The effect of different homogenization parameters on the microstructure and element distribution of Cr8Mo2SiV cold-work die steel ingot is investigated. Based on the Thermo-Calc software and laser confocal microscopy, the optimal heat treatment temperature range from 1160 to 1230 C is obtained for experimental steel. In order to improve dendritic segregation of as-cast microstructure consisting of a large number of network eutectic carbides enriched in alloying elements, the homogenization treatment is carried out at the above determined temperatures for 0.5, 1, and 3 h, respectively. Then, according to the segregation ratio of Cr element, the homogenization at 1180 C for 3 h successfully eliminates the microsegregation, which is in agreement with the calculation of the diffusion kinetic model of Cr element.