Exploitation of genus Rhodosporidium for microbial lipid production

Xu, Jingyang; Liu, Dehua

doi:10.1007/s11274-017-2225-6

Cited by 53 publications

(34 citation statements)

References 69 publications

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“…This biomass has three main components – cellulose (40–53%), hemicellulose (11–36%), and lignin (15–28%) – and can be obtained from four different sources . Some of these lignocellulosic materials with high potential to be used as a feedstock are wood wastes derived from the forestry industry such as sawdust, chips, wood pyrolysates, and tree branches . However, there is also another type of waste that can be used as a carbon source to obtain fatty acids from Rhodosporidium .…”

Section: Feedstock Utilized By Rhodosporidium Sp To Produce Fatty Acmentioning

confidence: 99%

“…Only a few focus on other types of challenges such as the inhibitor management with respect to some of the substrates that are used for the production of lipids. Due to the limitation of substrate variation, it is necessary to adapt, modify, and develop strains with the aim of improving the utilization of different sugars in hydrolysates obtained from lignocellulosic biomass, as well increasing the tolerance to inhibitory compounds present in them . In this regard, synthetic biology and bioengineering are essential tools to improve the production and adaptation capacities that this strain presents .…”

Section: Challenges In Lipid Production In Rhodosporidium Genrementioning

confidence: 99%

“…Oleaginous yeast is highly adaptable to different carbon sources and has a high tolerance to inhibitory compounds in feedstock that have not been treated. These characteristics make this strain a potential microorganism for the production of lipids . There are several yeast species that can accumulate more than 20% of their dry weight in lipids.…”

Section: Introductionmentioning

confidence: 99%

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Challenges in lipid production from lignocellulosic biomass using Rhodosporidium sp.; A look at the role of lignocellulosic inhibitors

Osorio‐González

Hegde

Brar

et al. 2018

Biofuels Bioprod Bioref

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The use of lignocellulosic biomass biofuels is an attractive alternative because they do not put food safety at risk, they are a renewable source, and their use is limited. The use of microorganisms has now become more widespread to take advantage of the carbohydrates present in this raw material (cellulose and hemicellulose) and the products of its hydrolysis (glucose, xylose, arabinose, galactose, and mannose). The fatty acids obtained from oleaginous microorganisms are potential sources for the production of drop‐in biofuels. Rhodosporidium sp. is an oleaginous yeast that accumulates up to 70% of its biomass in the form of lipids and is highly adaptable to different types of substrates. This review discusses the different factors and challenges (genetic modification of strains, pretreatments, and inhibitor effects) in obtaining lipid from lignocellulosic biomass using Rhodosporidium sp. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

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Section: Feedstock Utilized By Rhodosporidium Sp To Produce Fatty Acmentioning

confidence: 99%

Section: Challenges In Lipid Production In Rhodosporidium Genrementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Challenges in lipid production from lignocellulosic biomass using Rhodosporidium sp.; A look at the role of lignocellulosic inhibitors

Osorio‐González

Hegde

Brar

et al. 2018

Biofuels Bioprod Bioref

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“…The oleaginous yeast can be readily cultivated in high-density cell cultures (1) and naturally accumulates large quantities of both carotenoids and lipids (2) which serve as precursors for many valuable compounds. Complementing this is the ability of R. toruloides to efficiently consume a wide variety of carbon sources, including those found in lignocellulose hydrolysates (3,4). Furthermore, R. toruloides can grow robustly under difficult environmental conditions, including high osmotic stress (5) and the presence of ionic liquids used in pretreatment processes (6)(7)(8).…”

mentioning

confidence: 99%

Multiplexed CRISPR-Cas9-Based Genome Editing of Rhodosporidium toruloides

Otoupal

Ito

Arkin

et al. 2019

mSphere

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Microbial production of biofuels and bioproducts offers a sustainable and economic alternative to petroleum-based fuels and chemicals. The basidiomycete yeast Rhodosporidium toruloides is a promising platform organism for generating bioproducts due to its ability to consume a broad spectrum of carbon sources (including those derived from lignocellulosic biomass) and to naturally accumulate high levels of lipids and carotenoids, two biosynthetic pathways that can be leveraged to produce a wide range of bioproducts. While R. toruloides has great potential, it has a more limited set of tools for genetic engineering relative to more advanced yeast platform organisms such as Yarrowia lipolytica and Saccharomyces cerevisiae. Significant advancements in the past few years have bolstered R. toruloides’ engineering capacity. Here we expand this capacity by demonstrating the first use of CRISPR-Cas9-based gene disruption in R. toruloides. Transforming a Cas9 expression cassette harboring nourseothricin resistance and selecting transformants on this antibiotic resulted in strains of R. toruloides exhibiting successful targeted disruption of the native URA3 gene. While editing efficiencies were initially low (0.002%), optimization of the cassette increased efficiencies 364-fold (to 0.6%). Applying these optimized design conditions enabled disruption of another native gene involved in carotenoid biosynthesis, CAR2, with much greater success; editing efficiencies of CAR2 deletion reached roughly 50%. Finally, we demonstrated efficient multiplexed genome editing by disrupting both CAR2 and URA3 in a single transformation. Together, our results provide a framework for applying CRISPR-Cas9 to R. toruloides that will facilitate rapid and high-throughput genome engineering in this industrially relevant organism. IMPORTANCE Microbial biofuel and bioproduct platforms provide access to clean and renewable carbon sources that are more sustainable and environmentally friendly than petroleum-based carbon sources. Furthermore, they can serve as useful conduits for the synthesis of advanced molecules that are difficult to produce through strictly chemical means. R. toruloides has emerged as a promising potential host for converting renewable lignocellulosic material into valuable fuels and chemicals. However, engineering efforts to improve the yeast’s production capabilities have been impeded by a lack of advanced tools for genome engineering. While this is rapidly changing, one key tool remains unexplored in R. toruloides: CRISPR-Cas9. The results outlined here demonstrate for the first time how effective multiplexed CRISPR-Cas9 gene disruption provides a framework for other researchers to utilize this revolutionary genome-editing tool effectively in R. toruloides.

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“…The oleaginous yeast can be readily cultivated in high-density cell cultures (1), and naturally accumulates large quantities of both carotenoids and lipids (2) which serve as precursors for many valuable compounds. Complementing this is the ability of R. toruloides to efficiently consume a wide variety of carbon sources, including those found in lignocellulose hydrolysates (3, 4). Furthermore, R. toruloides can grow robustly under difficult environmental conditions including high osmotic stress (5) and the presence of ionic liquids used in pretreatment processes (6–8).…”

mentioning

confidence: 99%