Experimental Infection of Calves with an Adenovirus Isolated from Sheep and Related to Bovine Adenovirus Type 2

Belák, Sándór; Pálfi, V.; Szekeres, Thomas; Túry, E.

doi:10.1111/j.1439-0450.1977.tb01023.x

Cited by 11 publications

(6 citation statements)

References 10 publications

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“…Such events in primates, for example, may have contributed to the evolution of AdV now infecting humans (32). Second, mechanisms for AdV host adaption or cross-infection clearly exist, as demonstrated by OAdV-A genotypes, which have maintained the ability to infect both sheep and cattle (3). Additional evidence for expanded host ranges has been reported for several other AdV, including OdAdV (47), BAdV-6 (5), canine AdV (18), and HAdV C viruses (19,21,31,39).…”

Section: Discussionmentioning

confidence: 99%

Detection of Known and Novel Adenoviruses in Cattle Wastes via Broad-Spectrum Primers

Sibley

Goldberg

Pedersen

2011

Appl Environ Microbiol

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The critical assessment of bovine adenoviruses (BAdV) as indicators of environmental fecal contamination requires improved knowledge of their prevalence, shedding dynamics, and genetic diversity. We examined DNA extracted from bovine and other animal waste samples collected in Wisconsin for atadenoviruses and mastadenoviruses using novel, broad-spectrum PCR primer sets. BAdV were detected in 13% of cattle fecal samples, 90% of cattle urine samples, and 100% of cattle manure samples; 44 percent of BAdV-positive samples contained both Atadenovirus and Mastadenovirus DNA. Additionally, BAdV were detected in soil, runoff water from a cattle feedlot, and residential well water. Overall, we detected 8 of 11 prototype BAdV, plus bovine, rabbit, and porcine mastadenoviruses that diverged significantly from previously reported genotypes. The prevalence of BAdV shedding by cattle supports targeting AdV broadly as indicators of the presence of fecal contamination in aqueous environments. Conversely, several factors complicate the use of AdV for fecal source attribution. Animal AdV infecting a given livestock host were not monophyletic, recombination among livestock mastadenoviruses was detected, and the genetic diversity of animal AdV is still underreported. These caveats highlight the need for continuing genetic surveillance for animal AdV and for supporting data when BAdV detection is invoked for fecal source attribution in environmental samples. To our knowledge, this is the first study to report natural BAdV excretion in urine, BAdV detection in groundwater, and recombination in AdV of livestock origin.

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Section: Discussionmentioning

confidence: 99%

Detection of Known and Novel Adenoviruses in Cattle Wastes via Broad-Spectrum Primers

Sibley

Goldberg

Pedersen

2011

Appl Environ Microbiol

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“…In a previous paper (4) we reported on the isolation of a virus (strain Het/3) from nasal discharge of lambs with respiratory symptoms. The strain was related to bovine adenovirus type 2 , and in colostrum-deprived lambs caused respiratory and enteric disease (5,11).…”

Section: Discussionmentioning

confidence: 99%

“…However, in a flock of 4 months old Merino sheep kept under natural conditions and showing signs of respiratory disease an adenovirus (strain Het/3) could be isolated from the nasal discharge of the affected animals (4). Fxperimental infection with this strain led to a respiratory and enteric disease in colostrum-deprived lambs (5,11). The pathogenicity of the isolate is of particular interest, because several adeiiovirus strains have been isolated from sheep so far ( 6 , 7, 8, lo), but their pathogenicity remained unclear.…”

