Exploitation of De Novo Helper-Lipids for Effective Gene Delivery.

Kurosaki, Tomoaki; Kitahara, Takashi; Teshima, Mugen; Nishida, Koyo; Nakamura, Junzo; Nakashima, Makoto; To, Hideto; Hukuchi, Hiromitsu; Hamamoto, Tomoyuki; Sasaki, Hidetada

doi:10.18433/j31s3b

Cited by 19 publications

(11 citation statements)

References 43 publications

(47 reference statements)

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“…Plasmid DNA-alkylphospholipid lipoplexes containing dioctadecyldimethylammonium bromide (DODAB) injected intratumorally significantly reduced tumor size compared with injection of naked DNA and Lipofectin-DNA complexes [67][68][69]. In addition to the classical approach of using monovalent quaternary ammonium compounds, such as DODAB and 1,2-dioleoyl-3-(trimethylammonium) propane (DOTAP), and cationic derivatives of cholesterol, new compounds such as reducible cationic lipids, disulfide-bond bearing lipids, acid-labile cationic lipids and glycine betaine derivatives have also been developed to overcome barriers to DNA delivery [70].…”

Section: ´53 3´nmentioning

confidence: 99%

Advancing Nonviral Gene Delivery: Lipid- and Surfactant-Based Nanoparticle Design Strategies

et al. 2010

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Gene therapy is a technique utilized to treat diseases caused by missing, defective or overexpressing genes. Although viral vectors transfect cells efficiently, risks associated with their use limit their clinical applications. Nonviral delivery systems are safer, easier to manufacture, more versatile and cost effective. However, their transfection efficiency lags behind that of viral vectors. Many groups have dedicated considerable effort to improve the efficiency of nonviral gene delivery systems and are investigating complexes composed of DNA and soft materials such as lipids, polymers, peptides, dendrimers and gemini surfactants. The bottom-up approach in the design of these nanoparticles combines components essential for high levels of transfection, biocompatibility and tissue-targeting ability. This article provides an overview of the strategies employed to improve in vitro and in vivo transfection, focusing on the use of cationic lipids and surfactants as building blocks for nonviral gene delivery systems.

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Section: ´53 3´nmentioning

confidence: 99%

Advancing Nonviral Gene Delivery: Lipid- and Surfactant-Based Nanoparticle Design Strategies

et al. 2010

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“…DOTMA-Chol (1: 1 molar ratio) and DOTMA-DOPE (1: 1 molar ratio) liposomes were prepared according to a previous report (18). Lipids were dissolved in chloroform.…”

Section: Preparation Of Cationic Liposomesmentioning

confidence: 99%

Chondroitin Sulfate Capsule System for Efficient and Secure Gene Delivery

Kurosaki

Kitahara

Fumoto³

et al. 2010

J Pharm Pharm Sci

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ABSTRUCT-Purpose. In this study, we developed various ternary complexes of encapsulated polyplexes and lipoplexes using chondroitin sulfate (CS) and investigated their universal usefulness for gene delivery. Methods. To prepare the cationic complexes, pDNA was mixed with some cationic vectors such as poly-L-arginine, poly-L-lysine, N-[1-(2, 3-dioleyloxy) propyl]-N, N, N-trimethylammonium chloride (DOTMA)-cholesterol liposomes, and DOTMA-dioleylphosphatidylethanolamine (DOPE) liposomes. CS was added to the cationic complexes for constructions of ternary complexes. We examined in vitro transfection efficiency, cytotoxicity, hematotoxicity, and in vivo transfection efficiency of the ternary complexes. Result. The cationic polymers and cationic liposomes bound to pDNA and formed stable cationic polyplexes and lipoplexes, respectively. Those cationic complexes showed high transgene efficiency in B16-F10 cells; however, they also had high cytotoxicity and strong agglutination with erythrocytes. CS could encapsulate the polyplexes and lipoplexes and form stable anionic particles without disrupting their structures. The ternary complexes encapsulated by CS showed high transgene efficiency in B16-F10 cells with low cytotoxicity and agglutination. As the result of animal experiments, the polyplexes had little transgene efficiency after intravenous administration in mice, whereas polyplexes encapsulated by CS showed specifically high transgene efficiency in the spleen. The capsulation of CS, however, reduced the high transgene efficiency of the lipoplexes. Conclusion. These results indicate that CS can contribute to polyplex-mediated gene delivery systems for effective and safe gene therapy. _______________________________________________________________________________________

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“…Lipoplexes have been used heavily as in vitro experimental tools but also have been successfully used for efficient systemic delivery of DNA vectors in vivo (Kurosaki et al, 2008). Targeted lipoplexes have been tested by conjugating antibodies (Ab), sugars, and small ligands for surface receptors.…”

Section: Machinery For Gene Deliverymentioning

confidence: 99%

Molecular-Genetic Imaging of Cancer

Minn¹,

Menezes²,

Sarkar³

et al. 2014

Advances in Cancer Research

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Molecular-genetic imaging of cancer using nonviral delivery systems has great potential for clinical application as a safe, efficient, noninvasive tool for visualization of various cellular processes including detection of cancer, and its attendant metastases. In recent years, significant effort has been expended in overcoming technical hurdles to enable clinical adoption of molecular-genetic imaging. This chapter will provide an introduction to the components of molecular-genetic imaging and recent advances on each component leading to safe, efficient clinical applications for detecting cancer. Combination with therapy, namely, generating molecular-genetic theranostic constructs, will provide further impetus for clinical translation of this promising technology.

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Exploitation of De Novo Helper-Lipids for Effective Gene Delivery.

Abstract: -Purpose. In this experiment, we developed new cationic liposomes as a vector, which form the complex with plasmid DNA

Cited by 19 publications

References 43 publications

Advancing Nonviral Gene Delivery: Lipid- and Surfactant-Based Nanoparticle Design Strategies

Advancing Nonviral Gene Delivery: Lipid- and Surfactant-Based Nanoparticle Design Strategies

Chondroitin Sulfate Capsule System for Efficient and Secure Gene Delivery

Molecular-Genetic Imaging of Cancer

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