Experimental study on allografts of amniotic epithelial cells in calcaneal tendon lesions of sheep

Muttini, Aurelio; Mattioli, Mauro; Petrizzi, Lucio; Varasano, Vincenzo; Sciarrini, Chiara; Russo, Valentina; Mauro, Annunziata; Cocciolone, D.; Turriani, Maura; Barboni, Barbara

doi:10.1007/s11259-010-9396-z

Cited by 23 publications

(27 citation statements)

References 5 publications

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“…There was sharp decrease in the number of inflammatory cells at 14 days in group T, while this decrease was slower in group I, similar to the results observed by Muttini et al 13 . These findings may be related to the anti-inflammatory properties of hAM cited in the study of Manuelpillai et al 10 .…”

Section: Barboni Et Alsupporting

confidence: 90%

“…In addition to these biological properties, others such as biocompatibility, permeability, highly flexibility, transparency, and stability make the MA a tissue with reasonable mechanical properties and, thus a potential scaffold the growth, cell adhesion and migration, characteristics required for tissue engineering 6 . Pre-clinical studies have successfully used the amniotic epithelial cells (AECs) [12][13] to accelerate the repair process in tendinopathies. However, cell culture can result in a loss of some of its own cellular factors, such as cytokines as well as growth and differentiation factors, which are present in the AM microenvironment.…”

Section: ■ Introductionmentioning

confidence: 99%

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Amniotic membrane as an option for treatment of acute Achilles tendon injury in rats

et al. 2017

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1 5-Experimental SurgeryActa Cir Bras. 2017;32(2):125-139 Abstract Purpose: To evaluate the effect of human amniotic membrane (hAM) fragment on inflammatory response, proliferation of fibroblast and organization of collagen fibers in injured tendon. Methods: Sixty rats were divided into 3 groups: C -surgical procedures without tendon lesion and with simulation of hAM application; I -surgical procedures, tendon injury and simulation of hAM application; T -surgical procedures, tendon injury and hAM application. These groups were subdivided into four experimental times (3, 7, 14 and 28 days). The samples underwent histological analysis and ATR-FTIR spectroscopy. Results: Histological analysis at 14 days, the T group showed collagen fibers with better alignment. At 28 days, the I group presented the characteristics described for the T group at 14 days, while this group presented aspects of a mature connective tissue. FT-IR analysis showed a clear distinction among the three groups at all experimental times and groups T and I presented more similarities to each other than to group C. Conclusion: Acute injury of tendon treated with human amniotic membrane fragment showed a faster healing process, reduction in inflammatory response, intense proliferation of fibroblasts and organization of collagen fibers.

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Section: Barboni Et Alsupporting

confidence: 90%

Section: ■ Introductionmentioning

confidence: 99%

Amniotic membrane as an option for treatment of acute Achilles tendon injury in rats

et al. 2017

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“…However, independently of the therapeutic use of amniotic-derived cells, accurate long term preclinical/clinical studies to evaluate AEC safety and stability in host tissues are still required. In this context, one future target of our group will be to compare the allogeneic stability and regenerative properties of undifferentiated vs. differentiated AEC when injected into an experimental induced tendon defect [57], [58] performed on a medium sized mammal, the sheep, selected for its high translational value. Before the allotransplantation of the differentiated AEC into immunocompetent animals, their immunogenicity has to be tested trying to adapt the methods previously described in other animal models [52], [54]–[56].…”

Section: Discussionmentioning

confidence: 99%

Indirect Co-Culture with Tendons or Tenocytes Can Program Amniotic Epithelial Cells towards Stepwise Tenogenic Differentiation

