Experimental validation of a Tokamak neutron spectrometer based on Bonner spheres

Cao, Jing; Jiang, C. H; Cao, Hongchao; Yin, Zhen‐Qiang

doi:10.1088/1674-1137/39/8/086001

Cited by 11 publications

(17 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[69][70][71] In this reaction cascade, GOx catalyzes the oxidation of glucose to produce H 2 O 2 , which is then used by HRP to oxidize 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS Red , colorless) into ABTS Ox (green). Experimentally, we first electrodeposited a chitosan film, peeled it from the electrode, 72,73 and sequentially contacted this film with solutions of ConA, glycogen, and finally, GOx and HRP (5 mg/mL). (Note that the sequence used to assemble GOx and HRP did not affect the results.)…”

Section: Assembling Biological Function To Multilayer Filmmentioning

confidence: 99%

Coupling Self-Assembly Mechanisms to Fabricate Molecularly and Electrically Responsive Films

Maniar

et al. 2019

Biomacromolecules

View full text Add to dashboard Cite

Biomacromolecules often possess information to self-assemble through low energy competing interactions which can make self-assembly responsive to environmental cues and can also confer dynamic properties. Here, we coupled self-assembling systems to create biofunctional multilayer films that can be cued to disassemble through either molecular or electrical signals. To create functional multilayers, we: (i) electrodeposited the pH-responsive self-assembling aminopolysaccharide chitosan, (ii) allowed the lectin Concanavalin A (ConA) to bind to the chitosan-coated electrode (presumably through electrostatic interactions), (iii) performed layer-bylayer self-assembly by sequential contacting with glycogen and ConA, and (iv) conferred biological (i.e., enzymatic) function by assembling glycoprotein (i.e., enzymes) to the ConAterminated multilayer. Because the ConA tetramer dissociates at low pH, this multilayer can be triggered to disassemble by acidification. We demonstrate two approaches to induce acidification: (i) glucose oxidase can induce multilayer disassembly in response to molecular cues, and (ii) anodic reactions can induce multilayer disassembly in response to electrical cues.

show abstract

Section: Assembling Biological Function To Multilayer Filmmentioning

confidence: 99%

Coupling Self-Assembly Mechanisms to Fabricate Molecularly and Electrically Responsive Films

Maniar

et al. 2019

Biomacromolecules

View full text Add to dashboard Cite

show abstract

“…38,55,56 The outer membrane protein that imports ferrichrome, FhuA, 57 was engineered by the Movileanu group to form a robust beta-barrel nanopore. 58 Another toxin-aerolysin 59 -was recently used to differentiate between different lengths of DNA homopolymers 22 and arginine homopeptides. 60 The four remaining biological nanopores are the viral portal protein G20C modified to insert into lipid bilayer membranes, 61 the alpha pore forming toxin FraC 62 used extensively for peptide detection, 63 a truncated version of the outer membrane porin MspA 64 used for DNA sequencing 11 and single molecule mea-surements, 65 and another alpha pore forming toxin ClyA 66 used, among others, 67 for single molecule enzymology.…”

Section: Validation: Ion Conductance Of Biological Nanoporesmentioning

confidence: 99%

“…To date, the nanopore sensing principle has been applied to study variety of biomolecular systems, 7,8 from ions 9 to viruses, 10 including determination of the nucleotide sequence of DNA 11 and RNA. 12 Tremendous opportunities lie ahead for nanopore sensing in protein character-ization [13][14][15] and sequencing technologies, 16,17 detection of molecular biomarkers, 18,19 drug design, 20 mass spectrometry 21,22 and general purpose analytics. 23,24 Realizing these goals will require development of custom nanopores that produce well-defined current modulations in the presence of target analytes.…”

mentioning

confidence: 99%

Rapid and Accurate Determination of Nanopore Ionic Current Using a Steric Exclusion Model

Wilson

Sarthak

et al. 2019

ACS Sens.

