Experimental infection of macaques with HIV-2ben, a novel HIV-2 isolate

Stahl–Hennig∥, Christiane; Herchenröder, Ottmar; Nick, Sigrid; Evers, Maria; Stille-Siegener, Michael; Jentsch, Klaus Dieter; Kirchhoff, Frank; Tolle, Tanja K.; Gatesman, T.J.; Lüke, Wolfgang; Hunsmann, Gerhard

doi:10.1097/00002030-199007000-00001

Cited by 64 publications

(27 citation statements)

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“…The cell free supematants were added to C8166 indicator cells (Salahuddin et al, 1983). Peripheral blood lymphocytes (PBLs) were prepared as described (Stahl-Hennig et al, 1990) Cells were surface labelled by incubating 2 X 10 5 cells for 30 min at 4 0 C in 100 pI phosphate buffered saline (PBS), pH 7.2, containing 0.2% bovine serum albumin (BSA), 0,02% sodium azide and the respective antibodies. The cells were then washed in the same buffer.…”

Section: Isolation and Detection Ofvirusmentioning

confidence: 99%

Early phenotypic and functional alterations in lymphocytes from simian immunodeficiency virus infected macaques

Kneitz

Kerkau

Müller

et al. 1993

Veterinary Immunology and Immunopathology

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Kneitz, c., Kerkau, T., Müller, J., Coulibaly, C., Stahl-Hennig, c., Hunsmann, G., Hünig, T. and Schimpl, A., 1993. Early phenotypic and functional alterations in Iymphocytes from simian immunodeficiency virus infected macaques. Veto Immunol. Immunopathol., Phenotypic and functional changes in Iymphocytes from rhesus monkeys (Macaca mulatta) were investigated during the first 6 months after infection with SIVmac 32H. Animals preimmunized with keyhole !impet hemocyanin (KLH) were sacrificed 1. 3, 6, 12, and 24 weeks post infection. Subset composition and function oflymphocytes from blood, spleen, Iymph node and thymus were analysed. In addition to a rapid decline in CD4/CD8 ratios, a massive reduction in CD29+CD4+ cells was seen in the periphery. Although depletion of this subset was observed throughout the course of this experiment, the loss of proliferative T cell responses was most pronounced very early after infection and partially recovered after Month 3. Polyclonal cytotoxic responses were only slightly affected. In the thymus, a gradual, but moderate loss of CD4 + CD8 + immature thymocytes, and a relative increase in both CD4 + and CD8 + mature subsets was observed. Infectious virus was readily reeovered from homogenates oflymph node and spleen, but not of thymus tissue. Interestingly, however, virus was deteeted in thymocytes from a11 infected animals by cocultivation with a simian immunodeficiency virus (SIV) susceptible celliine. ABBREVIATIONSAb, antibody; BSA, bovine serum albumin; BSS, balaneed salt solution; DN, double negative; DP, double positive; FCS, fetal calf serum; KLH, keyhole Iimpet hemocyanin; mAb, monoc1onal antibody, PBL, peripheral blood Iymphoeyte; PBS, phosphate buffered saline; SIV, simian immunodeficieney virus; SP, single positive.

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Section: Isolation and Detection Ofvirusmentioning

confidence: 99%

Early phenotypic and functional alterations in lymphocytes from simian immunodeficiency virus infected macaques

Kneitz

Kerkau

Müller

et al. 1993

Veterinary Immunology and Immunopathology

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“…Rhesus monkeys were housed at the German Primate Center in Gottingen. Handling of the monkeys and collection of specimens were performed according to institutional guidelines as described (32). Two rhesus monkeys of Indian origin (seronegative for SIV, D-type retroviruses, and simian T-cell lymphotropic virus type 1) were infected intravenously with 3000 TCID50 of RT-SHIV.…”

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confidence: 99%

Animal model for the therapy of acquired immunodeficiency syndrome with reverse transcriptase inhibitors.

Überla

Stahl–Hennig∥

Böttiger

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

122

116

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The reverse transcriptase (RT) of the human immunodeficiency virus type 1 (HIV-1) is the major target for antiretroviral therapy of the acquired immunodeficiency syndrome (AIDS). While some inhibitors exhibit activity against most retroviral RTs, others are specific for the HIV-1 enzyme.To develop an animal model for the therapy of the HIV-1 infection with RT inhibitors, the RT of the simian immunodeficiency virus (SIV) was replaced by the RT of HIV-1.

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“…The rhesus macaques (Macaca mulatta) used for this study were housed at the German Primate Center in Go$ ttingen, Germany. Handling of the monkeys and collection of specimens were performed according to institutional guidelines as described previously (Stahl-Hennig et al, 1990). The four rhesus monkeys, Mm 7755, 7756, 7761 and 7763, were infected intravenously with 300 TCID &!…”

Section: Methodsmentioning

confidence: 99%

Specificity of helper T-cells generated from macaques infected with attenuated simian immunodeficiency virus.

Dittmer

Feldmann

Sauermann

et al. 1998

Journal of General Virology

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Deletion of the simian immunodeficiency virus (SIV)nef gene leads to an attenuated virus phenotype in vivo. We have previously shown that these viruses induce a potent cellular immune response in macaques. To extend these studies, we established virus-specific short-term T-cell lines from four rhesus macaques infected with a nef deletion mutant of SIV. These T-cell lines proliferated upon restimulation with whole SIV or SIV gp140 antigen in vitro. The proliferating cells were characterized as CD4 M helper T-cells (TH) and their antigen recognition was MHC class II DR-restricted. After antigenic stimulation, they transcribed mRNA for various TH1-and TH2-like cytokines. Using these SIV-specific cell

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Experimental infection of macaques with HIV-2ben, a novel HIV-2 isolate

Cited by 64 publications

References 0 publications

Early phenotypic and functional alterations in lymphocytes from simian immunodeficiency virus infected macaques

Early phenotypic and functional alterations in lymphocytes from simian immunodeficiency virus infected macaques

Animal model for the therapy of acquired immunodeficiency syndrome with reverse transcriptase inhibitors.

Specificity of helper T-cells generated from macaques infected with attenuated simian immunodeficiency virus.

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