Experimental Infection of Ponies with <i>Borrelia burgdorferi</i> by Exposure to Ixodid Ticks

Chang, Yung-Fu; Novosol, V.; McDonough, Sean P.; Chang, Chia‐Ming; Jacobson, Richard H.; Divers, Thomas J.; Quimby, Fred W.; Shin, Seung Uon; Lein, Donald H.

doi:10.1354/vp.37-1-68

Cited by 81 publications

(89 citation statements)

References 31 publications

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“…The sequence of the forward primer was 5Ј-GGAATTCATGTTAAAGTCACTGCT-3Ј, and that of the reverse primer was 5Ј-CCGCTCGAGGTTTTAATAGAGGC-3Ј. Amplification conditions were as previously described (5). PCR products were purified by using a gel purification kit (Qiagen) and digested with BamHI and HindIII to detect restriction polymorphisms.…”

Section: Methodsmentioning

confidence: 99%

Cloning and Molecular Characterization of an Immunogenic LigA Protein of Leptospira interrogans

et al. 2002

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A clone expressing a novel immunoreactive leptospiral immunoglobulin-like protein A of 130 kDa (LigA) from Leptospira interrogans serovar pomona type kennewicki was isolated by screening a genomic DNA library with serum from a mare that had recently aborted due to leptospiral infection. LigA is encoded by an open reading frame of 3,675 bp, and the deduced amino acid sequence consists of a series of 90-amino-acid tandem repeats. A search of the NCBI database found that homology of the LigA repeat region was limited to an immunoglobulin-like domain of the bacterial intimin binding protein of Escherichia coli, the cell adhesion domain of Clostridium acetobutylicum, and the invasin of Yersinia pestis. Secondary structure prediction analysis indicates that LigA consists mostly of beta sheets with a few alpha-helical regions. No LigA was detectable by immunoblot analysis of lysates of the leptospires grown in vitro at 30°C or when cultures were shifted to 37°C. Strikingly, immunohistochemistry on kidney from leptospira-infected hamsters demonstrated LigA expression. These findings suggest that LigA is specifically induced only in vivo. Sera from horses, which aborted as a result of natural Leptospira infection, strongly recognize LigA. LigA is the first leptospiral protein described to have 12 tandem repeats and is also the first to be expressed only during infection.

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Section: Methodsmentioning

confidence: 99%

Cloning and Molecular Characterization of an Immunogenic LigA Protein of Leptospira interrogans

et al. 2002

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“…Sera from horses vaccinated with commercial leptospira pentavalent bacterin were obtained from horses in a riding stable in north Georgia. Sera from horses infected with Borrelia burgdorferi (10) and Ehrlichia sp. (11) were kindly provided by Yung-Fu Chang (Cornell University, Ithaca, NY).…”

Section: Methodsmentioning

confidence: 99%

Host-Inducible Immunogenic Sphingomyelinase-Like Protein, Lk73.5, ofLeptospira interrogans

et al. 2004

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“…Kinetics-ELISA (KELA) and Western blot analysis were performed as previously described (4,5,7). KELA for measuring the relative quantity of serum antibody to B. burgdorferi was performed as described previously.…”

Section: Methodsmentioning

confidence: 99%

Human Granulocytic Ehrlichiosis Agent Infection in a Pony Vaccinated with aBorrelia burgdorferiRecombinant OspA Vaccine and Challenged by Exposure to Naturally Infected Ticks

Chang¹,

McDonough²,

Chang³

et al. 2000

Clin Diagn Lab Immunol

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A pony was vaccinated with recombinant OspA vaccine (rOspA) and then exposed 3 months later to Borrelia burgdorferi-infected ticks (Ixodes scapularis) collected in Westchester County, N.Y. At 2 weeks after tick exposure, the pony developed a high fever (105°F). Buffy coat smears showed that 20% of neutrophils contained ehrlichial inclusion bodies (morulae). Flunixin Meglumine (1 g daily) was given for 2 days, and the body temperature returned to normal. PCR for ehrlichial DNA was performed on blood samples for 10 consecutive days beginning when the pony was first febrile. This pony was monitored for another 3.5 months but developed no further clinical signs. The 44-kDa immunodominant human granulocytic ehrlichiosis antigen gene was amplified by PCR and cloned into a pCR2.1 vector. DNA sequence analysis of this gene showed it was only 8 bp different (99% identity) from the results reported by others (J.W. Ijdo et al., Infect. Immun. 66:3264-3269, 1998). Western blot analysis, growth inhibition assays, and repeated attempts to isolate B. burgdorferi all demonstrated the pony was protected against B. burgdorferi infection. These results highlight the potential for ticks to harbor and transmit several pathogens simultaneously, which further complicates the diagnosis and vaccination of these emerging tick-borne diseases.

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Experimental Infection of Ponies with Borrelia burgdorferi by Exposure to Ixodid Ticks

Cited by 81 publications

References 31 publications

Cloning and Molecular Characterization of an Immunogenic LigA Protein of Leptospira interrogans

Cloning and Molecular Characterization of an Immunogenic LigA Protein of Leptospira interrogans

Host-Inducible Immunogenic Sphingomyelinase-Like Protein, Lk73.5, ofLeptospira interrogans

Human Granulocytic Ehrlichiosis Agent Infection in a Pony Vaccinated with aBorrelia burgdorferiRecombinant OspA Vaccine and Challenged by Exposure to Naturally Infected Ticks

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