Experimental Study of the Tendon Healing and Remodeling After Local Injection of Bone Marrow Myeloid Tissue in Rabbit

Naseri, Fatemeh; SHahri, Nasser Mahdavi; Sardari, Kamran; Moghimi, Ali; Bahrami, Ahmad Reza; Kheirabadi, Masoumeh; Edalatmanesh, Mohammad Amin

doi:10.3923/jbs.2008.591.597

Cited by 2 publications

(4 citation statements)

References 38 publications

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“…4 ). This result is in line with previous studies using the same animal model 14 15 16 17 18 , in which the collagenase produced tendon damage characterised by loss of arrangement of collagen fibres, increased cell density and rounding of the nuclei of the tenocytes, amongst others 14 15 16 17 18 . In contrast, the group of tendons treated with LR-PRP showed a general histology score not statistically different to the tendons of the control healthy group ( Fig.…”

Section: Discussionsupporting

confidence: 92%

“…Firstly, although an animal model of TAT has served to evaluate the evolution of this disease and its response to either the LR-PRP or placebo treatment, the model does not reflect the conditions of naturally occurring TAT. Nevertheless, this is still a widely-accepted model of TAT, because lesions induced by collagenase mimic tendon injury and they are reproducible with a consistent histological lesion 14 15 16 17 18 . One additional limitation of this study was the short time period of experimental evaluation.…”

Section: Discussionmentioning

confidence: 99%

“…The rabbit model of TAT induced by collagenase has been employed for evaluating the effects of several drugs or procedures (either experimental or clinical) used for the treatment of this disease 14 15 16 17 18 . However, to the knowledge of the authors, no studies exist which evaluate the effect of PRP in this animal model of tendinopathy.…”

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confidence: 99%

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Autologous leukocyte-reduced platelet-rich plasma therapy for Achilles tendinopathy induced by collagenase in a rabbit model

Gonzalez

López

Álvarez

et al. 2016

Sci Rep

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Leukocyte-reduced platelet-rich plasma (LR-PRP) is a therapy for tendinopathy of the Achilles tendon (TAT); however, there is scarce information regarding LR-PRP effects in rabbit models of TAT. We compared, at 4 and 12 weeks (w), the LR-PRP and placebo (PBS) effects on ultrasonography, histology and relative gene expression of collagen types I (COL1A1) and III (COL3A1) and vascular endothelial growth factor (VEGF) in 24 rabbits with TAT induced by collagenase. The rabbits (treated with both treatments) were euthanatised after either 4 or 12 w. A healthy group (HG (n = 6)) was included. At 4 and 12 w, the LR-PRP group had a no statistically different histology score to the HG. At w 4, the COL1A1 expression was significantly higher in the LR-PRP group when compared to HG, and the expression of COL3A1from both LR-PRP and PBS-treated tendons was significantly higher when compared to the HG. At w 12, the expression of COL3A1 remained significantly higher in the PBS group in comparison to the LR-PRP group and the HG. At w 4, the LR-PRP group presented a significantly higher expression of VEGF when compared to the PBS group and the HG. In conclusion, LR-PRP treatment showed regenerative properties in rabbits with TAT.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Autologous leukocyte-reduced platelet-rich plasma therapy for Achilles tendinopathy induced by collagenase in a rabbit model

Gonzalez

López

Álvarez

et al. 2016

Sci Rep

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show abstract

“…Tendon specimens collected at each time point of the study were immediately fixed in 10% buffered formalin, routinely processed, sectioned at 6 μm and stained with Hematoxylin and Eosin (H&E) as well as Masson’s trichrome [26] . Each specimen of treatment group were histomorphologically analyzed qualitatively using the following parameters: vascularization, cellularity, collagen fibers alignment, inflammatory cells and granulation tissues [27] . Histomorphological scores are shown in Table 2 .…”

Section: Methodsmentioning

confidence: 99%

Clinical and biomechanical evaluation of three bioscaffold augmentation devices used for superficial digital flexor tenorrhaphy in donkeys (Equus asinus): An experimental study

El-Shafaey

Karrouf

Zaghloul

2013

Journal of Advanced Research

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The present study was designed to carry out an in vivo and in vitro comparative evaluation of three bio-scaffold augmentation devices used for superficial digital flexor tenorrhaphy in donkeys. Twenty-four clinically healthy donkeys were assigned for three treatment trials (n = 8) using one of three bioscaffold materials (glycerolized bovine pericardium xenograft, tendon allograft and allograft with glycerolized by bovine pericardium). In addition, eight clinically healthy donkeys were selected to serve as control. Clinical signs of each animal were scored and the sum of all clinical indexes was calculated at each time point of the experiment. Four donkeys from each group were euthanized at 45 and 90 days postoperatively, respectively, for biomechanical and histopathological evaluation of treated superficial digital flexor tendon (SDFT). The failure stress in allograft shielding group significantly increased compared to the corresponding values of the other groups at 45 (62.7 ± 6.5 N mm−2) and 90 (88.8 ± 3.5 N mm−2) days postoperatively. The fetlock angle in the allograft shielding group at both 45 (112.8° ± 4.4) and 90 (123.8° ± 1.1) days postoperatively showed a significant increase (p < 0.05) relative to the values of the other groups and a significant decrease (p < 0.05) when compared to normal angle (125° ± 0). However, the histomorphological findings revealed no remarkable changes between the treatment groups. In conclusion, the failure stress, fetlock angle and histomorphological findings may provide useful information about the healing characteristics of SDFT tenorrhaphy. The bio-scaffold augmentation devices, either xenogenic or allogenic, provide good alternative techniques accelerating SDFT healing with minimal adhesions in donkeys.

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Experimental Study of the Tendon Healing and Remodeling After Local Injection of Bone Marrow Myeloid Tissue in Rabbit

Cited by 2 publications

References 38 publications

Autologous leukocyte-reduced platelet-rich plasma therapy for Achilles tendinopathy induced by collagenase in a rabbit model

Autologous leukocyte-reduced platelet-rich plasma therapy for Achilles tendinopathy induced by collagenase in a rabbit model

Clinical and biomechanical evaluation of three bioscaffold augmentation devices used for superficial digital flexor tenorrhaphy in donkeys (Equus asinus): An experimental study

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