Exploration of bacteria associated with Nudibranchs to control Vibrio spp.

Sarjito, Sarjito; Prayitno, Slamet Budi; Farisa, M Y; Nast, R. T. C.; Kristiana, Rhesi; Sabdaningsih, Aninditia; Sabdono, Agus

doi:10.1088/1755-1315/530/1/012031

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…Another study from Indonesia described the anti-MRSA, cytotoxicity, and anti-HSV-1 activity of bacterial symbionts from the viscera of nudibranchs . The presence of bacterial symbionts in a nudibranch to inhibit the growth of pathogenic Vibrio species (Prayitno et al, 2020) was also revealed. In addition, the nudibranch Rostanga alisae harbors a high diversity of specific endo-and extracellular bacteria, and some of them are previously unknown as symbionts of marine invertebrates (Zhukova et al, 2022).…”

Section: Natural Products Of Mollusks' Bacterial Symbiontsmentioning

confidence: 97%

Marine mollusks are a good source of natural products

Mahmoud,

Abdelrahman,

Hanora

et al. 2023

Records of Pharmaceutical and Biomedical Sciences

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Almost all marine ecosystems have Mollusca members for their balanced continued functioning. While some may seem harmless or even gentle, others are equipped with powerful weapons like venomous tentacles to fend off predators. Mollusks are considered an important subject of research in various fields due to their unique biology and ecological significance. Their research has many implications in areas such as medicine and biotechnology. Numerous species have recently been the subject of research, with numerous secondary metabolites being isolated and their chemical structures being clarified. Natural substances that were poisonous to tumor cells, harmful bacteria, viruses, and others have been discovered. Some isolated compounds constituted the active ingredients in medicinal preparations used in clinical practice, while others are still in various stages of preclinical research. These substances often lacked equivalents in other naturally occurring substances with terrestrial origin. Metagenomics tools has simplified the discoveries in this field and with the help of metagenomics, we could identify new genes and proteins to be used in biotechnology applications, such as the development of new drugs.

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Section: Natural Products Of Mollusks' Bacterial Symbiontsmentioning

confidence: 97%

Marine mollusks are a good source of natural products

Mahmoud,

Abdelrahman,

Hanora

et al. 2023

Records of Pharmaceutical and Biomedical Sciences

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“…Dari isolat tersebut hanya satu isolat (SM-N-3(2)/7) yang teridentifikasi memiliki kemampuan melawan V.harveyi dan V. parahaemolyticus. Isolatnya adalah bakteri batang gram negatif, berdasarkan identifikasi molekuler menggunakan 16S rRNA itu terkait erat dengan Pseudoalteromonas piscicida strain NBRC (99%) (Sarjito, et al, 2020). Penelitian lain menunjukkan bahwa ekstrak kasar dari supernatan kultur Pseudoalteromonas rubra dan V. salarius, simbion dari nudibranch, memiliki aktivitas anti-MRSA, yang pasti disebabkan oleh senyawa yang diproduksi dan disekresikan oleh bakteri.…”

