Experimental measurement and simulation of mechanical strength and biological behavior of porous bony scaffold coated with alginate-hydroxyapatite for femoral applications

Li, Xiang; Saber‐Samandari, Saeed; Beni, Mohsen Heydari; Morovvati, M.R.; Angili, Sajad Niazi; Toghraie, Davood; Khandan, Amirsalar; Khan, Afrasyab

doi:10.1016/j.compscitech.2021.108973

Cited by 21 publications

(10 citation statements)

References 55 publications

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“…Additionally, Alg-hydroxyapatite-coated porous bone scaffolds had improved cell activity, decreased porosity, and higher mechanical strength, making them better suited for use as bone scaffolds. 21 Due to their unique catalytic activity, cerium oxide nanoparticles (nCeO 2 ) have recently gained increasing interest in biomedical applications. By observing alterations in the local tissues and major organs following subcutaneous implantation of nCeO 2 for 28 days in rats, the safety of nCeO 2 was investigated.…”

Section: Introductionmentioning

confidence: 99%

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Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering

Li¹,

Li²,

Song³

et al. 2022

IJN

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Background: Clinicians frequently face difficulties when trying to fix bone abnormalities. Gelatin-Alginate (GA) is frequently employed as a carrier because it is non-toxic, biodegradable, and has a three-dimensional network structure. Meanwhile, cerium oxide nanoparticles (nCeO 2 ) demonstrated high antioxidant enzyme simulation activity. Therefore, in order to develop a porous hydrogel scaffold for the application of bone tissue engineering, an appropriate-type GA-nCeO 2 hydrogel scaffold was developed and evaluated. Methods: GA-nCeO 2 hydrogel scaffold was prepared by the lyophilized method and characterized. The surface morphology and cell adhesion of the scaffold were observed by the scanning electron microscope. CCK8 and live-dead staining methods were used to evaluate its biological safety and cell proliferation. Then the osteogenic differentiation in early and late stages was discussed. The expression of osteogenic genes was also detected by RT-PCR. Finally, a bone defect model was made in SD rats, and bone formation in vivo was detected. Results:The results showed that GA-nCeO 2 hydrogel scaffold exhibited a typical three-dimensional porous structure with a mean pore ratio of 70.61 ± 1.94%. The GA-nCeO 2 hydrogel was successfully endowed with simulated enzyme activity including superoxide dismutase (SOD) and catalase (CAT) after the addition of nCeO 2 . Osteoblasts demonstrated superior cell proliferation and adhesion on composite scaffolds, and both mineralization test and gene expression demonstrated the strong osteogenic potential of GA-nCeO 2 hydrogel. The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats further supported the scaffold's favorable biocompatibility and bone-promoting capacity. Conclusion: Due to its favorable safety, degradability, and bone formation property, GA-nCeO 2 hydrogel was anticipated to be used as a potential bone defect healing material.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering

Li¹,

Li²,

Song³

et al. 2022

IJN

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“…The generated models possess decent 3D structural integrity with the same particle size distributions as actual statistics (Figure S8c−f). Dirichlet boundary conditions 29 are used to calculate the von mises stress contour under 3% tensile strain along the x-axis.…”

Section: Mechanical Behaviorsmentioning

confidence: 99%

Co-Optimizing Insulative and Mechanical Properties of Quartz Fabric Reinforced Phenolic Composites by a Compromising Porous Structure for Thermal Insulation

Qian

Cai

Wang

et al. 2023

Ind. Eng. Chem. Res.

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Introducing porosity into structures is recognized as a practical strategy to achieve lightweightness and insulation for ablatives, but reduced matrix density leads to the weakening of mechanical and anti-ablation performances. Herein, a trade-off design of the porous structure is developed for integrating high-strength, insulation, and anti-ablation abilities into mid-density nanoporous phenolic composites (NPC). Benefiting from a narrow nanopore size (20–62 nm) of the matrix, thermal conductivity of NPC can be effectively limited within 0.079–0.115 W/(m·K), while showing a mid-density of 0.89–1.04 g/cm3. Meanwhile, NPC shows a considerable axial tensile strength of 130.2–177.8 MPa and out-of-plane compressive strength of ∼300 MPa due to the compromising porosity (34–62%) and reinforcing of the 3D needle-punched quartz fiber preform. Besides, NPC exhibits excellent oxidation resistance in static radiation heating (1000 °C) and outstanding insulation and ablation resistance under an oxy-acetylene heat flux of 1.76 and 4.18 MW/m2 with the linear ablation rates of ∼0.102 and ∼0.185 mm/s, respectively. The results will further promote the development and application of phenolic ablatives in extreme re-entry environments.

