Experimental Uraemia with Associated Plasma Amino Acid Abnormalities but without Retarded Food Intake and Weight Gain

Haines, D.J.; Swan, Caroline; Green, J.R.B.; Woodley, John

doi:10.1159/000185750

Cited by 4 publications

(7 citation statements)

References 6 publications

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“…Advanced uremia itself is associated with a reduction in dietary protein intake [98]. Azotemia per se is suggested to be responsible for the AA abnormalities since rats with mild CRF exhibit comparable AA abnormalities [99] compared to humans [67]. It has also been shown that in patients with CRF the metabolism of an oral protein load, both natural protein [100] as well as AA mixture [101], is impaired and that the flow of AAs to the organs is altered in the postprandial period.…”

Section: Malnutrition and Aas In Crfmentioning

confidence: 99%

Muscle Metabolism in Uremia and the Effect of Amino Acid Supplementation

Allein¹,

Verbeelen

1998

Nephron

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Section: Malnutrition and Aas In Crfmentioning

confidence: 99%

Muscle Metabolism in Uremia and the Effect of Amino Acid Supplementation

Allein¹,

Verbeelen

1998

Nephron

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“…Male Sprague-Dawley rats, weighing approximately 300g (range 273-347 g), were obtained from the University of Nottingham and were used for all experiments. Uraemia was induced by a two-stage surgical procedure as previously described [22]. Food with a protein content of 22.7% (w/w) and tap water were available ad libitum throughout the protocol.…”

Section: Animalsmentioning

confidence: 99%

“…The plasma creatinine concentration was determined in all blood samples by a modified Jaff6 reaction which has been described previously [22]. In addition, the plasma urea concentration was measured pre-operatively and at the time of killing by using a Jeol JLC 6AH amino acid analyser.…”

Section: Determination Of Plasma Creatine and Ureamentioning

confidence: 99%

“…The uraemic group showed a transient fall in body weight after each surgical step, but comparison of weight gain and food intake throughout the protocol showed no significant difference between the uraemic and shamoperated groups. These data are presented in detail elsewhere [22].…”

Section: Weight Gain and Food Intakementioning

confidence: 99%

“…In this study we have measured the activities of several peptide hydrolases and three non-peptidase brush-border marker enzymes in crude homogenates prepared from proximal, mid and distal small-intestinal mucosa obtained from uraemic and sham-operated rats. In the rat model of [ 17-2 11. chronic renal failure used, the level and duration of the induced uraemia has been well defined and the food intake of the uraemic and sham-operated animals was comparable [22].…”

Section: Introductionmentioning

confidence: 99%

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Mucosal Peptide Hydrolase and Brush-Border Marker Enzyme Activities in Three Regions of the Small Intestine of Rats with Experimental Uraemia

et al. 1990

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1. The activities of nine peptide hydrolases and three non-peptidase brush-border marker enzymes have been quantified in crude homogenates prepared from the proximal, mild and distal regions of small-intestinal mucosa for sham-operated (n = 9) and uraemic (n = 14) rats. Abnormalities in enzyme activities were observed in all regions studied in the uraemic group, although no reduction in food intake occurred. 2. The proximal region of the small intestine from uraemic rats showed a general fall in enzyme activities associated with the brush-border. This fall was combined with a decline in mucosal protein content. In contrast, the mid and distal regions showed increased activity against the dipeptide tyrosyl-glycine. 3. It is proposed that the fall in brush-border enzyme activities in the proximal small intestine of uraemic rats is a response to the increased water intake associated with this, and presumably other, rat models of uraemia. The increased enzyme activity against tyrosyl-glycine found in the mid and distal regions of the small intestine of uraemic rats may be caused by an increased small-intestinal transit rate, but could be an attempt to maximize tyrosine absorption in response to decreased plasma tyrosine levels. 4. This study casts doubt on specific activities being the most useful units of enzyme activity, when measured in crude homogenates prepared from the proximal small intestine of uraemic rats. It also demonstrates that enzyme activities measured at a single site in the small intestine of uraemic rats may not be representative of the enzymatic changes occurring in the small-intestinal mucosa as a whole.

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Plasma amino acids are highly dependent on acid-base balance in uremic rats

Gretz

Langer²,

Strauch

1992

Amino Acids

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Acidosis is a common finding in uremia. We analysed the effect of changes in acid-base balance in 42 Sprague-Dawley rats having longstanding uremia (> 3 mon). The rats were treated with CaCO3 or CaCl2 in a random cross-over trial after being divided into two dietary groups (8% or 18% protein). At the end of each observation period parameters were assessed. The main finding was that plasma amino and keto acid concentrations varied significantly with the acid-base balance and the protein intake. Furthermore a considerable degree of catabolism and anorexia was observed. In addition other confounding variables were observed, e.g. increased corticosterone excretion, electrolyte losses, and proteinuria. Our data underline that plasma amino acid concentrations in uremic rats are highly dependent on acid-base balance and overall protein intake.

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Experimental Uraemia with Associated Plasma Amino Acid Abnormalities but without Retarded Food Intake and Weight Gain

Cited by 4 publications

References 6 publications

Muscle Metabolism in Uremia and the Effect of Amino Acid Supplementation

Muscle Metabolism in Uremia and the Effect of Amino Acid Supplementation

Mucosal Peptide Hydrolase and Brush-Border Marker Enzyme Activities in Three Regions of the Small Intestine of Rats with Experimental Uraemia

Plasma amino acids are highly dependent on acid-base balance in uremic rats

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