Exploration of A New Concept for Automated Dried Blood Spot Analysis Using Flow-Through Desorption and Online SPE–MS/MS

Ooms, Jan Albert; Knegt, Lena; Koster, Emile

doi:10.4155/bio.11.214

Cited by 45 publications

(36 citation statements)

References 18 publications

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“…However, this is equally true for direct elution, where online SPE, and 2D chromatography can also be utilised (109,103) . All these processes add considerable complexity, and the aim of this study was to investigate quick and simple methods of DBS analysis that are compatible with high throughput bioanalysis, which is often a critical factor in pharmaceutical discovery and development and other screening applications.…”

Section: Discussionmentioning

confidence: 99%

“…Heinig et al, and Ganz et al have both reported reliable automation performance and the ability to generate quantitative validation data within guideline acceptance criteria (101,102) . The DBS SPE-MS/MS from Spark Holland has been in development for a number of years and was first formally reported on in the literature in 2011 (103) . The concept is the same as that used in the SCAP system, the DBS sample is clamped from both sides and solvent is allowed to flow through to desorb the analyte.…”

Section: On-line Dbsmentioning

confidence: 99%

“…Subsequent research has further demonstrated the advantages on offer from DBS direct elution, and it's suitability to support regulated quantitative bioanalysis, and other applications (104,109,100,101,132,102,103) . Prototype semi-automated, and fully automated direct elution instruments have been used to demonstrate that the technique offers comparable reliability to conventional manual extraction (75) .…”

Section: Introductionmentioning

confidence: 99%

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Direct Analysis of Dried Blood Spot Samples

Abu-Rabie¹

2014

Dried Blood Spots

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Section: Discussionmentioning

confidence: 99%

Section: On-line Dbsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Direct Analysis of Dried Blood Spot Samples

Abu-Rabie¹

2014

Dried Blood Spots

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“…The reduced risk of infection, due the fact that pathogens are deactivated upon drying [28], further contributes to the convenient and cost-effective transport and storage. From an analytical point of view, compounds can often be extracted from DBS cards using straightforward procedures, making this technique particularly suitable for automated processing [29][30][31].…”

Section: Dried Blood Spotsmentioning

confidence: 99%

Alternative Sampling Strategies for Cytochrome P450 Phenotyping

2015

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Interindividual variability in the expression and function of drug metabolizing cytochrome P (CYP) 450 enzymes, determined by a combination of genetic, non-genetic and environmental parameters, is a major source of variable drug response. Phenotyping by administration of a selective enzyme substrate, followed by the determination of a specific phenotyping metric, is an appropriate approach to assess the in vivo activity of CYP450 enzymes, as it takes into account all influencing factors. A phenotyping protocol should be as simple and convenient as possible. Typically, phenotyping metrics are determined in traditional matrices, such as blood, plasma or urine. Several sampling strategies have been proposed as an alternative for these traditional sampling techniques.In this review, we provide a comprehensive overview of available methods using dried blood spots, hair, oral fluid, exhaled breath and sweat for in vivo CYP450 phenotyping. We discuss the relation between phenotyping metrics measured in these samples and those in conventional matrices, along with the advantages and limitations of the alternative sampling techniques. Reliable phenotyping procedures for several clinically relevant CYP450 enzymes, including CYP1A2, CYP2C19 and CYP2D6, are currently available for oral fluid, breath or dried blood spots, while additional studies are needed for other CYP450 isoforms, such as CYP3A4. The role of hair analysis for this purpose remains to be established. Being non-or minimally invasive, these sampling strategies provide convenient and patient-friendly alternatives for classical phenotyping procedures, which may contribute to the implementation of CYP450 phenotyping in clinical practice. 4 Key Points Phenotyping by administration of a selective probe drug, followed by determination of a specific phenotyping metric, allows to assess the in vivo enzyme activity of CYP450 enzymes, taking into account genetic, non-genetic and environmental influencing factors. In addition to classical sampling techniques, such as blood or urine collection, reliable phenotyping procedures for several clinically relevant CYP450 enzymes are currently available for oral fluid, breath and dried blood spots.

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“…It was demonstrated that a significant improvement of drug recoveries could be obtained by application of heated flow-through desorption. Elevated recovery rates were observed even when a hematocrit range between 0.25 and 0.65 L/L were used [10,11].…”

mentioning

confidence: 95%