Experimental transmission of Hepatozoon americanum Vincent-Johnson et al., 1997 to dogs by the Gulf Coast tick, Amblyomma maculatum Koch

Mathew, John; Ewing, S. A.; Panciera, Roger J.; Woods, J. Paul

doi:10.1016/s0304-4017(98)00189-7

Cited by 86 publications

(55 citation statements)

References 12 publications

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“…17 The experimentally infected dogs were aged 6-9 months at exposure and were infected by ingestion of experimentally infected ticks or sporulated oocysts harvested from such ticks as previously described. 13 The coyote pups were approximately 10 weeks old at oral exposure to sporulated oocysts. 10 Experimentally infected dogs and coyotes were monitored daily.…”

Section: Methodsmentioning

confidence: 99%

“…Observations reported here were obtained by examination of four dogs and two coyote pups experimentally infected in the course of transmission studies 10,13 and eight naturally infected dogs that were included in a study of the tissue stages of H. americanum. 17 The experimentally infected dogs were aged 6-9 months at exposure and were infected by ingestion of experimentally infected ticks or sporulated oocysts harvested from such ticks as previously described.…”

Section: Methodsmentioning

confidence: 99%

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Skeletal Lesions of Canine Hepatozoonosis Caused by Hepatozoon americanum

et al. 2000

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Abstract. Canine hepatozoonosis, caused by Hepatozoon americanum, is an emerging tick-borne disease of dogs in North America. In addition to the skeletal and cardiac myositis that are prominent features of the disease, there is disseminated periosteal bone proliferation in most dogs that manifest clinical disease. Each of six experimentally infected animals (four dogs and two coyotes) and seven of eight naturally infected dogs had gross or histopathologic osteoproliferative lesions. Experimental animals were 6-9 months of age when exposed. Naturally infected dogs were 8 months to 11 years old when subjected to necropsy. Lesions occurred primarily on the diaphysis of the more proximal long bones of the limbs; however, flat and irregular bones were frequently involved. Lesions involving metacarpals, metatarsals, and digits were infrequent. The earliest observed periosteal lesions were in an experimentally infected dog 32 days after exposure to sporulated oocysts of H. americanum. There were hypertrophy and hyperplasia of osteoprogenitor cells, and osteoblasts appeared in the cellular zone of the periosteum. Spicules of woven bone oriented perpendicularly to bone cortex followed. Later yet, periosteal new bone was remodeled and tended to become oriented parallel to the cortical bone. Horizontally oriented zones of remodeled, condensed bone sometimes occurred in multiple layers on the original cortex, forming ''pseudocortices.'' The osseous lesions of American canine hepatozoonosis, with few variations, are remarkably similar to those of hypertrophic osteopathy in domestic dogs and other mammalian species, including humans.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Skeletal Lesions of Canine Hepatozoonosis Caused by Hepatozoon americanum

et al. 2000

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“…It is a vector of Rickettsia parkeri (Parker et al 1939;Sumner et al 2007), an agent that may cause disease in humans . It has also been demonstrated as an intermediate host for Hepatozoon americanum Vincent-Johnson et al (Eucoccidiorida: Hepatozoidae), the agent of American canine hepatozoonosis in dogs (Mathew et al 1998;Ewing et al 2002), and as an agent of tick paralysis in humans (Paffenbarger 1950).…”

Section: Adult Male: Bodymentioning

confidence: 99%

The Biological Survey of Canada: A Personal History

Danks¹

2017

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“…Antigen for the ELISA was prepared from sporozoites of H. americanum that developed in the invertebrate host, A. maculatum. Successful experimental infections of this tick and consistently high infection rates 11,12 of the ticks made development of this test possible. Sporozoites from experimentally infected ticks are an ideal source of antigen because they are the infective stages that are first exposed to the vertebrate host immune system.…”

Section: Discussionmentioning

confidence: 99%

“…3,16,18 The Gulf Coast tick (Amblyomma maculatum) is an excellent definitive host/vector for H. americanum. 12 Hepatozoon canis infection has long been diagnosed by demonstration of gamonts in circulating leukocytes. More recently, an indirect fluorescent antibody test has been developed that employs gamonts (recovered from buffy coat preparations) as antigen.…”

mentioning

confidence: 99%

An Indirect Enzyme-Linked Immunosorbent Assay for Diagnosis of American Canine Hepatozoonosis

et al. 2001

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Abstract. American canine hepatozoonosis (ACH), caused by Hepatozoon americanum, is an emerging tick-borne disease of dogs. An indirect enzyme-linked immunosorbent assay (ELISA) that should facilitate diagnosis of infection and study of the epidemiology of ACH has been developed using H. americanum sporozoites as antigen. Efficacy of the new test as a diagnostic tool was compared with that of skeletal muscle biopsy, the current gold standard for confirming H. americanum infection. Results show that the test is sensitive (93%) and specific (96%) and that it is as reliable as histopathologic examination of skeletal muscle for detecting infection. The ELISA would be suitable as a routine laboratory test for diagnosis of ACH.

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Experimental transmission of Hepatozoon americanum Vincent-Johnson et al., 1997 to dogs by the Gulf Coast tick, Amblyomma maculatum Koch

Cited by 86 publications

References 12 publications

Skeletal Lesions of Canine Hepatozoonosis Caused by Hepatozoon americanum

Skeletal Lesions of Canine Hepatozoonosis Caused by Hepatozoon americanum

The Biological Survey of Canada: A Personal History

An Indirect Enzyme-Linked Immunosorbent Assay for Diagnosis of American Canine Hepatozoonosis

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