Experimental Verification of a Sequence-Based Prediction: F
 1
 F
 0
 -Type ATPase of
 Vibrio cholerae
 Transports Protons, Not Na
 +
 Ions

Dzioba, Judith; Häse, Claudia C.; Gosink, Khoosheh K.; Galperin, Michael Y.; Dibrov, Pavel

doi:10.1128/jb.185.2.674-678.2003

Cited by 26 publications

(20 citation statements)

References 38 publications

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“…P. marinus SS120 contains several systems that are likely critical in the adaptation to the marine environment. Based on the sequence of the c subunit (AtpE) of its H ϩ -ATP synthetase (50), it appears that, in addition to (or instead of) H ϩ ions, this enzyme can also transport Na ϩ . Unlike many marine bacteria, P. marinus SS120 does not encode a primary Na ϩ pump.…”

Section: P Marinus Ss120 As a Minimal Genome Of An Oxyphototrophicmentioning

confidence: 99%

Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome

Dufresne

Salanoubat

Partensky

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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441

352

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Prochlorococcus marinus, the dominant photosynthetic organism in the ocean, is found in two main ecological forms: high-light-adapted genotypes in the upper part of the water column and low-lightadapted genotypes at the bottom of the illuminated layer. P. marinus SS120, the complete genome sequence reported here, is an extremely low-light-adapted form. The genome of P. marinus SS120 is composed of a single circular chromosome of 1,751,080 bp with an average G؉C content of 36.4%. It contains 1,884 predicted protein-coding genes with an average size of 825 bp, a single rRNA operon, and 40 tRNA genes. Together with the 1.66-Mbp genome of P. marinus MED4, the genome of P. marinus SS120 is one of the two smallest genomes of a photosynthetic organism known to date. It lacks many genes that are involved in photosynthesis, DNA repair, solute uptake, intermediary metabolism, motility, phototaxis, and other functions that are conserved among other cyanobacteria. Systems of signal transduction and environmental stress response show a particularly drastic reduction in the number of components, even taking into account the small size of the SS120 genome. In contrast, housekeeping genes, which encode enzymes of amino acid, nucleotide, cofactor, and cell wall biosynthesis, are all present. Because of its remarkable compactness, the genome of P. marinus SS120 might approximate the minimal gene complement of a photosynthetic organism.

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Section: P Marinus Ss120 As a Minimal Genome Of An Oxyphototrophicmentioning

confidence: 99%

Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome

Dufresne

Salanoubat

Partensky

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

441

352

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“…The emerging ion selectivity pattern in membrane PPases formally resembles that of ATP-powered cation pumps: ATPdependent H ϩ pumps are strictly H ϩ -specific (26,27), whereas Na ϩ pumps (28 -30) and Na ϩ ,K ϩ pumps (31) are capable of transporting H ϩ at low [Na ϩ ]. There is, however, one principal difference between the PP i -and ATP-driven transporters.…”

mentioning

confidence: 99%

Membrane Na+-pyrophosphatases Can Transport Protons at Low Sodium Concentrations

Luoto

Nordbo

Baykov

et al. 2013

Journal of Biological Chemistry

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“…The Na + -NQR is the major respiratory Na + pump in aerobic pathogens (Häse et al 2001;Reyes-Prieto et al 2014). Although not homologous to the respiratory Complex I (Dibrov et al 2004;Dzioba et al 2003;Reyes-Prieto et al 2014;Steuber et al 2014), it occupies the same position in the electron transport chain, using the oxidation of NADH by ubiquinone to drive translocation of Na + across the membrane. Reduction of quinone by the NQR complex is tightly coupled with Na + translocation (Juárez et al 2009;Steuber et al 2014) and is inhibited by the quinone analogs HQNO and korormicin (Hayashi et al 2002;Nakayama et al 1999;Yoshikawa et al 1997).…”

Section: Introductionmentioning

confidence: 99%

“…All chlamydial genomes encode a complete Na + cycle including the Na + -translocating NADH:ubiquinone oxidoreductase (Na + -NQR) as a major primary Na + pump (Dibrov et al 2004;Dzioba et al 2003;Häse et al 2001;Reyes-Prieto et al 2014). The Na + -NQR is the major respiratory Na + pump in aerobic pathogens (Häse et al 2001;Reyes-Prieto et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Development of a novel rationally designed antibiotic to inhibit a nontraditional bacterial target

Dibrov

Maddaford

et al. 2017

Can. J. Physiol. Pharmacol.

