Experimental infection of Mouflon-domestic sheep hybrids with caprine arthritis-encephalitis virus

Guiguen, François; Mselli-Lakhal, Laïla; Durand, J.-P.; Du, Jian; Favier, Colette; Fornazero, Claudie; Grézel, Delphine; Balleydier, Sabine; Hausmann, Elda H.S.; Chebloune, Yahia

doi:10.2460/ajvr.2000.61.456

Cited by 37 publications

(26 citation statements)

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“…To date, no such viruses have been isolated from sheep and goats, which suggests that "exotic" SRLVs are not a cause of concern for domestic small ruminants. Moufflon and ibex are the closest relatives of sheep and goats respectively, which may explain the existence of SRLVs in these species [58]. There is no evidence that, under natural conditions, SRLVs can infect species such as cattle that are not closely related to sheep and goats.…”

Section: The Risk Of the Transmission Of Srlvs To Other Speciesmentioning

confidence: 99%

Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication schemes

et al. 2004

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-Small ruminant lentiviruses (SRLV = maedi-visna in sheep and caprine arthritis encephalitis in goats) are distributed throughout most countries of the world, particularly Europe. Laboratories from 16 European countries established collaborations within the framework of a COST (CO-operation in the field of Scientific and Technical Research) action sponsored by the European Union in order to (i) better organize their research programmes on SRLVs and (ii) to coordinate efforts to combat these two diseases. After five years, a consensus conference -the first one in the veterinary medicine field -concluded the work of this network of laboratories by reviewing the present position and discussing three important questions in the field of SRLVs: routes of transmission, consequences of infection and potential role of eradication programmes at either a European or local level, according to the situation in each country or region. This paper brings together existing information regarding these questions and identifies areas for future research.Maedi-visna / caprine arthritis-encephalitis virus / lentivirus / small ruminants / European consensus conference

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Section: The Risk Of the Transmission Of Srlvs To Other Speciesmentioning

confidence: 99%

Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication schemes

et al. 2004

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“…The receptor(s) used by CAEV to enter the cells is still unknown. In our recent studies of CAEV species tropism, we found that this virus is capable of infecting cells from a very large variety of mammalian species including moufflon, cattle and pig ( [19], unpublished results). This suggests either that the receptor is widely expressed, or that CAEV can use various receptors to infect target cells.…”

Section: Discussionmentioning

confidence: 92%

Immortalized goat milk epithelial cell lines replicate CAEV at high level

et al. 2001

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-Primary milk epithelial cells were isolated from CAEV-uninfected goats and three cell lines designated TIGMEC-1, TIGMEC-2 and TIGMEC-3 were established. The three cell lines retained their morphological characteristics of epithelial cells and expressed specific epithelial cytokeratin marker as well as the immortalizing SV40 large T antigen. The kinetics of growth of TIGMEC1, TIG-MEC2 and TIGMEC3 cell lines showed a doubling time of 24-48 hours while the parental cell lines became senescent after the passage 6 in cell culture. Like the parental primary cells, the three cell lines were found to be highly sensitive to CAEV-pBSCA, an infectious molecular clone of CAEV-CO strain, and to a French isolate CAEV-3112. TIGMEC cell lines infected with CAEV-pBSCA became chronically infected producing high virus titers in absence of cytopathic effects. These cell lines may be useful for study of the possible physiological alterations in mammary epithelial cells infected with small ruminant lentiviruses and their consequences on milk quality. On an other hand, these cell lines can be used to study their role in virus transmission and pathogenesis. CAEV / replication / epithelial cell lineRésumé -Des cellules épithéliales du lait immortalisées répliquent le CAEV avec une grande efficacité. Des cellules épithéliales primaires isolées à partir de chèvres non infectées par le CAEV ont été immortalisées pour dériver 3 lignées appelées TIGMEC-1, TIGMEC-2 et TIGMEC-3. Les cellules des 3 lignées ont conservé leurs caractéristiques morphologiques de cellules épithéliales et expriment la cytokératine, un marqueur spécifique des cellules épithéliales et l'antigène immortalisant T du virus SV40. La courbe de croissance des lignées TIGMEC-1, TIGMEC-2 et TIGMEC-3 montre une multiplication des cellules toutes les 24 à 48 heures alors que les cellules parentales ne Vet. Res. 32 (2001) 429-440 429