Section: Hasil Dan Pembahasanunclassified

Keanekaragaman dan Kelimpahan Nudibranch di Pantai Penimbangan Buleleng Bali

2022

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Pantai Penimbangan terletak Desa Baktiseraga, Kecamatan Buleleng merupakan kawasan pusat kota Kabupaten Buleleng, Bali yang memiliki hamparan pasir hitam. Kegiatan konservasi penyu di kawasan ini meningkatkan gairah masyarakat dalam menjaga kelestarian ekosistem terumbu karang. Salah satu biota yang beriteraksi di ekosistem terumbu karang adalah nudibranch. Keberadaan nudibranch di Pantai Penimbangan diharapkan mampu menjadi alternatif potensi wisata yang bisa dikembangkan, sehingga antusiasisme masyarakat untuk menjaga kelestarian ekosistem pesisir akan terus terjaga. Penelitian bertujuan untuk memperkuat data dasar ekologi tentang keanekaragaman dan kelimpahan nudibranch. Penelitian populasi nudibranch dinilai dengan sensus visual mengikuti line intercept transect. Penelitian nudibranch di Pantai Penimbangan menunjukkan bahwa terdapat 12 famili yaitu Chromodorididae, Phyllidiidae, Discodorididae, Aeolidiidae, Elysiidae, Aglajidae, Bornellidae, Facelinidae, Flabellinidae, Hexabranchidae, Limapontiidae, dan Pleurobranchidae, dengan 42 jenis. Kelompok Chromodorididae dan Phyllidiidae adalah kelompok yang umum dijumpai di Perairan Pantai Penimbangan. Kedua kelompok ini memanfaatkan patahan karang yang menjadi subsrat perairan sebagai tempat berlindung. Komposisi substrat sangat menentukan kekayaan nudibranch, kategori substrat yang menentukan keberadaan nudibranch adalah dead coal with alga, sponge, dan hard coral. Tingginya persentase dead coral with alga, dan sponge berbanding lurus dengan keanekaragaman nudibranch, dan hard coral berbanding terbalik dengan keanekaragaman nudibranch. Kriteria indeks keanekaragaman tinggi dengan nilai 2,6199, Indeks keseragaman 0,7055 dan nilai dominansi 0,0342. Tingkat kelimpahan tertinggi pada jenis Hypselodoris tryoni. Penimbangan Beach is located in Baktiseraga Village, Buleleng District, which is the downtown area of Buleleng Regency, Bali which has a stretch of black sand. Turtle conservation activities in this area increase the enthusiasm of the community in preserving the coral reef ecosystem. One of the biota that interacts in the coral reef ecosystem is the nudibranch. The existence of nudibranchs at Penimbangan Beach is expected to be an alternative tourism potential that can be developed, so that public enthusiasm for preserving coastal ecosystems will continue to be maintained. This study aims to strengthen the basic ecological data on the diversity and abundance of nudibranchs. The study of the nudibranch population was assessed by visual census following the line intercept transect. Research on nudibranchs at Penimbangan Beach showed that there were 12 families namely Chromodorididae, Phyllidiidae, Discodorididae, Aeolidiidae, Elysiidae, Aglajidae, Bornellidae, Facelinidae, Flabellinidae, Hexabranchidae, Limapontiidae, and Pleurobranchidae, with 42 species. Chromodorididae and Phyllidiidae groups are common groups found in Penimbangan Beach. Both of these groups take advantage of coral fractures which become the substrate of the waters as a shelter. Substrate composition greatly determines the richness of nudibranchs, the substrate categories that determine the presence of nudibranchs are dead coral with algae, sponges, and hard corals. The high percentage of dead coal with algae, and sponges is directly proportional to nudibranch diversity, and hard coral is inversely proportional to nudibranch diversity. The criteria for the high diversity index are 2.6199, the uniformity index is 0.7055 and the dominance value is 0.0342. The highest abundance level was in Hypselodoris tryoni.

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“…We previously investigated the potential of nudibranch-associated bacteria against Vibrio harveyi and V. parahaemolyticus. Pseudoalteromonas piscicida isolated from nudibranchs had inhibitory activity of 19.4 and 10.63 mm, respectively (Sarjito et al 2020). In the marine nutrient cycle, a sponge, which lives as a filter feeder, plays a zoobenthic roles, allowing marine microbes to easily infiltrate and reside in the sponge body.…”

Section: Introductionmentioning

confidence: 99%

Screening of sponge-associated bacteria to control vibriosis in vannamei shrimp (Litopenaeus vannamei)

2022

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Abstract. Sarjito S, Amalia R, Sabdaningsih A. 2022. Screening of sponge-associated bacteria to control vibriosis in vannamei shrimp (Litopenaeus vannamei). Biodiversitas 23: 5333-5341. An intensive shrimp culture with a high stocking density causes a higher chance of vannamei shrimp being infected with diseases. The most frequent disease is caused by Vibrio sp. Vibrio might cause a severe production loss in the shrimp culture, leading to larvae and mature stadium mortality at 50%. Therefore, a study on the alternative management of vibriosis disease using biocontrol agents is urgently needed. The sponge is a marine invertebrate that provides as a shelter for microbes to live and symbiosis with the animal. An evident ability of sponge-associated bacteria to produce antimicrobial compounds can be exploited as a biodegradable bio-agent, potentially as a solution to control vibriosis disease in a shrimp. This research aimed to isolate sponge-associated bacteria with vibriosis antibacterial potential. Sampling was done in Tulamben, Bali and Panjang island, Jepara, Indonesia with six sampling sites consisting of 15 sampling points. Moreover, vibriosis bacteria were provided by the laboratory of fish disease at Balai Besar Perikanan Budidaya Air Payau (BBPBAP) Jepara. Totally, 24 and 47 pure cultures were successfully isolated using Zobell 2216E agar medium from Tulamben, Bali and Panjang Island, Jepara respectively. The isolates were tested for antibacterial assay using plug agar diffusion methods. The top eight potential isolates were found in isolate B6.3C, B6.3D, B6.4E, B9.3C, PA.1, PA.4, PC.3, and PH.1 against Vibrio vulnificus, V. anguillarum, V. alginolyticus, V. parahaemolyticus, however there was no activity in V. harveyi. The molecular method was carried out to identify those potential isolates using universal primer 16S rRNA. The BLAST homology showed that all potential antibacterial isolates belong to the family Bacilllaceae, such as Bacillus cereus, Virgibacillus salarius, B. aerius, B. paramycoides, B. thuringiensis, and B. altitudinis. Further research will be carried out on microencapsulation and in vivo tests.

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Exploration of bacteria associated with Nudibranchs to control Vibrio spp.

Cited by 3 publications

References 18 publications

Marine mollusks are a good source of natural products

Marine mollusks are a good source of natural products

Keanekaragaman dan Kelimpahan Nudibranch di Pantai Penimbangan Buleleng Bali

Screening of sponge-associated bacteria to control vibriosis in vannamei shrimp (Litopenaeus vannamei)

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