In this work, biodiesel obtained from the transesterfication of Jatropha seeds and Fish wastes is used as an alternative fuel to diesel in stationary single cylinder diesel engines. The biodiesel obtained has good ignition ability due to its relatively high cetane number compared to that of conventional diesel fuel. The performance, combustion and emission tests using Jatropha Oil Methyl Ester (JOME), Fish Oil Methyl Ester (FOME) and their blends (20% JOME and 20% FOME) with diesel were carried out at constant speed and variable loads condition. The results showed that both blends could be used as fuels for diesel engine without any major modification on the engine. Carbon monoxide (CO), UBHC and smoke emissions were observed to be lesser at all loads for both the blends compared to diesel fuel, while NOx emission was slightly higher. JOME blend was found to be better than FOME blend.