Experimental iron deficiency in Syrian hamsters (<i>Mesocricetus auratus</i>)

Ranasinghe, A. W.; Johnson, Newell W.; Rountree, Robert

doi:10.1258/002367783781070786

Cited by 6 publications

(5 citation statements)

References 7 publications

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“…Anaemic mice were lethargic and weighed much less than age-matched controls although they appeared to eat normally. This finding was in agreement with that reported by Ranasinghe et al (1983) who fed a similar diet to Syrian hamsters. This failure to gain weight has been attributed to an iron deficiency-induced anorexia (Canale & Lanzkowsky, 1970).…”

Section: Chronic Anaemiasupporting

confidence: 94%

“…In addition to these methods, a number of studies have been employed using dietary manipulations to produce anaemia (Choi & Simone, 1973;Webster, 1981;Ranasinghe et al, 1983;Walker et al, 1987). Since chronic blood loss with neoplasia ultimately leads to an iron-deficiency anaemia (Lynch & Jackson, 1983), the manipulation of the dietary iron content to produce anaemia was considered to be a relevant model and worthy of investigation.…”

Section: Models Of Anaemiamentioning

confidence: 99%

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Chronic anaemia, hyperbaric oxygen and tumour radiosensitivity

McCormack¹,

Nias²,

Smith³

1990

The British Journal of Radiology

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Anaemia is an important factor in the response of some human tumours to radiotherapy. The outcome is also influenced by whether the treatment is given in air or high pressure oxygen (HPO). The present study examined the relationship between anaemia and tumour response to radiation given in air or HPO in C3H mice transplanted with a mammary adenocarcinoma using a growth delay assay to assess the radiation response. Chronic anaemia was induced by the use of a low iron diet and was characterized by a significant reduction in host haematocrit and whole blood viscosity. In addition, anaemia was associated with a right shift in the oxyhaemoglobin dissociation curve and an increase in the volume doubling time of the tumour; but there was no change in the concentration of 2,3-diphosphoglycerate in the red cells. Radiation studies with these anaemic mice demonstrated that the tumour radiosensitivity was decreased when treatment was given in air. HPO was successful in overcoming the increased radioresistance associated with anaemia. This result suggested that tumours grown in anaemic mice have a higher hypoxic fraction than those grown in control mice. Changes in host physiology with chronic anaemia may contribute to the benefit seen with HPO but such alterations per se may be inadequate to maintain tumour oxygenation when treatment is given in air.

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Section: Chronic Anaemiasupporting

confidence: 94%

Section: Models Of Anaemiamentioning

confidence: 99%

Chronic anaemia, hyperbaric oxygen and tumour radiosensitivity

McCormack¹,

Nias²,

Smith³

1990

The British Journal of Radiology

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“…Rats developed anemia showing decreased body weight and most blood profile parameters such as RBCs count, HB, HCT, MCV, serum iron, TIBC, and TS. Previously, similar findings were observed in hamster and rat models [29,30]. When iron deficiency was corrected with raisin administration, body weight gain was observed.…”

Section: Discussionsupporting

confidence: 83%

Black Raisins Improved Experimentally Induced Iron Deficiency Anemia. Biochemical and Histological Evidence

Zaid

Abduljawad

Aljadani

et al. 2021

JPRI

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Iron deficiency anemia is a challenging clinical problem with a profound impact on general health. Administration of iron-containing preparations were reported to be associated with many side effects. The current study aimed to evaluate black raisins’ therapeutic role against experimentally induced iron deficiency anemia in rats. Forty female rats were divided into 4 groups (n=10); control, anemia, anemia + iron, and anemia + raisins. Anemia was induced by bleeding and an iron-deficient diet for 4 weeks. Anemic rats were treated with ferric sulfate (200 mg/kg) or raisin extract (375 mg/kg) daily for 12 weeks. Complete blood count (CBC), blood films, body weight, splenic weight, and index were assessed. The spleens were processed and stained by hematoxylin and eosin (H & E) and immunohistochemically stained for CD3+ and CD68. Raisins contain a considerable amount of iron, vitamin B, phenolics, and flavonoid antioxidants. In raisins treated rats, CBC parameters displayed a significant increase compared to the anemic rats. Most of the RBCs in blood films showed normal shape, size, and central pallor. The spleen of raisins and iron-treated rats showed a marked increase in the area of white pulp. Their spleens also showed a significant increase in the CD3+ PALS area compared to anemic rats. Iron and raisins significantly decreased splenic CD68 macrophages. Consumption of black raisins could be considered an excellent natural source for flavonoids and iron to be used as an adjuvant supplement to iron for anemic patients with increased splenic T lymphocytes.

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“…They were housed in plastic cages in groups of five or six per cage. Iron deficiency was induced by feeding the animals on a low iron, powdered diet and withdrawing 0.5 ml of blood from the retro-orbital sinus (Pansky et al, 1961) each week over a period of 9 weeks (Ranasinghe, Johnson & Rountree, 1983a). Control animals were fed the same powdered diet supplemented with 240 mg iron (in the form of powdered iron sulphate) per kilogram of food.…”

Section: Animal Modelmentioning

confidence: 99%

“…All animals received distilled water ad libitum and were maintained in constant 12-hr light/dark cycles with illumination from 0600 to 1800 hours. After 9 weeks, when anaemia was established in the animals fed the low iron diet, all animals were exsanguinated via the retro-orbital sinus for haematological analysis, as described by Ranasinghe et al (1983a).…”

Section: Animal Modelmentioning

confidence: 99%

Iron deficiency depresses cell proliferation in hamster cheek pouch epithelium

1987

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Abstract. Iron deficiency anaemia was induced in hamsters by feeding a low iron diet coupled with weekly bleeding. To assess cell proliferation, the stathmokinetic agent vinblastine sulphate was administered and cell birth rates were calculated from cumulative mitotic indices. The rate was significantly reduced in epithelium from iron‐deficient animals. The uptake of tritiated thymidine ([3H]TdR) was also significantly reduced in these animals. Results of both stathmokinetic and labelling experiments indicate that cell production in the cheek pouch epithelium of iron‐deficient animals is impaired.

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Experimental iron deficiency in Syrian hamsters (Mesocricetus auratus)

Cited by 6 publications

References 7 publications

Chronic anaemia, hyperbaric oxygen and tumour radiosensitivity

Chronic anaemia, hyperbaric oxygen and tumour radiosensitivity

Black Raisins Improved Experimentally Induced Iron Deficiency Anemia. Biochemical and Histological Evidence

Iron deficiency depresses cell proliferation in hamster cheek pouch epithelium

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