Experimental study of therapeutic efficacy of Baicalin in rats with severe acute pancreatitis

Zhang, Xiping; Zhang, Ling; He, Jin; Zhang, Ruiping; Cheng, Qi; Zhou, Yifeng; Liu, Bei

doi:10.3748/wjg.v13.i5.717

Cited by 33 publications

(47 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ten minutes after successful modeling, Baicalintreated group was first injected 5% Baicalin injection (10 mg/100 g) via the external jugular vein, followed by continuous intravenous administration (10 mg/h per 100 g) by microinfusion pump. Octreotide-treated group was first injected octreotide (0.2 μg/100 g) via the external jugular vein, followed by continuous intravenous transfusion (10 mg/h per 100 g) by microinfusion pump at a transfusion speed of 0.2 μg/h per 100 g. All abovementioned dosages have been proved as effective dosages in the previous preliminary experiment [10] . Both the sham operation group and model control group were injected normal saline of equivalent volume at the corresponding time points after operation.…”

Section: Dosagementioning

confidence: 96%

“…The improved Aho's method [10] was adopted to prepare 135 SAP rat models via retrograde injection of 35 g/L sodium taurocholate to the pancreatic duct through epidural catheter and duodenal papilla. The 135 SAP rat models were randomly assigned to the model group, Baicalintreated group and octreotide-treated group, 45 rats in each group, while other 45 rats were assigned to the sham operation group (SO group).…”

Section: Preparation Of Animal Modelsmentioning

confidence: 99%

“…The large dose (10 mg/h per 100 g) can achieve the best therapeutic effect and the dosage referred to the result of the previous preliminary experiment [10] . Ten minutes after successful modeling, Baicalintreated group was first injected 5% Baicalin injection (10 mg/100 g) via the external jugular vein, followed by continuous intravenous administration (10 mg/h per 100 g) by microinfusion pump.…”

Section: Dosagementioning

confidence: 99%

See 2 more Smart Citations

Protective effects and mechanisms of Baicalin and octreotide on renal injury of rats with severe acute pancreatitis

Zhang

Tian²,

Lai³

et al. 2007

WJG

Self Cite

View full text Add to dashboard Cite

AIM:To i n v e s t i g a t e t h e p r o t e c t i v e e f fe c t s a n d mechanisms of Baicalin and octreotide on renal injury of rats with severe acute pancreatitis (SAP). METHODS:One hundred and eighty SD rats were randomly assigned to the model group, Baicalin-treated group, octreotide-treated group and sham operation group. The mortality, plasma endotoxin level, contents of blood urea nitrogen (BUN), creatinine (CREA), phospholipase A2 (PLA2), nitrogen monoxide (NO), tumor necrosis factor (TNF)-α, IL-6 and endothelin-1 (ET-1) in serum, expression levels of renal Bax and Bcl-2 protein, apoptotic indexes and pathological changes of kidney were observed at 3, 6 and 12 h after operation. RESULTS:The renal pathological changes were milder in treated group than in model group. The survival at 12 h and renal apoptotic indexes at 6 h were significantly (P < 0.05) higher in treated group than in model group [66.67% vs 100%; 0.00 (0.02)% and 0.00 (0.04)% vs 0.00 (0.00)%, respectively]. The serum CREA content was markedly lower in octreotide-treated group than in model group at 3 h and 6 h (P < 0.01, 29.200 ± 5.710 μmol/L vs 38.400 ± 11.344 μmol/L; P < 0.05, 33.533 ± 10.106 μmol/L vs 45.154 ± 17.435 μmol/L, respectively).The expression level of renal Bax protein was not significantly different between model group and treated groups at all time points. The expression level of renal Bcl-2 protein was lower in Baicalin-treated group than in model group at 6 h [P < 0.001, 0.00 (0.00) grade score vs 3.00 (3.00) grade score]. The Bcl-2 expression level was lower in octreotide-treated group than in model group at 6 h and 12 h [P < 0.05, 0.00 (0.00) grade score vs 3.00 (3.00) grade score; 0.00 (0.00) grade score vs 0.00 (1.25) grade score, respectively]. The serum NO contents were lower in treated groups than in model group at 3 h and 12 h [P < 0.05, 57.50 (22.50) and 52.50 (15.00) μmol/L vs 65.00 (7.50) μmol/L; P < 0.01, 57.50 (27.50) and 45.00 (12.50) μmol/L vs 74.10 (26.15) μmol/L, respectively]. The plasma endotoxin content and serum BUN content (at 6 h and 12 h) were lower in treated groups than in model group. The contents of IL-6, ET-1, TNF-α (at 6 h) and PLA2 (at 6 h and 12 h) were lower in treated groups than in model group [P < 0.001, 3.031 (0.870) Key words: S e ve re a c u t e p a n c re a t i t i s ; B a i c a l i n ; Octreotide; Renal injury; Rats; Tissue microarrays

show abstract

Section: Dosagementioning

confidence: 96%

Section: Preparation Of Animal Modelsmentioning

confidence: 99%

Section: Dosagementioning

confidence: 99%

See 1 more Smart Citation

Protective effects and mechanisms of Baicalin and octreotide on renal injury of rats with severe acute pancreatitis

