Using 18-beta-glycyrrhetinic acid, a well-known component of licorice (Glycyrrhiza glabra Lin.), the current study offers a unique rapid ecological & simple approach of biologically synthesizing of silver nanoparticles (AgNPs). To synthesise stable silver nanoparticles via a biological reduction technique, a methanolic stock solution of 18-β-glycyrrhetinic acid was produced and used as capping and reducing agent. The approach was methodically optimized using response surface analysis (RSA) based Box-Behnken design (BBD), taking into account influence of parameters like as silver nitrate (AgNO3) concentration, incubation time and temperature on response. RSA was utilized to determine the association among factors and the responses by mathematical modelling with a quadratic polynomial model. AgNO3 (1mM), 55°C, and 5 hours incubation were optimal. 18-β-glycyrrhetinic acid methanolic stock solution can convert silver ions (Ag+) into silver nanoparticles (AgNPs) in 5 hours at 55°C. Biosynthesized and optimized AgNPs have an SPR absorption peak at 419 nm in their UV spectra. 18-β-glycyrrhetinic acid reduced and capped silver ions according to FTIR spectroscopy. XRD showed AgNPs’ crystallinity. SEM revealed spherical elemental silver with particle size of 100 nm. Average particle size, PDI & Zeta were 83.36 nm, 0.462 and -35.4mV, respectively, at 100% intensity. Silver nanoparticles (GAAgNPs) are stable. DPPH experiment showed substantial antioxidant activity in GAAgNPs compared to ascorbic acid. At 10 μg/mL, AgNPs showed utmost region of inhibition of 15 and 14 mm against Staphylococcus aureus and Pseudomonas aeruginosa, respectively. Finally, the synthesized AgNPs and their quality components have strong antioxidant and antibacterial activity, indicating that this research can be used to formulate useful biomedical goods.