Experimental organ preservation

Abstract

SUMMARYAn experimental model for the study of organ preservation based on the isolated, biologically perfused rat heart is described. Using this model a standard storage solution has been evolved for use with initial perfusion and hypothermic storage. This solution contains Dextran 40 and insulin. Various perfusion characteristics including pH and infusion volume have also been studied. This solution has been modified and compared with two currently available storage solutions-Collins's C4 solution and the Gelin technique-and found to be superior to both. The technique consists of two parts; first, the organ is flushed with 0.5 per cent lignocaine in a buffered solution and is then perfused with the Dextran-40-based solution containing insulin and lignocaine. Further modification of the solution to contain a high magnesium concentration has resulted in a further improvement in survival after storage. These results are now being confirmed in the dog kidney.

Perfusion in the experimental preservation of the rat heart

“…Preservation of the rat heart has been extensively investigated by Calman and Bell (1972), They reported a convenient experimental model which enables detailed studies of preservation fluid constituents and conditions. Though the preserved heart does not function for long, the presence of strong contractions for 10 minutes after revascularization has been shown to be closely related to persistent function after transplantation (Calman and Bell, 1972). Each recipient animal may be used to test 5-10 hearts after preservation.…”

“…silastic tubing to the donor heart ( Fig. I) following the techniques of Calman and Bell (1972). The recipient's aorta was connected to the aortic cannula of the donor heart so as to perfuse the coronary vessels.…”

“…In this study the rat heart has been used in a preservation model previously reported by Calman and Bell (1972). Four types of perfusate have been studied.…”

Zur Ultrastruktur der Schweineleber bei 24stündiger einfacher hypothermer Konservierung

“…Zellstabilisatoren --Phenothiazinen und Glueocorticoiden --zur Perfusionsl6sung kommt nach neueren Untersuehungen fiir die Organkonservierung eine besondere Bedeutung zu [16,17,36,30,9,7]. Zellstabilisatoren --Phenothiazinen und Glueocorticoiden --zur Perfusionsl6sung kommt nach neueren Untersuehungen fiir die Organkonservierung eine besondere Bedeutung zu [16,17,36,30,9,7].…”