Experimental infection of golden hamsters with Guama virus (Peribunyaviridae, Orthobunyavirus)

Matos, Gabriel Costa; Ferreira, Milene Silveira; Filho, Arnaldo Jorge Martins; Neto, Orlando Pereira Amador; Campos, Valter Miranda; Lima, Maria de Lourdes Gomes; Rodrigues, Jéssica Cecília Pinheiro; Ribeiro, Ana Cláudia da Silva; Freitas, Maria Nazaré Oliveira; Silva, Franko de Arruda e; Chiang, Jannifer Oliveira; Casseb, Lívia Medeiros Neves; Sousa, Jorge Rodrigues de; Quaresma, Juarez Antônio Simões; Martins, Lívia Carício; Vasconcelos, Pedro Fernando da Costa; Carvalho, Valéria Lima

doi:10.1016/j.micpath.2019.103627

Cited by 6 publications

(4 citation statements)

References 10 publications

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“…For the histopathological analysis, tissues of EBIV-infected and mock-infected mice fixed with 10% paraformaldehyde were embedded in paraffin, cut, and stained with hematoxylin-eosin (HE), and examined under light microscopy. For the IHC assay, the antiserum against EBIV-NP recombination protein produced in BALB/c mice was diluted at 1:100, and the histological sections were cut to 4-5 μm for immunohistochemical assay as previously described (Sawatsky et al, 2014;Matos et al, 2019). The stained sections were evaluated for EBIV immunoreactivity by Image-Pro Plus 6.0 software (Media Cybernetics, Inc., Silver Spring, MD, United States), and the accumulated optical density (IOD) and the corresponding brown-yellow positive area were provided with three randomly selected fields of view (200×).…”

Section: Histopathology and Immunohistochemical Assaymentioning

confidence: 99%

Pathogenesis and Immune Response of Ebinur Lake Virus: A Newly Identified Orthobunyavirus That Exhibited Strong Virulence in Mice

et al. 2021

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Orthobunyaviruses are a group of viruses with significant public and veterinary health importance. These viruses are mainly transmitted through mosquito-, midge-, and tick-vectors, and are endemic to various regions of the world. Ebinur Lake virus (EBIV), a newly identified member of Orthobunyavirus, was isolated from Culex mosquitoes in Northwest China. In the present study, we aimed to characterize the pathogenesis and host immune responses of EBIV in BALB/c mice, as an animal model. Herein, we determined that BALB/c mice are highly susceptible to EBIV infection. The infected mice exhibited evident clinical signs including weight loss, mild encephalitis, and death. High mortality of mice was observed even with inoculation of one plaque-forming unit (PFU) of EBIV, and the infected mice succumbed to death within 5–9 days. After EBIV challenge, rapid viremic dissemination was detected in the peripheral tissues and the central nervous system, with prominent histopathologic changes observed in liver, spleen, thymus, and brain. Blood constituents’ analysis of EBIV infected mice exhibited leukopenia, thrombocytopenia, and significantly elevated ALT, LDH-L, and CK. Further, EBIV infection induced obvious cytokines changes in serum, spleen, and brain in mice. Collectively, our data describe the first study that systematically examines the pathogenesis of EBIV and induced immune response in an immunocompetent standard mouse model, expanding our knowledge of this virus, which may pose a threat to One Health.

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Section: Histopathology and Immunohistochemical Assaymentioning

confidence: 99%

Pathogenesis and Immune Response of Ebinur Lake Virus: A Newly Identified Orthobunyavirus That Exhibited Strong Virulence in Mice

et al. 2021

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“…Hepatitis is characterized by perivascular infiltrates as seen in Figure 2 , but it is unclear what cell types are involved in this case. Viruses from multiple families within the order Bunyavirales characteristically produce liver destruction, including many viruses in the family Peribunyaviridae : Caraparu (group C) [ 24 ], Patois [ 25 ], Guama [ 26 ] Oropouche [ 27 ]; Phleboviridae : Rift Valley fever virus [ 28 , 29 , 30 ]; Nairoviridae : Crimean Congo hemorrhagic fever virus [ 29 ] and Hantaviridae : hemorrhagic fever with renal syndrome (hantaviruses) [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Viral Ecology and Natural Infection Dynamics of Kaeng Khoi Virus in Cave-Dwelling Wrinkle-Lipped Free-Tailed Bats (Chaerephon plicatus) in Thailand

