In this paper, electrical characteristic analysis and corresponding experimental tests on gold bonding wire are presented. Firstly, according to EIA (Electronic Industries Association)/JEDEC97 standards, this paper establishes the electromagnetic structure model of gold bonding wire. The parameters, including flat length ratio, diameter, span and bonding height, were analyzed. In addition, the influence of three kinds of loops of bonding wire is discussed in relation to the S parameters. An equivalent circuit model of bonding wire is proposed. The effect of bonding wire on signal transmission was analyzed by eye diagram as well. Secondly, gold bonding wire design and measurement experiments were implemented based on radio frequency (RF) circuit theory analysis and test methods. Meanwhile, the original measurement data was compared with the simulation model data and the error was analyzed. At last, the data of five frequency points were processed to eliminate the fixture error as much as possible based on port embedding theory. The measurement results using port extension method were compared with the original measurement data and electromagnetic field simulation data, which proved the correctness of the simulation results and design rules.