Experimental techniques for single cell and single molecule biomechanics

Lim, Chwee Teck; Zhou, Enhua H.; Li, A.; Vedula, Sri Ram Krishna; Fu, Hongxia

doi:10.1016/j.msec.2005.08.022

Cited by 132 publications

(89 citation statements)

References 55 publications

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“…[1][2][3][4] In recent years, a number of research groups found optical micromanipulation particularly useful for biological objects because of the ability of optical tweezers to precisely control the trapped object's position, orientation and speed [5][6][7][8]. Studies have been carried out on a wide range of cells from bacteria less than a micron in size, to mammalian cells with tens of microns in diameter (red blood cells and T cells) [9][10][11][12]. Micromanipulation via optical trapping forces involves direct focusing a laser beam on cell samples [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Optical micromanipulation

2008

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Section: Introductionmentioning

confidence: 99%

Optical micromanipulation

2008

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“…The study of RBCs at the cellular level has shown that the infection of RBCs can impair their rheological properties [11][12][13][14][15][16][17][18][19][20].…”

Section: Correlation With Diseasesmentioning

confidence: 99%

“…1.1(a)). Both elastic and viscoelastic properties of cells can be measured using this technique [3,10,11,25].…”

Section: Characterization Techniquesmentioning

confidence: 99%

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Automated Micropipette Aspiration of Single Cells

2013

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This research presents a system for mechanically characterizing single cells using automated micropipette aspiration. Using vision-based control and position control, the system controls a micromanipulator, a motorized translation stage, and a custom-built pressure system to position a micropipette (4 µm opening) to approach a cell, form a seal, and aspirate the cell into the micropipette for quantifying the cell's elastic and viscoelastic parameters as well as viscosity. Image processing algorithms were developed to provide controllers with real-time visual feedback and to accurately measure cell deformation behavior on the fly. Experiments on both solid-like and liquid-like cells demonstrated that the system is capable of efficiently performing single-cell micropipette aspiration and has low operator skill requirements. Once the system was validated, it was used to study voided urine cells. In this study, the mechanical properties of bladder carcinoma cells were investigated.ii

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