Experimental substantiation of «green» laser light application for selective photodestruction of capillary angiodysplasia of the skin

Abstract

Rationale. The existing techniques for treating skin capillary angiodysplasias, including «laser» photodestruction, are not effective enough and often cause undesirable thermal damage and cicatricial deformities of the skin. Thus, the effective removal of this vascular pathology is still relevant and requires further solution.Purpose. To study the nature of thermal injuries and wound regenerative processes after «green» laser irradiation with wavelength 525 nm so as to find out if it may be used for selective photodestruction of subepithelial pathological vascular structures, including angiodysplasias of skin capillaries.Material and Methods. Scallops of alive white chickens were taken as a biological model of vascular structures for in vivo studies. An experimental solid-state laser generating «green» light with wavelength 525 nm was used. This laser light has similar peaks of the absorption coefficient of hemoglobin and oxyhemoglobin which are found in skin capillaries. Features of damage and dynamics of regenerative process in the irradiated area were studied at the model of vascular structures using findings of morphological macroscopic and histological examinations.Results. Findings of the present experimental trial have inspired that selective photodestruction of subepithelial vascular structures of scallops is possible with minimal damage to the epithelium, including growth zones. In irradiated areas, one could see whitening and smoothing of the surface due to closure of vessel lumens in the subepithelial zone and formation of collagenosis layer there, as well as epithelialization of wound surface in physiological terms without any formation of cicatricial deformation of the skin.Conclusion. The selective effect of 525 nm «green» laser light at subepithelial vascular structures with minimal skin damage has been confirmed. It opens a way to apply this light for selective photodestruction of capillary angiodysplasias of the skin with good clinical and esthetic outcomes.