Experimental study on histopathological changes and tissue tropism of Iranian infectious bronchitis serotype 793/B-like virus in SPF chickens

Bijanzad, Peyman; Momayez, R; Bozorgmehri-Fard, M. H.; Hablolvarid, M.H.; Pourbakhsh, Seyed Ali

doi:10.4102/jsava.v84i1.970

Cited by 10 publications

(11 citation statements)

References 29 publications

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“…The bursa showed depletion of lymphocytes and necrosis but the bursal and thymus atrophy were associated with IBVs infection [32,33]. Proventriculus and intestine showed hyperplasia and degenerative changes in their epithelium.…”

Section: Discussionmentioning

confidence: 99%

Pathological Studies on Infectious Bronchitis Disease in Chickens

El-Fetouh

Mohamed

Refat

et al. 2016

Zagazig Veterinary Journal

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Infectious bronchitis virus has been recognized as highly contagious disease in chickens. In this study, 138 birds from 10 broiler chicken flocks that suffered from respiratory disease were examined to study the pathological lesions associated with IBV infection in naturally infected flocks. Moreover, the infection was confirmed via virus isolation and PCR. Samples were harvested from both Sharkia and Gharbia provinces during one year (October 2014-2015). The clinical examination of the investigated birds showed nasal discharges, gasping and rales. Congested trachea with lumen contained mucus and caseated material at the biforcation of the two main bronchi were characteristic in most of the necropsied birds. The kidneys were enlarged and dark red or pale with the presence of whitish precipitate in the ureters. Four isolates recovered from four different broilers chicken's flocks were further analyzed by S1 glycoprotein gene sequencing. The phylogenetic analysis of the deduced amino acids sequences of the four strains revealed that they were closely related to the IBV Egyptian variant 2 and IS/1494/2006 strains. The histopathological examination of the kidneys showed focal aggregation of lymphocytes in the renal cortex and the medulla. The liver showed multiple focal areas of necrosis which replaced by lymphocyte and heterophils aggregation. The spleen showed thickened capsule with subcapsular hemorrhage and mild to severe focal depletion of lymphocytes. Trachea, lungs, proventriculus, intestine and bursa showed hyperplasia and degenerative changes in their epithelium. Blood vessels showed endotheliosis with perivascular edema and leukocyte infiltration in various organs. Edema was also seen in heart, trachea and lungs. Finally, it could be concluded that infectious bronchitis virus is a major cause and strongly implicated in respiratory and/or renal problems of the Egyptian broiler chicken flocks. Moreover, Egyptian variant 2 still evolving and causing severe damage to renal tissue and various organs.

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Section: Discussionmentioning

confidence: 99%

Pathological Studies on Infectious Bronchitis Disease in Chickens

El-Fetouh

Mohamed

Refat

et al. 2016

Zagazig Veterinary Journal

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“…The first record of the 793/B serotype in Asia was in China in 2003; the virus isolate was named Taian-03, and the sequence of the S1 gene has been deposited in the GenBank database with the accession number AY837465. Currently, 793/B type viruses have been detected in several other Asian countries, including Japan (Mase et al, 2008), India (Sumi et al, 2012) and Iran (Bijanzed et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Serotype shift of a 793/B genotype infectious bronchitis coronavirus by natural recombination

Zhang

Han

et al. 2015

Infection, Genetics and Evolution

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An infectious bronchitis coronavirus, designated as ck/CH/LHLJ/140906, was isolated from an infectious bronchitis virus (IBV) strain H120-vaccinated chicken flock, which presented with a suspected infectious bronchitis virus (IBV) infection. A phylogenetic analysis based on the S1 gene clustered ck/CH/LHLJ/140906 with the 793/B group; however, a pairwise comparison showed that the 5' terminal of the S1 gene (containing hypervariable regions I and II) had high sequence identity with the H120 strain, while the 3' terminal sequence was very similar to that of IBV 4/91 strain. A SimPlot analysis of the complete genomic sequence, which was confirmed by a phylogenetic analysis and nucleotide similarities using the corresponding gene fragments, suggested that isolate ck/CH/LHLJ/140906 emerged from multiple recombination events between parental IBV strains 4/91 and H120. Although the isolate ck/CH/LHLJ/140906 had slightly higher S1 amino acid sequence identity to strain 4/91 (88.2%) than to strain H120 (86%), the serotype of the virus was more closely related to that of the H120 strain (32% antigenic relatedness) than to the 4/91 strain (15% antigenic relatedness). Whereas, vaccination of specific pathogen-free chickens with the 4/91 vaccine provided better protection against challenge with ck/CH/LHLJ/140906 than did vaccination with the H120 strain according to the result of virus re-isolation. As the spike protein, especially in the hypervariable regions of the S1 domain, of IBVs contains viral neutralizing epitopes, the results of this study showed that recombination of the S1 domain resulted in the emergence of a new serotype.

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“…In Iran, several serotypes and genotypes of IBV have been reported from different regions; the first isolation of IBV in Iranian chicken flocks was reported in 1994. Later, several Iranian researchers identified the 793/B serotype, which turned out to be one of the most predominant types of IBV circulating in the country (6). Genotyping of IBV strains isolated in Iran were classified into seven distinct phylogenetic groups (Mass, /B like, IS/1494 like, IS/720-like, QX-like, IR-1, and IR-2) based on analysis of mainly HVRs of the S1 glycoprotein gene (7,8).…”

Section: Introductionmentioning

confidence: 99%

Genotyping of Infectious Bronchitis Viruses in the East of Iran, 2015

et al. 2015

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Background and Aims: Infectious bronchitis virus (IBV) is the causative agent of avian infectious bronchitis, which is characterized by respiratory, reproductive, and renal signs. IBV is a highly variable virus with a large number of genotypes. S1 gene sequencing has been used for molecular epidemiological studies and genotypic characterization of IBV. To better understand the molecular epidemiology of IBV in Iran, we sequenced the S1 gene of IBV field isolates, a total of 40 tracheal and kidney tissue specimens from different commercial broiler flocks in the East of Iran were collected from 2015. Materials and Methods: 15 IBV-positive samples were selected from among the total and were further characterized by sequencing the spike glycoprotein gene. The isolates were confirmed by real-time polymerase chain reaction (PCR) and characterized by sequencing the spike glycoprotein gene. Results: Three genotypes were detected. The percentage of Variant 2 (IS/1494 like), 793/B, and QX genotypes was 66.7%, 26.7%, and 6.6% respectively. We reported the QX as the first time and Variant2 was the dominant genotype in this area. Conclusion: It is an updated and comprehensive study of genotyping of IBV and completes IBV puzzle in the East of Iran.

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Experimental study on histopathological changes and tissue tropism of Iranian infectious bronchitis serotype 793/B-like virus in SPF chickens

Cited by 10 publications

References 29 publications

Pathological Studies on Infectious Bronchitis Disease in Chickens

Pathological Studies on Infectious Bronchitis Disease in Chickens

Serotype shift of a 793/B genotype infectious bronchitis coronavirus by natural recombination

Genotyping of Infectious Bronchitis Viruses in the East of Iran, 2015

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