Section: Introductionmentioning

confidence: 99%

Adenovirws Infection in Lambs

Belák¹,

Pálfi²,

Palya³

2010

Zentralblatt Für Veterinärmedizin Reihe B

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Summary A contagious pneumo‐enteritis occurred in suckling and fattening lambs on two large‐scale farms. It started with enteritis and temperature elevation after an incubation period of 7–8 days, which was accompanied by respiratory symptoms 2–3 days later. Diarrhoea lasted for 7 days while respiratory symptoms were observed for another week. Secondary bacterial infection occurred very often, resulting in heavy losses. Pathological and histological examinations revealed the most characteristic changes in the lungs, upper respiratory tract, kidneys and lymph nodes belonging to the respiratory tract and in the intestines. From acutely diseased animals two adenovirus strains, ORT/111 and GY/14 were isolated. Reconvalescent sera contained neutralizing antibodies to the isolates in 1: 16 — 1: 256 titres in contrast to < 1: 2 titres observed with sera obtained at the onset of the disease. In cross neutralization tests strain ORT/111 was closely related to strain Het/3, while strain GY/14 was closely related to ovine adenovirus type 1. Involvement of the isolated strains in the outbreaks is supported by the observation that vaccinated animals resisted challenge infection, whereas control animals showed similar symptoms to those observed in the field cases. Zusammenfassung Adenovirusinfektionen bei Lämmern I. Epidemiologie Es werden Ausbrüche ansteckender Pneumoenteritis bei Saug‐ und Mastlämmern in 2 Betrieben mit intensiver Schafhaltung beschrieben. Nach einer Inkubation von 7–8 Tagen begann die Krankheit mit Fieber und Diarrhöe, 2–3 Tage später kamen respiratorische Symptome hinzu. Die Diarrhöe dauerte im allgemeinen 1 Woche, die akuten respiratorischen Symptome 10–14 Tage. Bakterielle Komplikationen waren häufig und führten zu großen Verlusten. Pathologisch‐anatomisch und histopathologisch ließen sich in den oberen Luftwegen, in der Lunge, den Nieren, sowie in den zu den Luftwegen und zum Darmkanal gehörigen Lymphknoten die charakteristischsten Veränderungen nachweisen. 2 isolierte Adenovirusstämme zeigten serologische Beziehungen zum Serotyp 2 der bovinen Adenoviren, bzw. zum Serotyp 1 der Schafadenoviren. Die Titer der Serumantikörper gegenüber den isolierten Virusstämmen waren bei Beginn der Erkrankungen kleiner als 1: 2, bei Rekonvaleszenten betrugen sie 1: 16— 1: 256. Experimentelle Infektionen führten zu ähnlichen Symptomen wie bei natürlicher Erkrankung. Vakzinierte Tiere blieben nach experimenteller Infektion gesund. Résumé Infections à Adenovirus chez des agneaux I. Epidémiologie On décrit l'apparition de pneumoentérite infectieuse chez des agneaux de lait et d'engrais dans deux exploitations d'élevage intensif de moutons. La maladie a commené après une incubation de 7–8 jours avec fièvre et diarrhée, suivie 2–3 jours plus tard de symptômes respiratoires. La diarrhée dura en général une semaine et les symptómes respiratoires 10–14 jours. Des complications bactériennes furent fréquentes et amenèrent de nombreuses pertes. Des lésions caractéristiques ont été mises en évidence anatomo‐pathologiquement et histo...

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“…The prototype strain No. 19 of subtype A usually is apathogenic but sometimes causes mild respiratory and enteric symptoms in experimentally infected calves and sheep, whereas strain ORT/111 of subtype B is more pathogenic and causes severe pneumoenteritis in both species [7,8].…”

Section: Introductionmentioning

confidence: 99%

First Isolation, Molecular Characterization, and Serological Survey of Bovine Adenovirus Type 2 in Japan

Kubota

Orihara

Kumagai

et al. 2021

Preprint

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Bovine adenovirus type 2 (BAdV-2), a member of the Mastadenovirus genus of the Adenoviridae family, is involved in mild respiratory disease and is rarely isolated from cattle in the world. Here, we isolated BAdV-2 for the first time in Japan, from the feces of cattle with severe respiratory symptoms. The isolate, designated strain KY19-1, was identified morphologically and genetically. Electron microscopic observation of infected cells showed intranuclear, non-enveloped virus particles, approximately 70 nm in diameter and with typical adenoviral morphology. Whole-genome sequencing revealed that the viral genome is 33,175 bp long, with 105 bp inverted terminal repeats and encoding 32 predicted genes. KY19-1 has a similar genomic organization to the prototype BAdV-2 strain No. 19 with overall 99.1% nucleotide identity. Notable differences were found in the E3 region of the genome, which may affect various biological properties, including pathogenicity. The seroepidemiology of BAdV-2 was also investigated, using a virus-neutralization test, and 92.8 % of the Japanese cattle (n=1,325) were seropositive, with the positivity rate increased by age. Further experimental infections with KY19-1 may elucidate the pathogenic properties of BAdV-2 and the importance of this virus as a causal agent of bovine respiratory disease.

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Experimental Infection of Calves with an Adenovirus Isolated from Sheep and Related to Bovine Adenovirus Type 2

Cited by 11 publications

References 10 publications

Detection of Known and Novel Adenoviruses in Cattle Wastes via Broad-Spectrum Primers

Detection of Known and Novel Adenoviruses in Cattle Wastes via Broad-Spectrum Primers

Adenovirws Infection in Lambs

First Isolation, Molecular Characterization, and Serological Survey of Bovine Adenovirus Type 2 in Japan

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