et al. 2012

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BackgroundAmniotic epithelial cells (AEC) have potential applications in cell-based therapy. Thus far their ability to differentiate into tenocytes has not been investigated although a cell source providing a large supply of tenocytes remains a priority target of regenerative medicine in order to respond to the poor self-repair capability of adult tendons. Starting from this premise, the present research has been designed firstly to verify whether the co-culture with adult primary tenocytes could be exploited in order to induce tenogenic differentiation in AEC, as previously demonstrated in mesenchymal stem cells. Since the co-culture systems inducing cell differentiation takes advantage of specific soluble paracrine factors released by tenocytes, the research has been then addressed to study whether the co-culture could be improved by making use of the different cell populations present within tendon explants or of the high regenerative properties of fetal derived cell/tissue.Methodology/Principal FindingsFreshly isolated AEC, obtained from ovine fetuses at mid-gestation, were co-incubated with explanted tendons or primary tenocytes obtained from fetal or adult calcaneal tendons. The morphological and functional analysis indicated that AEC possessed tenogenic differentiation potential. However, only AEC exposed to fetal-derived cell/tissues developed in vitro tendon-like three dimensional structures with an expression profile of matrix (COL1 and THSB4) and mesenchymal/tendon related genes (TNM, OCN and SCXB) similar to that recorded in native ovine tendons. The tendon-like structures displayed high levels of organization as documented by the cell morphology, the newly deposited matrix enriched in COL1 and widespread expression of gap junction proteins (Connexin 32 and 43).Conclusions/SignificanceThe co-culture system improves its efficiency in promoting AEC differentiation by exploiting the inductive tenogenic soluble factors released by fetal tendon cells or explants. The co-cultural system can be proposed as a low cost and easy technique to engineer tendon for biological study and cell therapy approach.

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“…Before performing sinus augmentation, oAEC were marked with a fluorescence lipophilic dye, the PKH26, as previously described [44], [47], [62].…”

Section: Methodsmentioning

confidence: 99%

Synthetic Bone Substitute Engineered with Amniotic Epithelial Cells Enhances Bone Regeneration after Maxillary Sinus Augmentation

et al. 2013

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BackgroundEvidence has been provided that a cell-based therapy combined with the use of bioactive materials may significantly improve bone regeneration prior to dental implant, although the identification of an ideal source of progenitor/stem cells remains to be determined.AimIn the present research, the bone regenerative property of an emerging source of progenitor cells, the amniotic epithelial cells (AEC), loaded on a calcium-phosphate synthetic bone substitute, made by direct rapid prototyping (rPT) technique, was evaluated in an animal study.Material And MethodsTwo blocks of synthetic bone substitute (∼0.14 cm3), alone or engineered with 1×106 ovine AEC (oAEC), were grafted bilaterally into maxillary sinuses of six adult sheep, an animal model chosen for its high translational value in dentistry. The sheep were then randomly divided into two groups and sacrificed at 45 and 90 days post implantation (p.i.). Tissue regeneration was evaluated in the sinus explants by micro-computer tomography (micro-CT), morphological, morphometric and biochemical analyses.Results And ConclusionsThe obtained data suggest that scaffold integration and bone deposition are positively influenced by allotransplantated oAEC. Sinus explants derived from sheep grafted with oAEC engineered scaffolds displayed a reduced fibrotic reaction, a limited inflammatory response and an accelerated process of angiogenesis. In addition, the presence of oAEC significantly stimulated osteogenesis either by enhancing bone deposition or making more extent the foci of bone nucleation. Besides the modulatory role played by oAEC in the crucial events successfully guiding tissue regeneration (angiogenesis, vascular endothelial growth factor expression and inflammation), data provided herein show that oAEC were also able to directly participate in the process of bone deposition, as suggested by the presence of oAEC entrapped within the newly deposited osteoid matrix and by their ability to switch-on the expression of a specific bone-related protein (osteocalcin, OCN) when transplanted into host tissues.

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Experimental study on allografts of amniotic epithelial cells in calcaneal tendon lesions of sheep

Cited by 23 publications

References 5 publications

Amniotic membrane as an option for treatment of acute Achilles tendon injury in rats

Amniotic membrane as an option for treatment of acute Achilles tendon injury in rats

Indirect Co-Culture with Tendons or Tenocytes Can Program Amniotic Epithelial Cells towards Stepwise Tenogenic Differentiation

Synthetic Bone Substitute Engineered with Amniotic Epithelial Cells Enhances Bone Regeneration after Maxillary Sinus Augmentation

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