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“…Several works have been done with calcium phosphate, glass, magnesium oxide, graphite and wollastonite-containing glass-ceramics for developing polymer composite for clinical applications such as bone implant and dentistry (Mohammadi Zerankeshi et al , 2022; Rezwan et al , 2006; Siqueira et al , 2017; Wang, Meng, et al , 2022; Wang, Gu, et al , 2022). The recent advancements in PLA composite with bioactive ceramics such as hydroxyapatite (HA) (Gendviliene et al , 2020; Li et al , 2021; Senatov et al , 2022; Wang et al , 2021; Wu et al , 2020), beta-tricalcium phosphate (Backes et al , 2021; Esslinger et al , 2020; Redondo, 2021; Seidenstuecker et al , 2021), calcium carbonate (Donate et al , 2020), akermanite (Arastouei et al , 2021), bio-glass (Alksne et al , 2020; Palivela et al , 2022) through FDM, has resulted in desirable osteogenicity and mechanical strength in comparison to neat polymer. The bioactive ceramics have a non-load bearing application due to their brittle nature.…”

Section: Introductionmentioning

confidence: 99%

Investigations on effect of pore architectures of additively manufactured novel hydroxyapatite coated PLA/Al₂O₃ composite scaffold for bone tissue engineering

Choudhary

Ghosh²,

Sharma

et al. 2023

RPJ

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Purpose The purpose of this paper is to fabricate the scaffolds with different pore architectures using additive manufacturing and analyze its mechanical and biological properties for bone tissue engineering applications. Design/methodology/approach The polylactic acid (PLA)/composite filament were fabricated through single screw extrusion and scaffolds were printed with four different pore architectures, i.e. circle, square, triangle and parallelogram with fused deposition modelling. Afterwards, scaffolds were coated with hydroxyapatite (HA) using dip coating technique. Various physical and thermo-mechanical tests have been conducted to confirm the feasibility. Furthermore, the biological tests were conducted with MG63 fibroblast cell lines to investigate the biocompatibility of the developed scaffolds. Findings The scaffolds were successfully printed with different pore architectures. The pore size of the scaffolds was found to be nearly 1,500 µm, and porosity varied between 53% and 63%. The fabricated circular pore architecture resulted in highest average compression strength of 13.7 MPa and modulus of 525 MPa. The characterizations showed the fidelity of the work. After seven days of cell culture, it was observed that the developed composites were non-toxic and supported cellular activities. The coating of HA made the scaffolds bioactive, showing higher wettability, degradation and high cellular responses. Originality/value The research attempts highlight the development of novel biodegradable and biocompatible polymer (PLA)/bioactive ceramic (Al2O3) composite for additive manufacturing with application in the tissue engineering field.

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Experimental measurement and simulation of mechanical strength and biological behavior of porous bony scaffold coated with alginate-hydroxyapatite for femoral applications

Cited by 21 publications

References 55 publications

Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering

Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering

Co-Optimizing Insulative and Mechanical Properties of Quartz Fabric Reinforced Phenolic Composites by a Compromising Porous Structure for Thermal Insulation

Investigations on effect of pore architectures of additively manufactured novel hydroxyapatite coated PLA/Al₂O₃ composite scaffold for bone tissue engineering

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Experimental measurement and simulation of mechanical strength and biological behavior of porous bony scaffold coated with alginate-hydroxyapatite for femoral applications

Cited by 21 publications

References 55 publications

Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering

Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering

Co-Optimizing Insulative and Mechanical Properties of Quartz Fabric Reinforced Phenolic Composites by a Compromising Porous Structure for Thermal Insulation

Investigations on effect of pore architectures of additively manufactured novel hydroxyapatite coated PLA/Al2O3 composite scaffold for bone tissue engineering

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Investigations on effect of pore architectures of additively manufactured novel hydroxyapatite coated PLA/Al₂O₃ composite scaffold for bone tissue engineering