Experimental respiratory syncytial virus infection of four species of primates

Abstract

Four species of nonhuman primates were inoculated intranasally with 10(3.1) to 10(3.7) plaque forming units (pfu) of respiratory syncytial (RS) virus. Adults squirrel monkeys and newborn rhesus monkeys became infected and shed small quantities (peak titer 10(2.0) pfu/ml of nasopharyngeal swab specimen) of virus, but illness did not develop. Infant cebus monkeys aged 2 months became infected, shed 10(2.3) to 10(3.8) pfu/ml of nasopharyngeal swab specimen, but did not become ill. Chimpanzees aged 15 to 18 months shed a large quantity of virus, up to 10(6.0) pfu/ml of nasopharyngeal swab specimen and developed an upper respiratory illness. Chimpanzees are proposed as a possible animal model for future study of the immunopathology of RS virus disease and for in vivo evaluation of attenuated live virus vaccine candidates.

Efficacy of RD3-0028 aerosol treatment against respiratory syncytial virus infection in immunosuppressed mice

“…In vivo evaluation of RSV-infected animals has been carried out with the cotton rat model in previous studies (10,21,22,31). In addition, an experimental model with ferrets (24) and primates (1,25) has been reported, and Graham et al (8) found that CYP-untreated, 8-to 10-month-old mice were more susceptible to RSV infection than 8-week-old mice. However, other than the cotton rat, the animal models reported previously are much more difficult to use for an antiviral drug evaluation.…”

Identification of Five Different Patr Class I Molecules That Bind HLA Supertype Peptides and Definition of Their Peptide Binding Motifs

“…Chimpanzees are closely related to humans from an evolutionary standpoint. As such, they can be experimentally infected with a number of human viral pathogens, such as hepatitis B and C viruses (HBV and HCV) 3 (1, 2), HIV (3), and Rous sarcoma virus (4), as well as bacterial and protozoal infections, such as leprosy and tuberculosis (5). Some viral infections, such as HBV, follow a clinical course very similar to the human disease (1).…”

Mouse Model of Respiratory Syncytial Virus Infection to Evaluate Antiviral Activity in Vivo

“…In addition, in vivo evaluation using ferrets (Prince & Porter, 1976) and primates (Belshe et al, 1977) has been reported. However, animals other than the cotton rat are much more difficult to use for antiviral drug evaluation.…”