PAH classified as a recognized carcinogen by the International Agency for Research on Cancer (IARC) [42] and has routinely been employed as model contaminant for this group. It has also been classified as a candidate for being a substance "of very high concern" in the Registration, Evaluation, Authorization, and Restriction of Chemicals (REACH).…”

Section: Introductionmentioning

confidence: 99%

“…It has also been classified as a candidate for being a substance "of very high concern" in the Registration, Evaluation, Authorization, and Restriction of Chemicals (REACH). Exposure has been found to induce CYP1A and morphological changes in gill tissue [42], as well as being found to accumulate through the food chain [43]. Direct exposure of mussels M. galloprovincialis to 20-25 µm low density PE (LDPE) MPs led to particle localization in the haemolymph and gills, as well as digestive tissue, whilst BaP contaminated MPs led to significant alterations of the immune system [44].…”

Section: Introductionmentioning

confidence: 99%

Accumulation, Depuration, and Biological Effects of Polystyrene Microplastic Spheres and Adsorbed Cadmium and Benzo(a)pyrene on the Mussel Mytilus galloprovincialis

Hellfeld

Zarzuelo

Zaldibar

et al. 2022

Toxics

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Filter feeders are target species for microplastic (MP) pollution, as particles can accumulate in the digestive system, disturbing feeding processes and becoming internalized in tissues. MPs may also carry pathogens or pollutants present in the environment. This work assessed the influence of polystyrene (PS) MP size and concentration on accumulation and depuration time and the role of MPs as vectors for metallic (Cd) and organic (benzo(a)pyrene, BaP) pollutants. One-day exposure to pristine MPs induced a concentration-dependent accumulation in the digestive gland (in the stomach and duct lumen), and after 3-day depuration, 45 µm MPs appeared between gill filaments, while 4.5 µm MPs also occurred within gill filaments. After 3-day exposure to contaminated 4.5 µm MPs, mussels showed increased BaP levels whilst Cd accumulation did not occur. Here, PS showed higher affinity to BaP than to Cd. Three-day exposure to pristine or contaminated MPs did not provoke significant alterations in antioxidant and peroxisomal enzyme activities in the gills and digestive gland nor in lysosomal membrane stability. Exposure to dissolved contaminants and to MP-BaP caused histological alterations in the digestive gland. In conclusion, these short-term studies suggest that MPs are ingested and internalized in a size-dependent manner and act as carriers of the persistent organic pollutant BaP.

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“…Benzopyrene (BP) is a PAH found in tobacco smoke, coal tar, grilled meats, and many foods. BP, one of the most intensively studied pollutants, has been considered a potentially carcinogenic substance and an important risk factor for cardiovascular diseases [5, 6]. In addition, environmental pollution, such as BP from cigarette smoking, has momentous damages on the stemness and proliferation of various stem cells [7–9].…”

Section: Introductionmentioning

confidence: 99%

Environmental Benzopyrene Attenuates Stemness of Placenta-Derived Mesenchymal Stem Cells via Aryl Hydrocarbon Receptor

Heo

Lim

Pyo

et al. 2019

Stem Cells International

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The toxic effects of particulate matter have been linked to polycyclic aromatic hydrocarbons (PAHs) such as benzopyrene. PAHs are potent inducers of the aryl hydrocarbon receptor (AhR), which is an expressed nuclear receptor that senses environmental stimuli and modulates gene expression. Even though several studies have shown that the benzopyrene (BP) of chemical pollutants significantly impaired stem cell activity, the exact molecular mechanisms were not clearly elucidated. In the present study, we aimed to investigate the effects of BP on placenta-derived mesenchymal stem cells (PD-MSCs) in vitro. We found that the AhR in PD-MSCs was expressed under the treatment of BP, and its activation markedly disrupted osteogenic differentiation through the alteration of stemness activity of PD-MSCs. Moreover, BP treatment significantly reduced the proliferation activity of PD-MSCs and expression of pluripotent markers through the induction of AhR. Treatment with StemRegenin 1 (SR1), a purine derivative that antagonizes the AhR, effectively prevented BP-induced reduction of the proliferation and differentiation activity of PD-MSCs. In this study, we found that BP treatment in PD-MSCs markedly obstructs PD-MSC stemness through AhR signaling. Noteworthy, SR1-mediated MSC application will contribute to new perspectives on MSC-based therapies for air pollution-related bone diseases.

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Experimental exposure of blue mussels ( Mytilus galloprovincialis ) to high levels of benzo[ a ]pyrene and possible implications for human health

Cited by 33 publications

References 52 publications

Acute benzo[a]pyrene exposure induced oxidative stress, neurotoxicity and epigenetic change in blood clam Tegillarca granosa

Acute benzo[a]pyrene exposure induced oxidative stress, neurotoxicity and epigenetic change in blood clam Tegillarca granosa

Accumulation, Depuration, and Biological Effects of Polystyrene Microplastic Spheres and Adsorbed Cadmium and Benzo(a)pyrene on the Mussel Mytilus galloprovincialis

Environmental Benzopyrene Attenuates Stemness of Placenta-Derived Mesenchymal Stem Cells via Aryl Hydrocarbon Receptor

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