Experimental study of non-alcoholic fatty liver disease (NAFLD) on a model of starving chickens: Is generalization of steatosis accompanied by fibrosis of the liver tissue?

Makovický, Peter; Dudova, Marketa; Tůmová, E.; Rajmon, Radko; Vodková, Z.

doi:10.1016/j.prp.2010.12.002

Cited by 20 publications

(8 citation statements)

References 51 publications

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“…On the other hand, Makovický et al (2011) demonstrated that early feed restriction (between the 8 th and 14 th day of age) in broiler chickens decreased hepatocyte diameter. This reduction of hepatocyte size was associated with induced steatosis in chickens (Makovický et al, 2011) and rats (Grattagliano et al, 2000). It is possible to assume that restricted feeding does not cause steatosis or affect liver function in rabbits, which can reveal GMT concentration.…”

Section: Digestibility Of Nutrientsmentioning

confidence: 97%

The effect of 1-week feed restriction on performance, digestibility of nutrients and digestive system development in the growing rabbit

Tůmová

Volek

Chodová

et al. 2016

Animal

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A 3 to 4 week feed restriction of about 20% to 25% of the free intake is widely applied in rabbit breeding systems to reduce postweaning digestive disorders. However, a short intensive feed restriction is described in few studies and can be beneficial for growing rabbits due to a longer re-alimentation period. The aim of this study was to evaluate the effect of ad libitum (AL) and two restriction levels of feeding (50 and 65 g/rabbit per day) applied for 1 week on performance, gastrointestinal morphology and physiological parameters during the restriction and during the re-alimentation period. Rabbits were divided into three experimental groups: AL rabbits were fed AL, R1 rabbits were restricted from 42 to 49 days of age and received 50 g daily (29% of AL) and R2 rabbits were restricted at the same age and were fed 65 g of feed daily (37% of AL). In the 1 st week after weaning and in the weeks after restriction, all the groups were fed AL. During the restriction period, daily weight gain (DWG) in R1 significantly dropped to 11% (experiment 1) and 5% (experiment 2) compared with rabbits in the AL group, although they were fed 29% of AL, whereas in the R2 group it decreased to 20% (experiment 1) and 10% (experiment 2). In the week following feed restriction, DWG in the restricted groups increased (P < 0.001) to 166% and 148% in R1 and to 128% and 145% in R2. Restricted rabbits in both the experiments reached up to 90% to 93% of the final live weight (70 days) of the AL group. Over the entire experimental period, feed restriction significantly decreased feed intake to 85% to 88% of the AL group; however, the feed conversion ratio was lower (P < 0.05) only in experiment 1 (−6% in R1 and −4% in R2). Digestibilities of CP and fat were not significantly higher during the restriction period and during the 1 st week of re-alimentation compared with the AL group. Significant interactions between feeding regime and age revealed the shortest large intestine in the AL group at 49 days of age and the longest at 70 days in the AL and R1 groups. Small intestinal villi were significantly higher and the crypts were significantly deeper in the restricted groups. It could be concluded that short intensive feed restriction increased digestible area in the small and large intestines, including the height of villi and depth of crypts, which might be involved in the compensatory growth and defence mechanism.

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Section: Digestibility Of Nutrientsmentioning

confidence: 97%

The effect of 1-week feed restriction on performance, digestibility of nutrients and digestive system development in the growing rabbit

Tůmová

Volek

Chodová

et al. 2016

Animal

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“…In rodents and rabbits, the adipose tissues and liver provide the equal function of de novo lipogenesis [ 8 , 9 ]. Therefore, the chicken have been suggested and used as an animal model for human liver diseases recently [ 10 – 12 ]. Although several studies described that the excessive hepatic lipogenesis and lipid accumulation are the very initiator of the liver fibrosis [ 2 ], liver fibrosis can also be a result of other liver injuries.…”

Section: Introductionmentioning

confidence: 99%

Alleviation of Carbon-Tetrachloride-Induced Liver Injury and Fibrosis by Betaine Supplementation in Chickens

Tsai

Chen

Pan

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Betaine is a food component with well-reported hepatoprotection effects. However, the effects and mechanisms of betaine on liver fibrosis development are still insufficient. Because metabolic functions of chicken and human liver is similar, we established a chicken model with carbon Tetrachloride- (CCl4-) induced fibrosis for studying antifibrotic effect of betaine in vivo and in vitro. Two-week-old male chicks were supplemented with betaine (1%, w/v) in drinking water for 2 weeks prior to the initiation of CCl4 treatment (i.p.) until sacrifice. Primary chicken hepatocytes were treated with CCl4 and betaine to mimic the in vivo supplementation. The supplementation of betaine significantly alleviated liver fibrosis development along with the inhibition of lipid peroxidation, hepatic inflammation cytokine, and transforming growth factor-β1 expression levels. These inhibitive effects were also accompanied with the attenuation of hepatic stellate cell activation. Furthermore, our in vitro studies confirmed that betaine provides antioxidant capacity for attenuating the hepatocyte necrosis by CCl4. Altogether, our results highlight the antioxidant ability of betaine, which alleviates CCl4-induced fibrogenesis process along with the suppression of hepatic stellate cells activation. Since betaine is a natural compound without toxicity, we suggest betaine can be used as a potent nutritional or therapeutic factor for reducing liver fibrosis.

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“…In order to better understand hepatic fibrosis and to choose the best experimental model it is also important to explore the evolutionary aspects of fibrosis. Mammalian models are used most often and consequently there is little information regarding hepatic fibrosis in non-mammalian models (Bunton, 1995;Ochiai et al, 2003;Rekha et al, 2008;Makovicky et al, 2011;Hobbie et al, 2012;Van Wettere et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Evidence for Myofibroblast-like Cells Associated with Liver Injury Induced by Aflatoxin B1 in Rainbow Trout (Oncorhynchus mykiss)

Arana

Alves

Sabino

et al. 2014

Journal of Comparative Pathology

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Experimental study of non-alcoholic fatty liver disease (NAFLD) on a model of starving chickens: Is generalization of steatosis accompanied by fibrosis of the liver tissue?

Cited by 20 publications

References 51 publications

The effect of 1-week feed restriction on performance, digestibility of nutrients and digestive system development in the growing rabbit

The effect of 1-week feed restriction on performance, digestibility of nutrients and digestive system development in the growing rabbit

Alleviation of Carbon-Tetrachloride-Induced Liver Injury and Fibrosis by Betaine Supplementation in Chickens

Immunohistochemical Evidence for Myofibroblast-like Cells Associated with Liver Injury Induced by Aflatoxin B1 in Rainbow Trout (Oncorhynchus mykiss)

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