Zhang

Tian²,

Lai³

et al. 2007

WJG

Self Cite

View full text Add to dashboard Cite

show abstract

“…Once complicated by multiple organ injury, the mortality rate of SAP is greatly increased [23,24]. When SAP develops, because the release of a variety of inflammatory mediators runs out of control, a cascade-like reaction is triggered.…”

Section: Discussionmentioning

confidence: 99%

Influence of dexamethasone on the expression levels of P-selectin protein in multiple organs of rats with severe acute pancreatitis

Zhang

et al. 2009

Inflamm. Res.

View full text Add to dashboard Cite

show abstract

“…The rats were given food and water ad libitum and fasted overnight before experiments. Rat SAP models were prepared by the method of Zhang et al (2007). The SAP rats were slowly injected 2 ml/kg body weight through the tail vein of either SPIO-liposomes (P group), SPIO-clodronateliposomes (T group), or saline (C group).…”

Section: Animal Models and Experimental Groupingmentioning

confidence: 99%

Clodronate-superparamagnetic iron oxide-containing liposomes attenuate renal injury in rats with severe acute pancreatitis

Dang

Zeng

Wang

et al. 2014

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Background and objective: It has been shown that macrophages play an important role in the development of severe acute pancreatitis (SAP), and eventually lead to multiple organ failure (MOF). Clodronate-liposome selectively depleted macrophages. This study was to investigate the role of renal macrophage infiltration in acute renal injury in rats with SAP and to evaluate the potential of superparamagnetic iron oxide (SPIO)-enhanced magnetic resonance imaging (MRI) for diagnosis. Methods: Superparamagnetic Fe 3 O 4 nanoparticles were prepared by chemical coprecipitation. SPIO-liposomes and SPIO-clodronate-liposomes were prepared by the thin film method. SAP models were prepared by injection of sodium taurocholate into the subcapsular space of rat pancreas. Sprague-Dawley rats were randomly divided into a control group, SAP plus SPIO-liposome (P) group, and SAP plus SPIO-clodronatecontaining liposome (T) group. Kidney injury was evaluated by T2-weighted MRI scan. The levels of serum amylase (SAM), blood urea nitrogen (BUN), and serum creatinine (SCr) were measured by an automated enzymatic method. Serum tumor necrosis factor-α (TNF-α) was measured by enzyme-linked immunosorbent assay (ELISA). Pathological changes in the pancreas and kidney were observed using hematoxylin and eosin (H&E) staining, while cell apoptosis was detected with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. In addition, the macrophage markers (CD68) of the renal tissue were detected with immunohistochemistry. Results: The pathological changes in the pancreas and kidneys of rats in the T group were milder than those in the P group. The MRI signal intensity of the kidneys in the P and T groups was significantly lower than that in the control group. There were significant changes in the two experimental groups (P<0.01). The levels of SAM, Bun, SCr, and TNF-α in rats in the P group were higher than those in the control group (P<0.01) and in the T group (P<0.01). The apoptosis of the kidney in the T group was higher than that in the P group at 2 and 6 h (P<0.01). Conclusions: Clodronate-containing liposomes protected against renal injury in SAP rats, and SPIO can be used as a tracer for MRI examination to detect renal injury in SAP rats. SPIO-aided MRI provided an efficient non-invasive way to monitor the migration of macrophages after renal injury in rats with SAP.

show abstract

Experimental study of therapeutic efficacy of Baicalin in rats with severe acute pancreatitis

Cited by 33 publications

References 30 publications

Protective effects and mechanisms of Baicalin and octreotide on renal injury of rats with severe acute pancreatitis

Protective effects and mechanisms of Baicalin and octreotide on renal injury of rats with severe acute pancreatitis

Influence of dexamethasone on the expression levels of P-selectin protein in multiple organs of rats with severe acute pancreatitis

Clodronate-superparamagnetic iron oxide-containing liposomes attenuate renal injury in rats with severe acute pancreatitis

Contact Info

Product

Resources

About