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Abstract:The search for new nontraditional targets is a high priority in antibiotic design today. Bacterial membrane energetics based on sodium ion circulation offers potential alternative targets. The present work identifies the Na + -translocating NADH: ubiquinone oxidoreductase (Na + -NQR), a key respiratory enzyme in many microbial pathogens, as indispensible for the Chlamydia trachomatis infectious process. Infection by Chlamydia trachomatis significantly increased first H + and then Na + levels within the host mammalian cell. A newly designed furanone Na + -NQR inhibitor, PEG-2S, blocked the changes in both H + and Na + levels induced by Chlamydia trachomatis infection. It also inhibited intracellular proliferation of Chlamydia trachomatis with a half-minimal inhibitory concentration in the submicromolar range but did not affect the viability of mammalian cells or bacterial species representing benign intestinal microflora. At low nanomolar concentrations (IC 50 value = 1.76 nmol/L), PEG-2S inhibited the Na + -NQR activity in sub-bacterial membrane vesicles isolated from Vibrio cholerae. Taken together, these results show, for the first time, that Na + -NQR is critical for the bacterial infectious process and is susceptible to a precisely targeted bactericidal compound in situ. The obtained data have immediate relevance for many different diseases caused by pathogenic bacteria that rely on Na + -NQR activity for growth, including sexually transmitted, pulmonary, oral, gum, and ocular infections.Key words: Na + -translocating NADH:ubiquinone oxidoreductase, Na + -NQR, antibiotic design, Chlamydia, bacteria, infection.Résumé : La recherche de nouvelles cibles non traditionnelles est aujourd'hui une grande priorité du domaine de la conception d'antibiotiques. Les propriétés énergétiques des membranes bactériennes fondées sur le transfert des ions sodiques offrent d'autres choix de cibles potentielles. Les présents travaux traitent de la NADH : ubiquinone oxydoréductase induisant la translocation des ions Na + (Na + -NQR), une enzyme clé de la respiration cellulaire chez beaucoup de microbes pathogènes, comme élément indispensable du processus infectieux associé à Chlamydia trachomatis. L'infection par Chlamydia trachomatis entraînait une augmentation marquée des taux d'ions H + puis d'ions Na + à l'intérieur des cellules de mammifère hôtes. Le PEG-2S, une furanone inhibitrice de la Na + -NQR nouvellement conçue, inhibait la variation des taux d'ions H + comme d'ions Na + induite par l'infection par Chlamydia trachomatis. Il inhibait aussi la prolifération intracellulaire de Chlamydia trachomatis avec une concentration minimale inhibitrice 50 de l'ordre du submicromolaire, mais il n'affectait pas la viabilité des cellules de mammifère ni d'espèces bactériennes représentant la flore microbienne intestinale bénigne. À des concentrations nanomolaires faibles (CI 50 = 1,76 nmol/L), le PEG-2S inhibait l'activité de la Na + -NQR dans les vésicules bactériennes submembrannaires isolées de Vibrio cholerae. Dans l'ense...

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Experimental Verification of a Sequence-Based Prediction: F ₁ F ₀ -Type ATPase of Vibrio cholerae Transports Protons, Not Na ⁺ Ions

Abstract: The membrane energetics of the intestinal pathogen

Cited by 26 publications

References 38 publications

Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome

Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome

Membrane Na+-pyrophosphatases Can Transport Protons at Low Sodium Concentrations

Development of a novel rationally designed antibiotic to inhibit a nontraditional bacterial target

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Experimental Verification of a Sequence-Based Prediction: F 1 F 0 -Type ATPase of Vibrio cholerae Transports Protons, Not Na + Ions

Abstract: The membrane energetics of the intestinal pathogen

Cited by 26 publications

References 38 publications

Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome

Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome

Membrane Na+-pyrophosphatases Can Transport Protons at Low Sodium Concentrations

Development of a novel rationally designed antibiotic to inhibit a nontraditional bacterial target

Contact Info

Product

Resources

About

Experimental Verification of a Sequence-Based Prediction: F ₁ F ₀ -Type ATPase of Vibrio cholerae Transports Protons, Not Na ⁺ Ions