Experimental models in peritoneal dialysis: A European experience

Lameire, Norbert; Biesen, Wim Van; Landschoot, Mieke Van; Wang, Tao; Heimbürger, Olof; Bergström, Jonas; Lindholm, Bengt; Hekking, Liesbeth P.H.; Havenith, Carin E.G.; Beelen, Robert H.J.

doi:10.1046/j.1523-1755.1998.00179.x

Cited by 53 publications

(55 citation statements)

References 65 publications

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“…The cumulative mortality, including the nephrectomy/sham procedure, was 0% (0/20) in controls versus 37.5% (9/24) in uraemic rats, in accordance with data published in the literature [16, 17]. About 70% of the mortality (7/9) occurred within 10 days, during or immediately after the two-step surgery: 3 rats in the VEGF-ab-treated uraemic group and 4 in the placebo-treated uraemic group.…”

Section: Resultssupporting

confidence: 89%

Pathophysiological Role of Vascular Endothelial Growth Factor in the Remnant Kidney

Schrijvers¹,

Flyvbjerg

Tilton

et al. 2005

Nephron Exp Nephrol

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Background: Subtotal renal ablation is characterized by initial glomerular hypertrophy, followed by progressive development of glomerulosclerosis and interstitial fibrosis. Vascular endothelial growth factor (VEGF) is involved in glomerular hypertrophy and dysfunction in several pathophysiological conditions. On the other hand, progressive glomerulosclerosis and tubulo-interstitial fibrosis in the remnant kidney have been associated with loss of VEGF expression. Methods: To explore the pathophysiological role of VEGF in the development of glomerular hypertrophy and renal damage in the remnant kidney model, we examined the effect of a neutralizing VEGF antibody on glomerular volume and kidney function in rats after subtotal nephrectomy or sham operation. Erythropoietin was administered to exclude a confounding effect of anaemia. Results: Six weeks after subtotal nephrectomy, plasma urea and creatinine concentrations, urinary albumin excretion, and mean glomerular volume were elevated in the placebo-treated uraemic rats as compared with the sham-operated rats. Inhibition of VEGF partially prevented the glomerular hypertrophy and largely prevented the rise in urinary albumin excretion, but did not affect creatinine clearance in uraemic rats. Conclusions: VEGF is a mediator of glomerular hypertrophy after subtotal renal ablation. In view of glomerular hypertrophy as the initial deleterious event ultimately leading to progressive glomerulosclerosis, agents that block this glomerular growth could be useful in preventing scarring in progressive renal disease.

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Section: Resultssupporting

confidence: 89%

Pathophysiological Role of Vascular Endothelial Growth Factor in the Remnant Kidney

Schrijvers¹,

Flyvbjerg

Tilton

et al. 2005

Nephron Exp Nephrol

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“…[1][2][3] There are many factors responsible for this malnutrition, and thus using an experimental animal model for nutritional pathophysiological research is effective. To date, several researchers have reported PD animal models [6,7] ; however, there have been few studies that have tried to clarify the effect of nutritional supply on these models, as most of these models were developed for dialysis assessment. We attempted to develop an appropriate PD model for evaluation of nutritional therapy including amino acid solutions.…”

Section: Discussionmentioning

confidence: 99%

New Peritoneal Dialysis Model in Rats with Bilateral Nephrectomy

et al. 2009

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Many peritoneal dialysis (PD) patients with chronic renal failure (CRF) suffer from metabolic and nutritional abnormalities. However, these abnormalities have been not sufficiently investigated. At present, the resolution of these issues in this field has been hindered by the lack of suitable PD models. We attempt to develop a rat model of PD under no constraints and under nonanesthetization to evaluate amino acid solution as suitable nutritional therapy for renal failure. In our model, bilateral nephrectomy rats were dialyzed 6 h per day for three days. The dialysate was infused and removed continually via a metering pump. Under fasting, rats were infused with 5% glucose or amino acid solution for renal failure, and they remained alive. This model can be used to examine bilateral nephrectomy in rats for three or more days. We were also able to determine protein and calorie malnutrition, negative nitrogen balance, abnormalities in the plasma amino acid pattern, and calcium and phosphorus metabolism. Thus, this model has the characteristics of renal failure in humans and may be used to easily examine the metabolic changes due to loss of kidney function.

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“…In parallel with clinical developments to reduce the impact of acute and chronic complications of PD, there has been a growing interest in establishing suitable experimental models to better understand the physiology and pathophysiology of fluid and solutes transport across the peritoneal membrane (PM). Thus far, most of the experimental studies of peritoneal transport have been performed in dog, rabbit, and rat models (reviewed in [1]). These studies yielded essential information on the kinetics of peritoneal transport and ultrafiltration (UF) in both normal and acute peritonitis conditions [2,3].…”

mentioning

confidence: 99%

“…Until recently, the body size of a species was considered as the major limiting factor to perform in vivo studies of PD [1]. This restriction is no longer applicable due to the availability of microsample analysis and molecular biology techniques, allowing the investigation of permeability parameters and multiple molecules at the mRNA and protein levels from minute volumes and tissue samples.…”

mentioning

confidence: 99%