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“…Estudos filogenéticos indicam a existência de transmissão entre caprinos e ovinos (Zanoni et al 1998, Castro et al 1999a. Já foi demonstrada a transmissão experimental para outros bovídeos (Guiguen et al 2000). A transmissão venérea ainda não foi confirmada, apesar da detecção de RNA viral no sêmen de caprinos experimentalmente infectados (Travassos et al 1998), de caprinos naturalmente infectados (Travassos et al 1999), de carneiros naturalmente infectados (Chastang et al, comunicação pessoal) e de ovino experimentalmente infectado por SRLV e Brucela ovis (Concha-Bermejillo et al 1996).…”

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Lentivírus de pequenos ruminantes (CAEV e Maedi-Visna): revisão e perspectivas

2001

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lentivírus de pequenos ruminantes (SRLV), cujos protótipos são os vírus da Artrite-Encefalite Caprina (CAEV) e Maedi-Visna, são patógenos amplamente distribuidos, os quais causam doenças degenerativas progressivas lentas em caprinos e ovinos, determinando importantes perdas econô-micas. Estes vírus causam infecções persistentes com perío-do de incubação longo e causam inflamatórias e degenerativas. As lesões são induzidas em tecidos específicos do hospedeiro como articulações, pulmões, CNS e glandulas mamárias devido à replicação viral em células da linhagem monocítico-fagocitária que são as principais células-alvo. A infecção ocorre principalmente durante os primeiros meses de vida, através da ingestão de vírus no leite ou colostro de cabras ou ovelhas infectadas. A indução da resposta imunológica é variável e não protege contra a infecção. O diagnós-tico é baseado primariamente na detecção de anticorpos para SRLV, geralmente por imunodifusão em gel de agar (AGID) e enzyme linked immunosorbent assay (ELISA). O diagnóstico e separação ou descarte dos animais soropositivos associado ao uso de certas práticas de manejo, especialmente das crias, são os principais meios implementados para prevenir a dis- Small ruminant lentiviruses (SRLV), whose prototypes are Caprine Arthritis-Encephalitis virus (CAEV) and Maedi-Visna virus, are the causative agents of slow progressive degenerative diseases of goats and sheep (infected animals), responsible for significant economic losses. These viruses cause persistent infections with long periods of incubation and induce inflammatory and degenerative lesions. The lesions are induced in target organs of the host such as joints, CNS, lungs and mammary glands due to viral replication in cells of the monocyte/ macrophage lineage which is the main target cell. Infections occur particularly in the young and are acquired through ingestion of virus in milk or colostrum from infected does or ewes. The induction of immune response is variable and does not protect against the infection. Diagnosis is primarily based on the presence of SRLV antibodies usually detected by agar gel immunodiffusion (AGID) or enzyme linked immunosorbent assays (ELISA). As no vaccine is available, most often employed schemes to prevent spread of SRLV are based on segregation or/and culling of positive animals associated with management practices, especially the offspring. The strategies of SRLV for dealing with the immune system make difficult to accomplish diagnosis of infection, control or prevention of the viral spread. This review shows aspects of SRLV based on their phylogenetic studies of fields isolates, clinical, and immunopathological features.INDEX TERMS: Small ruminant lentiviruses, caprine arthritis-encephalitis virus, maedi-visna, retroviruses, phylogenetic analysis, epidemiology, goat, sheep.

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Experimental infection of Mouflon-domestic sheep hybrids with caprine arthritis-encephalitis virus

Abstract: Results indicate that Mouflon-domestic sheep hybrids are susceptible to infection with isolates of CAEV that cause infection in domestic small ruminants.

Cited by 37 publications

References 37 publications

Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication schemes

Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication schemes

Immortalized goat milk epithelial cell lines replicate CAEV at high level

Lentivírus de pequenos ruminantes (CAEV e Maedi-Visna): revisão e perspectivas

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