In Expt 1 40 lactating British Friesians, 20 cows and 20 heifers, were used to study the effect of crude protein (CP) content of the whole ration on milk yield, milk composition and live-weight change when maize silage was fed as the basal ration. There were four treatments with five cows and five heifers on each. During lactation weeks 4-12 the cows on treatments 1, 2, 3 and 4 all received 7 kg maize silage dry matter (D.M.) plus 8 kg D.M./day of a concentrate containing either 14, 18, 22 or 24% CP, respectively; heifers received 1 kg/day less of both silage and concentrate. This produced whole-ration CP contents of 11-7, 13-9, 16-0 and 17-1%. In the subsequent lactation weeks 13-20 silage feeding was increased to 9 kg D.M./cow/day and concentrate feeding decreased to 5 kg D.M./cow/day. Heifers again received 1 kg/day less of both silage and concentrate. This decreased whole-ration CP contents to 10-5, 11-7, 12-9 and 13-4%.During lactation weeks 4-12 and 13-20, with the exception of milk fat content in weeks 13-20, there were significant linear effects of whole-ration CP content on milk yield, milk fat, protein, lactose and total solids. There were no significant curvilinear relationships. Thus, despite the fact that the highest numerical values were generally recorded for the animals on treatment 3, the results indicate that a whole-ration CP content of at least 17-1 and 13-4% are required in early and mid-lactation respectively.In Expt 2 the loss of D.M., acid-detergent fibre and nitrogen from maize silage suspended in nylon bags in the rumen was measured. Compared with feeding either a low or high protein supplement, losses were greater for silage fed alone. If it is accepted that nitrogen loss can be approximated to protein degradability, then the value for maize silage was between 0-6 and 0-7. Using the same technique in Expt 3, comparable nitrogen losses for fish meal, decorticated groundnut meal and soya-bean meal were 0-3, 0-9 and 0-9, respectively, after 24 h incubation.