Experimental Research on Testing Methods of Innovating in vitro Grafting of Cherry (Prunus sp.)

Abstract

The experiments presented in this scienti ic paper were conducted with the aim of testing methods of innovating in vitro grafting of cherry (Prunus sp.), to obtain grafted vitroplants that can be used to establish ef icient orchards in Romania. The primary source of inoculum for the biological vegetable material was the apical vegetative cherry buds (Prunus sp.), for both rootstock varieties (Mazzard and Gisela 6) and scion varieties (Stella, Severin, Rubin). The experimental scheme included eighteen experimental variants carried depending on the grafting method, the rootstock variety and scion variety. The Gisela 6 rootstock variety allowed obtaining the highest number of grafted vitroplants with each of the three scion varieties, through both M3 method and M2 method. Also, the Rubin scion variety allowed obtaining the highest number of vitroplants grafted independently of the rootstock variety, thorough both M3 method and M2 method. The M3 method yielded the best experimental results for both grafted vitroplants consisting of Gisela 6 rootstock and Rubin scion and those consisting of Gisela 6 rootstock and Severin scion. The experimental variant that yielded the best experimental results consisted of method M3, Gisela 6 rootstock and Rubin scion. The results of the experimental research presented in this scienti ic paper demonstrates that testing methods of innovating in vitro grafting of cherry (Prunus sp.), is feasible to obtain grafted vitroplants that can be used to establish ef icient orchards in Romania.