Experimental Nuclear Medicine Meets Tumor Biology

Balber, Theresa; Tran, Loan; Benčurová, Katarína; Raitanen, Julia; Egger, Gerda; Mitterhauser, Markus

doi:10.3390/ph15020227

Cited by 6 publications

(5 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These imaging methods can be divided into two groups: morphological/anatomical imaging and molecular imaging based on nanobodies. Multidisciplinary collaboration across several domains, including radiology, nuclear medicine, pharmacology, chemistry, molecular and cell biology, physics, mathematics, and engineering, has led to the creation and translation of molecular imaging [ 5 , 13 , 14 , 15 , 16 , 17 ].…”

Section: Ultrasound (Us)mentioning

confidence: 99%

“…According to the World Health Organization, since cancer is positively correlated with patients’ ages, even if it affects younger people, cancer incidence prevalence and mortality are expected to significantly raise due to population growth and aging. This increases the importance of promoting and advancing health care systems and strategies and developing conventional therapy methods, including chemotherapy, radiation therapy, and surgery, to eliminate cancer cells, increase the survival rates of patients, and ensure sustainability, providing satisfactory global healthcare based on prevention, accurate diagnosis, and effective treatment that has less multi-drug resistance, high selectivity, and less cytotoxicity [ 4 , 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Conventional cancer therapy is non-targeting and damages healthy cells due to the accumulation of therapy on them, leading to the reduced efficiency of treatment. Therefore, it is desirable to develop targeted drug delivery systems that can deliver the therapeutic agent to the target tumor to reduce the adverse effects and improve efficacy [ 4 , 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Role of Molecular Imaging in Personalized Medicine

Salih

Elliyanti

Alkatheeri³

et al. 2023

JPM

View full text Add to dashboard Cite

The concept of personalized medicine refers to the tailoring of medical treatment to each patient’s unique characteristics. Scientific advancements have led to a better understanding of how a person’s unique molecular and genetic profile makes them susceptible to certain diseases. It provides individualized medical treatments that will be safe and effective for each patient. Molecular imaging modalities play an essential role in this aspect. They are used widely in screening, detection and diagnosis, treatment, assessing disease heterogeneity and progression planning, molecular characteristics, and long-term follow-up. In contrast to conventional imaging approaches, molecular imaging techniques approach images as the knowledge that can be processed, allowing for the collection of relevant knowledge in addition to the evaluation of enormous patient groups. This review presents the fundamental role of molecular imaging modalities in personalized medicine.

show abstract

Section: Ultrasound (Us)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Role of Molecular Imaging in Personalized Medicine

Salih

Elliyanti

Alkatheeri³

et al. 2023

JPM

View full text Add to dashboard Cite

show abstract

“…Multicellular tumor spheroids were found to better recapitulate in vivo tumors in terms of their structure and growth behavior. Their structure is characterized by an inner necrotic core, an adjacent quiescent zone, and a proliferating outer layer, establishing molecular gradients [14][15][16][17]. The phenomenon of hypoxia and, thereof, resulting necrosis was previously shown to be apparent in many in vivo tumors [18].…”

Section: Introductionmentioning

confidence: 99%

Radiobiological Assessment of Targeted Radionuclide Therapy with [177Lu]Lu-PSMA-I&T in 2D vs. 3D Cell Culture Models

Raitanen,

Barta,

Fuchs

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

In vitro therapeutic efficacy studies are commonly conducted in cell monolayers. However, three-dimensional (3D) tumor spheroids are known to better represent in vivo tumors. This study used [177Lu]Lu-PSMA-I&T, an already clinically applied radiopharmaceutical for targeted radionuclide therapy against metastatic castrate-resistant prostate cancer, to demonstrate the differences in the radiobiological response between 2D and 3D cell culture models of the prostate cancer cell lines PC-3 (PSMA negative) and LNCaP (PSMA positive). After assessing the target expression in both models via Western Blot, cell viability, reproductive ability, and growth inhibition were assessed. To investigate the geometric effects on dosimetry for the 2D vs. 3D models, Monte Carlo simulations were performed. Our results showed that PSMA expression in LNCaP spheroids was highly preserved, and target specificity was shown in both models. In monolayers of LNCaP, no short-term (48 h after treatment), but only long-term (14 days after treatment) radiobiological effects were evident, showing decreased viability and reproductive ability with the increasing activity. Further, LNCaP spheroid growth was inhibited with the increasing activity. Overall, treatment efficacy was higher in LNCaP spheroids compared to monolayers, which can be explained by the difference in the resulting dose, among others.

show abstract

“…Additional diagnostics often include imaging studies to assess the tumour stage, plan the treatment, and evaluate its effectiveness [ 13 , 14 ]. Nuclear molecular imaging is therefore an important complementary method to histopathological assessment [ 15 , 16 , 17 ]. Multimodal imaging using positron emission tomography (PET) coupled with magnetic resonance imaging (MRI) or computed tomography provides anatomical information and simultaneously allows for sensitive in vivo investigation of biological processes on the molecular level [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

CAM-Xenograft Model Provides Preclinical Evidence for the Applicability of [68Ga]Ga-Pentixafor in CRC Imaging

Benčurová

Friske

Anderla

et al. 2022

Cancers

Self Cite

View full text Add to dashboard Cite

Colorectal cancer is one of the leading causes of cancer-related deaths worldwide. Increased expression of CXCR4 has been associated with liver metastasis, disease progression, and shortened survival. Using in vitro cell binding studies and the in ovo model, we aimed to investigate the potential of [68Ga]Ga-Pentixafor, a radiotracer specifically targeting human CXCR4, for CRC imaging. Specific membrane binding and internalisation of [68Ga]Ga-Pentixafor was shown for HT29 cells, but not for HCT116 cells. Accordingly, [68Ga]Ga-Pentixafor accumulated specifically in CAM-xenografts derived from HT29 cells, but not in HCT116 xenografts, as determined by µPET/MRI. The CAM-grown xenografts were histologically characterised, demonstrating vascularisation of the graft, preserved expression of human CXCR4, and viability of the tumour cells within the grafts. In vivo viability was further confirmed by µPET/MRI measurements using 2-[18F]FDG as a surrogate for glucose metabolism. [68Ga]Ga-Pentixafor µPET/MRI scans showed distinct radiotracer accumulation in the chick embryonal heart, liver, and kidneys, whereas 2-[18F]FDG uptake was predominantly found in the kidneys and joints of the chick embryos. Our findings suggest that [68Ga]Ga-Pentixafor is an interesting novel radiotracer for CRC imaging that is worth further investigation. Moreover, this study further supports the suitability of the CAM-xenograft model for the initial preclinical evaluation of targeted radiopharmaceuticals.

show abstract

Experimental Nuclear Medicine Meets Tumor Biology

Cited by 6 publications

References 88 publications

The Role of Molecular Imaging in Personalized Medicine

The Role of Molecular Imaging in Personalized Medicine

Radiobiological Assessment of Targeted Radionuclide Therapy with [177Lu]Lu-PSMA-I&T in 2D vs. 3D Cell Culture Models

CAM-Xenograft Model Provides Preclinical Evidence for the Applicability of [68Ga]Ga-Pentixafor in CRC Imaging

Contact Info

Product

Resources

About