Degradation of the electrowetting effect by a repeated actuation is evaluated over an extended period (200 min) on electrowetting-on-dielectric (EWOD) samples for three popular fluoropolymer top coatings: Teflon, FluoroPel and Cytop. A conductive liquid droplet is tested in an air environment at Electrowetting number Ew ≅ 0.34. A pulse train (6 sec period and 50% duty cycle) of three different voltage types is used for the actuation: positive DC, negative DC and 1 kHz AC. For the DC actuations, electrowetting degrades gradually on Cytop but significantly faster on Teflon and FluoroPel under the tested conditions. For the AC actuation, electrowetting degrades gradually on all three materials in a similar fashion.