Experimental Retinal Detachment

Pederson, Jonathan E.

doi:10.1001/archopht.1985.01050060095033

Cited by 16 publications

(2 citation statements)

References 13 publications

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“…Since Machemer's original investigation, new models have been devised and modified by other researchers, but fluid was always injected into the subretinal space. For instance, 0.25% sodium hyaluronate was used in the experiment of Cook et al [2] and Anderson et al [3], while Ringer solution was injected into the subretinal space by Pederson et al [4]. Injection of hyaluronate is not entirely satisfactory for experimental investigations, as this approach usually changes the chemistry of mucopolysaccharides in the interreceptor space.…”

Section: Introductionsupporting

confidence: 40%

“…Each of the eyeballs was then dissected by means of a razor blade into an anterior and a posterior half. The anterior half with the lens was discarded and the posterior half was incubated for 5 min at 37°C in 1 ml of MEM solution with 1 ÌCi labeled leucine (L- [4, H]leucine, code TRK.636, Amersham). This timing was selected, as preliminary studies demonstrated optimal uptake of leucine with this timing.…”

Section: Scintillation Countingmentioning

confidence: 47%

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Changes in Leucine Uptake in the Retina of the Hamster after Traumatic Detachment

Yau

Li²,

Yew³

2000

Biol Signals Recept

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Protein metabolism was investigated in detached hamster retinas. By sucking off 0.2 ml of aqueous humor from the anterior chamber through limbic insertion of a 27-gauge needle, a tractional force pulled off the neural retina from the retinal pigment epithelium and created a simple detachment without retinal breaks in the right eyes of the hamsters. The left eyes were left untouched as normal controls and sham controls were induced by simple limbic insertion without suction. The animals were sacrificed at selected intervals of 1, 3, 6, 9, 16, 24, 32 days after the operation. Subsequently, scintillation counting and autoradiography were employed to study retinal protein metabolism using leucine uptake as an index. After tritiated leucine uptake, scintillation counting of radioactive substance indicated that detached retinas had taken in less tritiated leucine than normal controls from day 1 to 6 after the operation, but this change had normalized by day 9. For autoradiography, the change in leucine uptake rate was shown to be different in different layers. All the retinal cells seemed to show a decreased leucine uptake with the exception of the outer nuclear layer, in which leucine appeared to be significantly upregulated. This paper illustrates the patterns of protein metabolism and their change after traumatic detachment as well as their possible recovery.

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Section: Introductionsupporting

confidence: 40%

Section: Scintillation Countingmentioning

confidence: 47%