Experimental validation of efficiency of probiotics in combined therapy of destructive pancreatitis

Abstract

The mechanism of E. coli translocation after its intragastral administration was studied in rats with acute pancreatitis. Bacterial dissemination into visceral organs was shown. Therapy with probiotic sporobacterin was more effective than antibiotic (cefaxon) therapy. Contamination of the viscera with Escherichia was notably decreased.

The Effects of Probiotic Supplementation on Experimental Acute Pancreatitis: A Systematic Review and Meta-Analysis

“…6b ). The results of the studies presenting continuous data (except for van Minnen et al [26] and Tarasenko et al [16] , after 2 h) are in accordance with the results of the meta-analysis ( Fig. 6c ).…”

“…Initially, 21 papers seemed to meet our selection criteria. After studying the full-text articles, 13 original studies remained [5] , [14] , [16] , [17] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] ( Fig. 1 ).…”

“…Because mortality was not intended to be a primary outcome measure in most of the animal studies, the experiments may have been underpowered to be able to detect a possible significant difference in mortality between probiotic treated and control groups. Even though Tarasenko et al 2000 [16] and Mangiante et al 2001 [5] did not provide data on mortality, the former emphasized that B. subtilis did not aggravate the course of experimental AP and the latter stressed that, as a proof of the safety of the probiotic prophylaxis, they never found L. plantarum in blood samples, also not in samples from animals with BT. In other words, although only very few animal experiments had been carried out and data on mortality available were not fully reliable, there were at the start of the PROPATRIA trial no indications from animal experiments that probiotics might have a harmful effect.…”

“…The protocol referred (indirectly) to one animal study published before the start of the trial (namely Mangiante et al 2001 [5] ), which showed some evidence for reduced bacterial translocation to the pancreas and MLN, but did not study the effect of probiotics during AP on mortality. We were able to identify three other animal studies that had already been published at the time (Deng et al 2000 [22] , Tarasenko et al 2000 [16] , Akyol et al 2003 [20] ). Of these three, only one study (Akyol et al 2003 [20] ) reported data on mortality and this study showed a non-significant reduction of mortality.…”

“…If continuous data were presented, data were discussed and presented in the tables but not included in meta-analysis. Where outcomes were measured repeatedly on different time points, we used the time point at which the measured efficacy was greatest [16] . In one study, histopathological data was presented as median and percentiles, these data were converted to mean and SD [17] .…”