Experimental study of a mixed vaccine against peste des petits ruminants and capripox infection in goats in northern Cameroon

Martrenchar, Arnaud; Zoyem, N.; Diallo, Adama

doi:10.1016/s0921-4488(96)00989-3

Cited by 28 publications

(17 citation statements)

References 15 publications

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“…The role of PPRV for suppressing the immune system of the goats should not be ignored in this case. A few cases of mixed viral infections in sheep and goats involving PPRV and orf virus or goat pox virus or BTV have been reported in India and other countries (Mehrotra et al 1995;Martrenchar et al 1997;Saravanan et al 2007). Morbilliviruses in general have been shown to cause suppression of host immune responses (Yanagi et al 1992;Wohlsein et al 1995).…”

Section: Discussionmentioning

confidence: 99%

Evidence of mixed infection of peste des petits ruminants virus and bluetongue virus in a flock of goats as confirmed by detection of antigen, antibody and nucleic acid of both the viruses

Mondal

Chand

Biswas

et al. 2009

Trop Anim Health Prod

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A mixed infection with peste des petits ruminants virus (PPRV) and bluetongue virus (BTV) occurred in goats which exhibited symptoms characteristic of PPR. A number of samples were collected from ailing or dead goats for labrotory diagnosis. Antibody to BTV and PPRV was detected in sera samples by competitive ELISA. No PPRV antigen was detected in tissue samples like lung and spleen, however, presence of PPRV antigen in some sera samples was confirmed by sandwich ELISA. All the blood samples collected from the ailing animals were found positive for BTV antigen by a sandwich ELISA. BTV- and PPRV nucleic acids were amplified from the pooled blood and tissue samples respectively by RT-PCR assays. The identity of the amplicons was confirmed by cloning and sequencing. All these tests confirm that the goats were infected with PPRV and BTV simultaneously. Isolation of viruses from the clinical samples is underway.

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Section: Discussionmentioning

confidence: 99%

Evidence of mixed infection of peste des petits ruminants virus and bluetongue virus in a flock of goats as confirmed by detection of antigen, antibody and nucleic acid of both the viruses

Mondal

Chand

Biswas

et al. 2009

Trop Anim Health Prod

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show abstract

“…To protect goats from PPR and Goat Pox (GP), a combined live attenuated PPR and Capripox virus vaccine of sheep origin had been used in Cameroon [91]. Further, considering the similar geographic distribution of both PPR and goat or sheep pox virus infection as well as occurrence of mixed infections due to these viruses, use of a combined vaccine for control of these infections particularly in the endemic areas will be a good option.…”

Section: Prevention and Controlmentioning

confidence: 99%

Diagnosis and control of peste des petits ruminants: a comprehensive review

Balamurugan¹,

Hemadri²,

Gajendragad³

et al. 2013

VirusDis.

135

118

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Peste des petits ruminants (PPR) is an acute, highly contagious, world organization for animal health (OIE) notifiable and economically important transboundary viral disease of sheep and goats associated with high morbidity and mortality and caused by PPR virus. PPR is considered as one of the main constraints in augmenting the productivity of small ruminants in developing countries and particularly severely affects poor farmer's economy. The disease is clinically manifested by pyrexia, oculo-nasal discharges, necrotizing and erosive stomatitis, gastroenteritis, diarrhoea and bronchopneumonia. The disease can be diagnosed from its clinical signs, pathological lesions, and specific detection of virus antigen/antibodies/genome in the clinical samples by various serological tests and molecular assays. PPR is the one of the priority animal diseases whose control is considered important for poverty alleviation in enzootic countries. Availability of effective and safe live attenuated cell culture PPR vaccines and diagnostics have boosted the recently launched centrally sponsored control programme in India and also in other countries. This review article primarily focus on the current scenario of PPR diagnosis and its control programme with advancement of research areas that have taken place in the recent years with future perspectives.

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“…The combined results, based on 98,000 sheep and goats of which 58,000 were vaccinated, indicated that approximately 98 % of the vaccinated animals seroconverted after 1 month and remained protective for at least 3 years. The effective dose was calculated to be 10 0.8 TCID50/animal; however, a dose of 10 3 TCID50/animal also proved to be safe (Martrenchar et al 1997). Pregnant animals remained safe and were able to pass passive immunity to their offspring, which remained protected for 3-5 months.…”

Section: Homologous Attenuated Pprv Vaccinesmentioning

confidence: 98%

“…One such effort was made in Cameroon when 20 goats were immunized with a mixed vaccine carrying attenuated PPRV 75/l strain for PPR and the RM 65 strain for capripoxvirus, and then challenged with wild virulent strains of PPR and goat pox (Martrenchar et al 1997). Unfortunately, the RM 65 strain did not act as an effective vaccine against the virulent field strain of goat pox used in the challenge experiment.…”

Section: Multivalent Vaccinesmentioning

confidence: 99%

“…Unfortunately, the RM 65 strain did not act as an effective vaccine against the virulent field strain of goat pox used in the challenge experiment. The authors speculated that the failure of these vaccines could be due to a lack of cross protection between the RM 65 strain and the challenged wild goat pox strain, or due to the RM 65 vaccine adaptation to the Vero cell culture (Martrenchar et al 1997). Based on these observations, and partial protection between sheep and goat pox viruses, a homologous vaccine was recommended for full protection .…”

Section: Multivalent Vaccinesmentioning

confidence: 99%

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Current Advances in Molecular Diagnosis and Vaccines for Peste des Petits Ruminants

Munir

Zohari²,

Berg

2012

Molecular Biology and Pathogenesis of Peste Des Petits Ruminants Virus

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There has been a substantial improvement in the detection of the nucleic acids of Peste des Petits Ruminants Virus (PPRV), and effective vaccines have been developed in recent years. Demonstrations of several real-time PCR assays have provided powerful and novel means of not only detection but also quantification of the nucleic acids of PPRV in several types of clinical samples. Although most of the lineages are continent specific, reports on mixed lineages are emerging, such as in Sudan and Uganda. None of the available assays is devised so far to differentiate all of the lineages. Despite essential advances in the marker vaccines, it is still required to establish reverse genetics systems to rescue recombinant PPRV vaccines, primarily by manipulating the genes of PPRV and insertion of positive or negative markers, which ultimately will lead to the development of companion test. Similarly, swapping different genes will facilitate the establishment of a test that can differentiate vaccinated and infected animals. In this chapter, we discuss available diagnostic tests and potent PPRV vaccines. Furthermore, the current advances in both fields and possibilities to develop next-generation assays and vaccines for PPRV are critically discussed.

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Experimental study of a mixed vaccine against peste des petits ruminants and capripox infection in goats in northern Cameroon

Cited by 28 publications

References 15 publications

Evidence of mixed infection of peste des petits ruminants virus and bluetongue virus in a flock of goats as confirmed by detection of antigen, antibody and nucleic acid of both the viruses

Evidence of mixed infection of peste des petits ruminants virus and bluetongue virus in a flock of goats as confirmed by detection of antigen, antibody and nucleic acid of both the viruses

Diagnosis and control of peste des petits ruminants: a comprehensive review

Current Advances in Molecular Diagnosis and Vaccines for Peste des Petits Ruminants

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