The human population living in the Nile basin countries is projected to double by 2050, approaching one billion 1 . The increase in water demand associated with this burgeoning population will put significant stress on the available water resources. Potential changes in the flow of the Nile River as a result of climate change may further strain this critical situation 2,3 . Here, we present empirical evidence from observations and consistent projections from climate model simulations suggesting that the standard deviation describing interannual variability of total Nile flow could increase by 50% (±35%) (multi-model ensemble mean ±1 standard deviation) in the twenty-first century compared to the twentieth century. We attribute the relatively large change in interannual variability of the Nile flow to projected increases in future occurrences of El Niño and La Niña events 4,5 and to observed teleconnection between the El Niño-Southern Oscillation and Nile River flow 6,7 . Adequacy of current water storage capacity and plans for additional storage capacity in the basin will need to be re-evaluated given the projected enhancement of interannual variability in the future flow of the Nile river.The Nile river basin is an ecosystem under severe stress. The basin is shared by about 400 million people in eleven countries with economies that depend heavily on agriculture, which employs the vast majority of the labour force in most of these countries 1 . Furthermore, almost half of the Nile basin countries are projected to live below the water scarcity level, 1,000 m 3 /person/year, by 2030 8,9 . Thus, any future changes in the magnitude of the flow volume of the Nile river can lead to significant impacts on the lives of people living within the basin and may increase the already high level of water stress.To fully utilize the water resources of the basin, several dams were built in the previous century to control the seasonal and interannual variability of the Nile flow. The recent conflict over the Nile water has received significant attention in the past few years after the decision by Ethiopia to build a large dam on the Blue Nile (the Grand Ethiopian Renaissance Dam, or GERD) to produce electricity, mostly for export to neighbouring countries. The dam, currently under construction, is relatively large compared to previous designs for the same location, which raised serious concerns regarding its effect on water shares of downstream countries (that is, Egypt and Sudan). If variability of the Nile flow changes in the future, then water storage capacity in the basin will need to be re-evaluated.Until recently, attempts to project the future of the Nile flow yielded inconsistent results. Although several studies examined the impacts of climate change on the Nile basin using different approaches 10-18 , the uncertainty surrounding conclusions from these studies was high for several reasons. First, none of the previous studies presented observational evidence to support their hypotheses, as they estimated the impacts of cli...