View full text Add to dashboard Cite

Nanopore sensing has emerged as a versatile approach to detection and identification of biomolecules. Presently, researchers rely on experience and intuition for choosing or modifying the nanopores to detect a target analyte. The field would greatly bene t from a computational method that could relate the atomic-scale geometry of the nanopores and analytes to the blockade nanopore currents they produce. Existing computational methods are either computationally too expensive to be used routinely in experimental laboratories or not sensitive enough to account for the atomic structure of the pore and the analytes. Here, we demonstrate a robust and inexpensive computational approach-the steric exclusion model (SEM) of nanopore conductance-that is orders of magnitude more efficient than all-atom MD and yet is sensitive enough to account for the atomic structure of the nanopore and the analyte. The method combines the computational efficiency of a finite element solver with the atomic precision of a nanopore conductance map to yield unprecedented speed and accuracy of ionic current prediction. We validate our SEM approach through comparison with the current blockades computed using the all-atom molecular dynamics method for a range of proteins confined to a solid-state nanopore, biological channels embedded in a lipid bilayer membranes and blockade currents produced by DNA homopolymers in MspA. We illustrate potential applications of SEM by computing blockade currents produced by nucleosome proteins in a solid-state nanopore, individual amino acids in MspA and by testing the effect of point mutations on amino acid distinguishability. We expect our SEM approach to become an integral part of future development of the nanopore sensing field. * These authors contributed equally to the manuscript Supplementary Information Available The following files are available free of charge: SI Wilson2019.pdf. Simulated dependence of KCl, K + and Cl − conductivity from the center of carbon, hydrogen, nitrogen, oxygen atoms; comparison of the nanopore currents computed using the resistor and SEM models; comparison of SEM and MD blockade currents in MspA done using nominal conductivity of KCl and for 3'-trans systems; supplementary methods detailing MD simulations of biological nanopores; and a table summarizing conditions for the biological nanopores simulations.

show abstract

“…Our group has produced several examples of early transition metal-catalyzed oxidative aminations of alkynes for the synthesis of pyrroles, [36][37][38][39][40] which proceeds through an electrocyclic reductive elimination to form a C-N bond from an azatitanacyclohexadiene intermediate ( Figure 1c, bottom right). 41 We envisioned that reaction of an analogous diazatitanacyclohexadiene 42 ' 43 -formed from the reaction between a nitrile and the common azatitanacyclobutene intermediate [44][45][46][47][48][49][50][51][52][53][54] -could result in a formal N-N bond reductive elimination to produce multisubstituted pyrazoles, either through catalytic turnover with azobenzene as an oxidant or by further reaction with an exogenous oxidant ( Figure 1c, bottom left). Herein, we report an example of oxidation-induced N-N bond reductive elimination, yielding pyrazoles from diazatitanacyclohexadienes (Figure 1c, top).…”

Section: Introductionmentioning

confidence: 99%

Multicomponent Pyrazole Synthesis from Alkynes, Nitriles, and Titanium Imido Complexes via Oxidatively Induced N–N Bond Coupling

et al. 2020

View full text Add to dashboard Cite

Pyrazoles are an important class of heterocycles found in a wide range of bioactive compounds and pharmaceuticals. Pyrazole synthesis often requires hydrazine or related reagents where an intact N-N bond is conservatively installed into a pyrazole precursor fragment. Herein, we report the multicomponent oxidative coupling of alkynes, nitriles, and Ti imido complexes for the synthesis of multisubstituted pyrazoles. This modular method avoids potentially hazardous reagents like hydrazine, instead forming the N-N bond in the final step via oxidation-induced coupling on Ti. The mechanism of this transformation has been studied in-depth through stoichiometric reactions of the key diazatitanacyclohexadiene intermediate, which can be accessed

show abstract

Experimental validation of a Tokamak neutron spectrometer based on Bonner spheres

Abstract: Experimental validation of a Tokamak neutron spectrometerbased on Bonner spheres * CAO Jing( ) 1,2;1) JIANG Chun-Yu( ) 1,2 CAO Hong-Rui( ) 1,2 YIN Ze-Jie( ) 1,2;2)

Cited by 11 publications

References 10 publications

Coupling Self-Assembly Mechanisms to Fabricate Molecularly and Electrically Responsive Films

Coupling Self-Assembly Mechanisms to Fabricate Molecularly and Electrically Responsive Films

Rapid and Accurate Determination of Nanopore Ionic Current Using a Steric Exclusion Model

Multicomponent Pyrazole Synthesis from Alkynes, Nitriles, and Titanium Imido Complexes via Oxidatively Induced N–N Bond Coupling

Contact Info

Product

Resources

About