ExperimentalEscherichia coli urinary infection in the rat

Taylor, Peter W.; Koutsaimanis, K. G.

doi:10.1038/ki.1975.106

Cited by 14 publications

(8 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Escherichia coli causes about three-quarters of all urinary tract infections (UTI), regardless of whether symptoms are evident, as in cystitis and pyelonephritis, or not evident, as in asymptomatic bacteriuria (20). Infections are assumed to be instigated via the ascending route by bacterial strains present in the host feces, but it now seems clear that such strains exhibit characteristics, for example, adherence to urinary tract epithelial cells and survival of complement action, which appear to contribute to urinary tract virulence and which are not as frequently observed among the normal fecal flora (2,12,14,18,23,25,29).…”

mentioning

confidence: 99%

Hemolysin Production as a Virulence Marker in Symptomatic and Asymptomatic Urinary Tract Infections Caused by Escherichia coli

Hughes¹,

Hacker²,

Roberts

et al. 1983

Infect Immun

112

View full text Add to dashboard Cite

Potential virulence, as defined by combined levels of adhesion to urinary epithelial cells, serum resistance, and mouse toxicity, was assessed for Escherichia coli strains causing symptomatic and asymptomatic urinary tract infections in relation to the carriage of hemolysin and other suspected virulence determinants. Hemolysin production (Hly), associated with certain 0 (04, 06, 018, and 075), K (5), and hemagglutination (VI and VII) antigenic types but not colicin V production (Cva), was evident in 83 and 60% of isolates in groups possessing high potential virulence and in only 11 and 6% of those with low virulence. Strains of particular 0-types were not more virulent per se, but among the serotypes, specific combinations of virulence factors appeared decisive, e.g., 018 HAVI B/D/G Hly+ K5'/and 018 HAIII/IVB/V Hly-Cva'/-K1'/strains were, respectively, of high and low potential virulence. Isolates with high potential virulence were found to a similar extent in symptomatic and asymptomatic infections. 546 on July 3, 2020 by guest http://iai.asm.org/ Downloaded from

show abstract

mentioning

confidence: 99%

Hemolysin Production as a Virulence Marker in Symptomatic and Asymptomatic Urinary Tract Infections Caused by Escherichia coli

Hughes¹,

Hacker²,

Roberts

et al. 1983

Infect Immun

112

View full text Add to dashboard Cite

show abstract

“…It was previously suggested (Taylor, 1975 ;Taylor and Koutsaimanis, 1975) that the rough characteristics of strain I7gb, and its failure to cause kidney infection in experimentally infected rats, might be due to the loss of a single protein factor from the cell surface. The results of gel electrophoresis of envelope proteins ( fig.…”

Section: Discussionmentioning

confidence: 99%

“…Both colonially rough forms spontaneously agglutinate in 0.2 % acriflavine. Whereas strains LP729 and I7 are able to cause kidney infection in an experimental rat model, strains I7fa and I7gb are unable to do so (Taylor and Koutsaimanis, 1975). These strains are described in detail by Taylor (1975).…”

mentioning

confidence: 99%

A Protein Factor Associated With Serum Resistance in Escherichia Coli

Taylor

Parton

1977

Journal of Medical Microbiology

View full text Add to dashboard Cite

IT has long been known that many strains of gram-negative bacteria are insensitive to the bactericidal action of normal human serum despite the presence in the serum of bactericidal antibody and complement (Georgevitch, 1926;Mackie and Finkelstein, 1931); the nature of this resistance remains obscure. Serum resistance in Escherichia coZi has been variously ascribed to the amount of cell-wall lipopolysaccharide (LPS), the length and number of LPS 0-side chains and to both the amount and haemagglutination-inhibiting activity of acidic polysaccharide K antigen (see review by Inoue, 1972). In a recent study (Taylor, 1975), it was found that none of these factors was able to explain the behaviour of serum-resistant mutants derived from smooth, E. coli strains isolated from the urinary tract, and it was suggested that these mutants synthesised an additional surface component, possibly protein in nature, which was responsible for serum resistance.The present study concerns the analysis of one of the serum-resistant mutants and the corresponding parent strain by gel-diffusion techniques and by sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis of cellenvelope proteins in an attempt to determine the basis of the mutation to serum resistance. Colonially rough mutants isolated from cultures of the serum-resistant mutant were also examined.MATERIALS AND METHODS BacteriaZ strains. E. coli no. LP729 is a smooth strain of serotype 0 9 which is rapidly killed by human serum after a delay of 1 h; E. coZi no. I7 is a serum-resistant mutant derived from strain LP729 by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine (Adelberg, Mandel and Chen, 1965). Both strains produce negligible amounts of K antigen, are stable in 0.2% acriflavine and synthesise LPS containing large amounts of mannose, a sugar confined to the 0-side chain of LPS from E. coli 0 9 strains. E. coli nos. I7fa and I7gb are colonially rough mutants plated from an aged (4-week) nutrient-broth culture of strain 17. E. coli no. I7fa is rapidly killed by serum and is a rfb mutant (Schmidt, Jann and Jann, 1969) with a complete LPS R2 core but defective in the synthesis of the LPS 09-side chain. Strain I7gb possesses a full complement of LPS 0-side chains and interacts with serum in an identical way to strain LP729. Both colonially rough forms spontaneously agglutinate in 0.2 % acriflavine. Whereas strains LP729 and I7 are able to cause kidney infection in an experimental rat model, strains I7fa and I7gb are unable to do so (Taylor and Koutsaimanis, 1975). These strains are described in detail by Taylor (1975).

show abstract

“…cell LP1674 was found to depend on the phase of growth of the cells prior to their addition to serum; this strain produces an extracellular polysaccharide containingN-acetyl-neuraminic acid [17]. E. coil LP729, which produces no exopolysaccharide, is rapidly killed by serum after a delay of 1 h [16,21]; E. coli LP1395 is capable of rapid growth in serum and produces a hexuronic acidcontaining exopolysaccharide [17]. The strains were grown in the nutrient-limited media of Evans et al [22] in a 1.liter chemostat (L.H.…”

Section: Organisms and Growth Conditionsmentioning

confidence: 99%

The effect of the growth environment on the serum sensitivity of some urinary Escherichia coli strains

Taylor

1978

FEMS Microbiology Letters

View full text Add to dashboard Cite

ExperimentalEscherichia coli urinary infection in the rat

Cited by 14 publications

References 13 publications

Hemolysin Production as a Virulence Marker in Symptomatic and Asymptomatic Urinary Tract Infections Caused by Escherichia coli

Hemolysin Production as a Virulence Marker in Symptomatic and Asymptomatic Urinary Tract Infections Caused by Escherichia coli

A Protein Factor Associated With Serum Resistance in Escherichia Coli

The effect of the growth environment on the serum sensitivity of some urinary Escherichia coli strains

Contact Info

Product

Resources

About