Neill

Kading

2021

Diseases

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Kaeng Khoi virus (KKV; Order: Bunyavirales), is an endemic viral infection of the wrinkle-lipped free-tailed bat (Chaerephon plicatus aka Tadarida plicata plicata). Little is known about the ecology and maintenance of KKV within the bat population, nor the infection dynamics and transmission among bats or between bats and other vertebrates. Therefore, KKV was studied in Kaeng Khoi cave, Saraburi province, Thailand, during 1973–1974 with the objectives to (1) characterize the seasonal infection rates of KKV in the context of the bat population ecology, and (2) describe the infection dynamics and viral shedding by naturally- and experimentally-infected bats. To this end, the free-tailed bat population was estimated by a series of timed photographs taken during the evening exodus. The case population of 900,000 adult bats doubled at the time of weaning of the young and returned to its previous level soon thereafter. The newborn bats had neutralizing antibodies to KKV that were likely to be maternal in origin. The KKV antibody prevalence in adult bats was high (69–91%) in March–May and low (29–40%) in August and September. Kaeng Khoi virus was isolated from 75% of dead and 50% of moribund bats, but was not found in nearly 400 apparently healthy bats. Virus was present in saliva, urine and blood of most of the naturally-moribund bats tested. Consistent with observations from naturally-infected bats, experimental infection of bats with KKV revealed significant liver pathology, also suggestive that this is not a benign infection. Kaeng Khoi virus is an endemic, year-round infection maintained by the annual recruitment of a large number of immunologically-naïve juvenile bats. Moreover, it produces an acute infection in the bat, either leading to death by hepatitis, or immunity.

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“…The disease usually lasts for 4–5 days, and patients have acute febrile illness symptoms (fever, dizziness, headache, muscle pain, arthralgia, photophobia) [154, 156]. In experimentally infected laboratory mice, the virus creates lesions in the CNS [148], but in an experimental infection of golden hamsters, the liver was most affected by GMAV [157].…”

Section: Peribunyaviridaementioning

confidence: 99%

The role of sand flies as vectors of viruses other than phleboviruses

Jancarova

Polanska

Volf

et al. 2023

Journal of General Virology

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Sand flies (Diptera: Phlebotominae) are proven vectors of various pathogens of medical and veterinary importance. Although mostly known for their pivotal role in the transmission of parasitic protists of the genus Leishmania that cause leishmaniases, they are also proven or suspected vectors of many arboviruses, some of which threaten human and animal health, causing disorders such as human encephalitis (Chandipura virus) or serious diseases of domestic animals (vesicular stomatitis viruses). We reviewed the literature to summarize the current published information on viruses detected in or isolated from phlebotomine sand flies, excluding the family Phenuiviridae with the genus Phlebovirus, as these have been well investigated and up-to-date reviews are available. Sand fly-borne viruses from four other families (Rhabdoviridae, Flaviviridae, Reoviridae and Peribunyaviridae) and one unclassified group (Negevirus) are reviewed for the first time regarding their distribution in nature, host and vector specificity, and potential natural transmission cycles.

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Experimental infection of golden hamsters with Guama virus (Peribunyaviridae, Orthobunyavirus)

Cited by 6 publications

References 10 publications

Pathogenesis and Immune Response of Ebinur Lake Virus: A Newly Identified Orthobunyavirus That Exhibited Strong Virulence in Mice

Pathogenesis and Immune Response of Ebinur Lake Virus: A Newly Identified Orthobunyavirus That Exhibited Strong Virulence in Mice

Viral Ecology and Natural Infection Dynamics of Kaeng Khoi Virus in Cave-Dwelling Wrinkle-Lipped Free-Tailed Bats (Chaerephon plicatus) in Thailand

The role of sand flies as vectors of viruses other than phleboviruses

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